Reconstituting and Storing HGH/Reta

[seelos]

Sorry, I'm sure this is discussed a lot but there's so much conflicting opinions online I wasn't sure what to do.

I have 310iu HGH, 5 10mg vials of reta, and 20ml bac water.
I was planning on storing the powder form HGH in a dark cold drawer in an unused room, and keeping the powder reta/bac in the fridge. and then of course the reconstituted hgh/reta in the fridge.
For reconstitution I was thinking 0.6ml bac for 31iu (assuming 30iu) for a simple 50iu/ml
and then the reta 0.4ml bac 10mg vials for 25mg/ml

and i was gonna start off at 2iu hgh daily 2mg reta weekly just to monitor reaction and sides, gradually go up.
sorry, this is just my first time and im scared to mess things up

 

[Stubb]

I would add more bac into both of those.

3.1ml into the 31iu hgh for 10iu/ml. Giving you 0.2ml per dose - a lot easier to dose than the concentration you've gone for.

1-2ml for the reta. You do get some wastage in the syringe after injection and a higher concentration would reduce that.

Also the 31iu vial, at the dosage you'll be starting with anyway will last you just over two weeks, which is kinda on the long side for reconstituted gh - but still OK.

Apart from those suggestions everything else is looking good.

 

[Photon]

Sorry, I'm sure this is discussed a lot but there's so much conflicting opinions online I wasn't sure what to do.

I have 310iu HGH, 5 10mg vials of reta, and 20ml bac water.
I was planning on storing the powder form HGH in a dark cold drawer in an unused room, and keeping the powder reta/bac in the fridge. and then of course the reconstituted hgh/reta in the fridge.
For reconstitution I was thinking 0.6ml bac for 31iu (assuming 30iu) for a simple 50iu/ml
and then the reta 0.4ml bac 10mg vials for 25mg/ml

and i was gonna start off at 2iu hgh daily 2mg reta weekly just to monitor reaction and sides, gradually go up.
sorry, this is just my first time and im scared to mess things up

I assume its
31IU x 10 vials of HGH?

It almost sounds like you bought 310IU of pure powder when you mention 50iu/ml. Most people will produce more than 2 IU of HGH daily, so taking it would be a waste since adding it will just suppress natural production.

 

[Usman457]

Yeah put 1ml of bac into the Reta, makes life easier, so 0.1 is 1mg ect, store that in the fridge

 

[Ghoul]

Store it all in the fridge. Colder temps slow chemical degradation. It's only upside.

Look to pharma practices for guidance. Surely with billions spent on development and thousands of scientists working on this stuff, there's a chance they might know what they're doing.

Excess concentration causes multiple problems. One is faster degradation. The other is poor pharmacokinetics. The side effects most commonly associated with rHGH are linked to sharp GH spikes after administration. Higher concentrations are absorbed more quickly, leading to a sharper spike. Lower concentrations have a smaller spike and more gradual curve, with fewer sides when it's absorbed more gradually.



No commercial rHGH comes in a higher concentration than 36iu/ml, and that's with a package of stabilizers to protect from aggregation yours doesn't have.

18-20iu/ml is much more common for rHGH.

Eli Lily's highest Reta concentration is than 12mg per .5ml, so your 25mg/ml is close to what they're using.

In general, greater dilution of proteins, as long as the volume is reasonable for each injection, isn't going to hurt anything, and slows degradation.

 

[Stealth500]

I would add more bac into both of those.

3.1ml into the 31iu hgh for 10iu/ml. Giving you 0.2ml per dose - a lot easier to dose than the concentration you've gone for.

1-2ml for the reta. You do get some wastage in the syringe after injection and a higher concentration would reduce that.

Also the 31iu vial, at the dosage you'll be starting with anyway will last you just over two weeks, which is kinda on the long side for reconstituted gh - but still OK.

Apart from those suggestions everything else is looking good.

you can eliminate further wastage left in the syringe by diluting the dose immediately before injection with more bac water after drawing the required dosage.

 

[seelos]

Store it all in the fridge. Colder temps slow chemical degradation. It's only upside.

Look to pharma practices for guidance. Surely with billions spent on development and thousands of scientists working on this stuff, there's a chance they might know what they're doing.

Excess concentration causes multiple problems. One is faster degradation. The other is poor pharmacokinetics. The side effects most commonly associated with rHGH are linked to sharp GH spikes after administration. Higher concentrations are absorbed more quickly, leading to a sharper spike. Lower concentrations have a smaller spike and more gradual curve, with fewer sides when it's absorbed more gradually.

