Search results for query: Circular dichroism

...if you go this route. All you will get is the data and Jano won't interpret. Very tedious analysis work. I was headed down the circular dichroism path which requires a pharma standard to compare UG sample against. Interpretation much simpler...

...Various additional testing has been kicked around in the context of pharma vs UG GH... 1. NMR / Circular dichroism (HGH structure) 2. Nb2 cell line assay or reporter gene assay (biological activity of GH in IU/mg) 3. Host cell protein assays

Wouldn't secondary structure be useless without tertiary structure since that's what matters?

readalots back,,,

...did the leg work with Jano. He doesn't have access to Nb2 cell line proliferation assay but does have a lab that will do the circular dichroism comparison between pharma standard and UG GH sample to compare secondary structure. Better bang for buck than NMR and Native PAGE unless you have...

At $25-30 a test it would be nice to see vendors offer double or triple credit for serum GH testing (for time and inconvenience). I think this was fairly common before Jano testing became the norm. Obviously it's not an infallible test, mostly because of the human factor, but it really gets...

Whatever works. You can see in the FDA rHGH approval docs every pharma company seems to submit a set of tests done using different techniques, so there's more than one way to skin a cat. Too bad about bioactivity though. If you could only perform one test, THAT would be the one that gives you...

Welcome back to the MESO community (I assume you took a little break as I hadn't seen you post for a while). I did my best to get answers to your questions so I'm glad you were able to find that reply. I'm also happy to see you found these GH analysis results. Your inquires and our discussions...

Thanks for sharing these results. Is your host E Coli or Mammalian cell based? Any other analytical testing performed on your GH? NMR, circular dichroism? What internal testing is performed for QC/QA? Any assays to screen for host cell proteins in the final product?

I learned about this in basic high school science class. I wrote a quick summary of what I know about it. No biggie Circular Dichroism (CD) and Nuclear Magnetic Resonance (NMR) are both useful for studying peptides, but they serve different purposes: - CD is great for quickly checking the...

I love the internet

If you want me to actually understand that, I'll need it explained like its from woody from toy story explaining it to buzz

Thanks. My read was we can get some 3D info out of CD using near UV vs far UV. Of course not as detailed as NMR. But all this dependent on what CD methods the lab offers and the CD testing has a time horizon (whereas the NMR testing does not)...

Curious if @janoshik even offers a way to check this for us Chatgpt:

Does that mean you buy the GH raws from China and then lyophilize it in your own facilities in Turkey? Not having a vaccum in the vials could in principle lead to faster degradation through oxidation (unless the vial contains an inert gas like nitrogen). See below. So this should be of concern...

...conditions of knowing that. You can identify stuff by many other - some better suited - tests. Retention time being the most common with chromatography, fragmentation patterns, circular dichroism etc... However these all have a condition that AAA does not have - you need to have a standard.

...an artifact in there, but all the other methods I have researched. I don't know much about this except it's similar to RP. CD (circular dichroism) is good tool, but very impractical. IEF provides very little useful information. If I am understanding correctly, the SEC and RP-HPLC is used...

...is usually analyzed by optical spectroscopy, such as UV spectroscopy, fluorescence spectroscopy, optical rotary dispersion, or circular dichroism. These methods are generally not sensitive enough to detect the subtle conformational changes caused by small alterations in the protein structure...