Filtering Multiple Times

[RoidedUpEbi]

I keep hearing from people that this lab or that lab triple filters their oils. Does filtering oils multiple times actually make any difference? I'd assume filtering through 0.22um filters multiple times wouldn't make a difference.

Also, is there any benefit to filtering multiple times with different types of filters with the same size? I.e Nylon 0.22um then 0.22um PTFE?

 

[Ghoul]

I keep hearing from people that this lab or that lab triple filters their oils. Does filtering oils multiple times actually make any difference? I'd assume filtering through 0.22um filters multiple times wouldn't make a difference.

Also, is there any benefit to filtering multiple times with different types of filters with the same size? I.e Nylon 0.22um then 0.22um PTFE?

.22um filters prevent bacteria from contaminating your gear, making it sterile, except for all the species of bacteria smaller than .22um.

Broad diversity of viable bacteria in 'sterile' (0.2 microm) filtered water - PubMed

Filtration of liquids through 0.2-microm filters is a common and often-used method for the removal of microorganisms from heat-sensitive solutions. Such 0.2 microm filtrations are frequently referred to as 'sterile filtration', reflecting the general belief that all living organisms are excluded...

pubmed.ncbi.nlm.nih.gov

These are the shards from typical cheap borosilicate glass vial delamination, caught in a filter and viewed under a microscope. So maybe the best plan would be to filter your brew once, then use a syringe filter to draw into your syringe immediately before pinning. Or not. I'm sure this glass is broken down and digested once it's injected, somehow...or maybe it stabs the bacteria that get through the .22 filter to death so the risk evens out in the end.

 

[TRT]

.22um filters prevent bacteria from contaminating your gear, making it sterile, except for all the species of bacteria smaller than .22um.

Broad diversity of viable bacteria in 'sterile' (0.2 microm) filtered water - PubMed

Filtration of liquids through 0.2-microm filters is a common and often-used method for the removal of microorganisms from heat-sensitive solutions. Such 0.2 microm filtrations are frequently referred to as 'sterile filtration', reflecting the general belief that all living organisms are excluded...

pubmed.ncbi.nlm.nih.gov

These are the shards from typical cheap borosilicate glass vial delamination, caught in a filter and viewed under a microscope. So maybe the best plan would be to filter your brew once, then use a syringe filter to draw into your syringe immediately before pinning. Or not. I'm sure this glass is broken down and digested once it's injected, somehow...or maybe it stabs the bacteria that get through the .22 filter to death so the risk evens out in the end.

View attachment 296173

Lmao! “Stabs it to death.”

 

[Ghoul]

Lmao! “Stabs it to death.”

Yeah I just checked the contents of my syringe and it's a legit way to sterilize. After shaking it, looks like those sub .22um bugs won't be infecting anything anymore.

 

[TA13579]

This is terrifying. So I've been injecting glass shards this whole time?

This homebrew section of the forum is very educational, but also nightmare fuel.