View attachment 333139

No commercial rHGH comes in a higher concentration than 36iu/ml, and that's with a package of stabilizers to protect from aggregation yours doesn't have.

18-20iu/ml is much more common for rHGH.

Eli Lily's highest Reta concentration is than 12mg per .5ml, so your 25mg/ml is close to what they're using.

In general, greater dilution of proteins, as long as the volume is reasonable for each injection, isn't going to hurt anything, and slows degradation.

Very informative reply, thank you. So updated routine of 1.5ml bac per each 30iu bottle (31, idk if it better to round down or up if it’s listed as 31 iu) for 20iu/ml, 10 units for my 2iu dose.
and the. just 0.5ml/vial for reta, 20mg/ml also 10 units for my 2mg dose. below eli lily max, and simple math.

with all that covered, do you know if the 2iu daily / 2mg weekly is a fine starting dose? the other person mentioned that 2iu could lower my levels

 

[Ghoul]

Very informative reply, thank you. So updated routine of 1.5ml bac per each 30iu bottle (31, idk if it better to round down or up if it’s listed as 31 iu) for 20iu/ml, 10 units for my 2iu dose.
and the. just 0.5ml/vial for reta, 20mg/ml also 10 units for my 2mg dose. below eli lily max, and simple math.

with all that covered, do you know if the 2iu daily / 2mg weekly is a fine starting dose? the other person mentioned that 2iu could lower my levels

Exogenous GH will suppress natural production, and too little will leave you worse off. 2iu is the top of the range for adult gh deficiency treatment and around 1.5 is the max amount a healthy male would produce daily, So less than that could leave you worse off.

There's also the fact 2iu may not be a full 2iu of activity when it comes to UGL. I always assume it's less reliable than pharma.

So all of that said, to make sure you're actually increasing from current levels, if you want a conservative start, imo go with 3iu. Thats still very low and safe, but enough to compensate for any weakness in the UGL rHGH potency and still be fairly certain that it's enough to *do* something without going too far into the supra-physiologic range where risks and complexity of managing it increase.

I see you're 20. rHGH benefits tend to slowly accumulate over time, not bursts of dramatic improvements like AAS, In your case you're at your lifetime peak of natural GH so benefits will be even more subtle vs. a 35-40yo with far lower levels., You're already living the benefits of high GH levels. Just something to consider.

 

[seelos]

Exogenous GH will suppress natural production, and too little will leave you worse off. 2iu is the top of the range for adult gh deficiency treatment and around 1.5 is the max amount a healthy male would produce daily, So less than that could leave you worse off.

There's also the fact 2iu may not be a full 2iu of activity when it comes to UGL. I always assume it's less reliable than pharma.

So all of that said, to make sure you're actually increasing from current levels, if you want a conservative start, imo go with 3iu. Thats still very low and safe, but enough to compensate for any weakness in the UGL rHGH potency and still be fairly certain that it's enough to *do* something without going too far into the supra-physiologic range where risks and complexity of managing it increase.

I see you're 20. rHGH benefits tend to slowly accumulate over time, not bursts of dramatic improvements like AAS, In your case you're at your lifetime peak of natural GH so benefits will be even more subtle vs. a 35-40yo with far lower levels., You're already living the benefits of high GH levels. Just something to consider.

Thank you, i’ll start with 3iu and monitor, good to note that my gh could already be high

 

[Ghoul]

Thank you, i’ll start with 3iu and monitor, good to note that my gh could already be high

Your best bet would to get an inexpensive IGF blood test so you have a baseline before starting.

 

[seelos]

Your best bet would to get an inexpensive IGF blood test so you have a baseline before starting.

I got all my bloods done around a week ago I’ll likely get them again in two months.
Sorry but last question, what’s considered cloudy hgh? I put in the 1.5ml bac around an hour ago i’ve been letting it sit in a cool dark place, and it doesn’t look as clear as my retatrutide. It’s from lobster. definitely not bad by any means but comparing side by side with the reta there is a difference - should i just let it sit out and dissolve longer?

 

[Ghoul]

I got all my bloods done around a week ago I’ll likely get them again in two months.
Sorry but last question, what’s considered cloudy hgh? I put in the 1.5ml bac around an hour ago i’ve been letting it sit in a cool dark place, and it doesn’t look as clear as my retatrutide. It’s from lobster. definitely not bad by any means but comparing side by side with the reta there is a difference - should i just let it sit out and dissolve longer?

It should be crystal clear upon reconstitution. (though that's not really a guarantee of anything).

