Transfering vial rubber piece , vacuum question

Discussion in 'Steroid Homebrew' started by Kinezz, Nov 16, 2018.

  1. Kinezz

    Kinezz Junior Member

    Was wondering after I use the vacuum can I just open the rubber off the vial instead of pinning it with a bigger draw and smaller syringe or will it get contaminated I've had a few issues with the rubber piece falling out in to the vial so yeh
     
  2. master.on

    master.on Member

    More chance for contamination
    OTOH amps are meant to be opened and there are no horror stories.

    But amps are single use. Are you going to inject everything in the vial at once?

    For ultimate sterility, you can open the vial and work next to a bunsen burner, or maybe a propane torch on the cheap.

    After the disinfectant has dried completely, use an igniter to light the Bunsen burner. Adjust the flame so that a blue cone can be seen in the middle of the flame. The flame is now producing an updraft, or air convection currents in which warm air rises up and away from the flame (Figure 1). As heat rises, microorganisms and dust particles are forced upward and away from the immediate work area. Work slowly, carefully, and deliberately at all times within this area created by the Bunsen burner, referred to as a sterile field. Keep the Bunsen burner on during the entire procedure.
    [​IMG]
    Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941987/bin/jove-63-2754.mp4
     
    MisterSuperGod likes this.
  3. master.on

    master.on Member

    On the cheap a propane torch works well
    but the flame sits higher.

    [​IMG]

    Please be careful so that it doesn't tip over
    or buy a stand for it

    [​IMG]

    [​IMG]
     
  4. Kinezz

    Kinezz Junior Member

    So no way I'd be able to take it out I guess without this methods
     
  5. Holidaypay

    Holidaypay Member

    You say some weird shit man but I think I'm starting to like you that's interesting I never thought about it
     
    master.on likes this.