"Cloudiness" is aggregation of rHGH monomers reaching the point where they become visible (many millions of rHGH monomer molecules stuck to each other to become a single visible speck).

There could easily be unseen "cloudiness" formed by aggregates below the level visible by the naked eye.

What's the size of those vials in iu?

 

[seelos]

It should be crystal clear upon reconstitution. (though that's not really a guarantee of anything).

"Cloudiness" is aggregation of rHGH monomers reaching the point where they become visibile (many millions of rHGH monomer molecules to reach reach the point of becoming a single visible speck).

There could easily be "cloudiness" formed by aggregates below the level visible by the naked eye.

What's the size of those vials in iu?

it’s the lobster 31iu vials, the lab tests on his site show 31.8iu specifically. i’ve been very gentle with the vials just lightly rolling between fingers after putting the full 1.5ml in, that wouldn’t have caused anything right?

 

[Ghoul]

it’s the lobster 31iu vials, the lab tests on his site show 31.8iu specifically. i’ve been very gentle with the vials just lightly rolling between fingers after putting the full 1.5ml in, that wouldn’t have caused anything right?

I recommend reconstituting with 3ml.

Most formulations of rHGH don't exceed 12iu per 1ml, in part to prevent aggregation. Even if they've aggregated at the higher concentration you have it at now, that's often reversible when dilution is increased.

And clear or not, I always recommend filtration to minimize aggregates (and other sub-visible crap) from being injected. No need for that stuff to accumulate in your body on a daily basis. Only takes a minute once you have the process down.

 

[seelos]

I recommend reconstituting with 3ml.

Most formulations of rHGH don't exceed 12iu per 1ml, in part to prevent aggregation. Even if they've aggregated at the higher concentration you have it at now, that's often reversible when dilution is increased.

And clear or not, I always recommend filtration to minimize aggregates (and other sub-visible crap) from being injected. No need for that stuff to accumulate in your body on a daily basis. Only takes a minute once you have the process down.

added another 1.5ml to get that to 3, it's looking pretty good now

 

[Ghoul]

added another 1.5ml to get that to 3, it's looking pretty good now


View attachment 333196

Yeah, you probobly recovered most of what had aggregated. Make sure to dilute the rest of the vials the same way, because the longer soluble aggregates go on in that form, the more likely the weak bonds can form permanent ones.

And consider ordering filters for future use

25mm .22um (not the .65 prefilter version.).

Sterile 0.2 μm PES Syringe Filters

Hydrophilic PES syringe filters with single or double-layer membrane. Pore sizes of 0.2μm. Diameter of 13mm/25mm/33mm with Luer lock. Sterile individually packed.

shop.cobetter.com

 

[Ateam2023]

I got all my bloods done around a week ago I’ll likely get them again in two months.
Sorry but last question, what’s considered cloudy hgh? I put in the 1.5ml bac around an hour ago i’ve been letting it sit in a cool dark place, and it doesn’t look as clear as my retatrutide. It’s from lobster. definitely not bad by any means but comparing side by side with the reta there is a difference - should i just let it sit out and dissolve longer?

what was your "baseline igf1"? Or are you waiting on the results? Don't know if i missed something,,

 

[Photon]

Thank you, i’ll start with 3iu and monitor, good to note that my gh could already be high

Not sure if i would recommend using hgh to anyone at 20yo but I'm sure you've done more research than me since it's your body. Good luck!

 

[Ghoul]

Not sure if i would recommend using hgh to anyone at 20yo but I'm sure you've done more research than me since it's your body. Good luck!

For the record I agree, he's got plenty of natural gh to work with, but we all know nobody's going to "tell us what to do" once we've made up our mind, so minimizing harm for the inevitable is the next best thing imo.

If weight loss and recomp is the goal here, GLP is plenty of an assist, and diet and exersize will get him the rest of the way to where he wants to be fast.

 

[seelos]

For the record I agree, he's got plenty of natural gh to work with, but we all know nobody's going to "tell us what to do" once we've made up our mind, so minimizing harm for the inevitable is the next best thing imo.

If weight loss and recomp is the goal here, GLP is plenty of an assist, and diet and exersize will get him the rest of the way to where he wants to be fast.

You're probably right, but I appreciate the sentiment of trying to help me reduce risk rather than just tell me to not do it.

I just went to do my first injection, and it's coming out like this into the needle. I tried drawing twice, both times had this white milky substance. It's not a bubble, I injected it into my sink and at the end it was very clearly white and thicker coming out. I'm assuming this isn't normal? Didn't inject it into me of course, but any idea what it is/how it happened?