Slammich
New Member
Some use alcohol before the oven, other use distilled water before the oven after alcohol. Pro advice, anyone?
MESO-Rx
Anabolic Steroids
Final rinse for glassware?
- Thread starter Slammich
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Either one should work tbh. I've used distilled water and iso alcohol 71% and have had the same reault each time
Slammich
New Member
Nooe. Both are clean as a whistle
Distillation is vapor so nothing else is with the water vapor, just gross tasteless water. Iso alcohol is what it is.
Distillation is vapor so nothing else is with the water vapor, just gross tasteless water. Iso alcohol is what it is.
Slammich
New Member
I think I will go with alcohol then pre oven. Fuck I'm excited. Thanks for the pro tip.
Good luck. Don't be afraid to say fuck it and start over. I've recleaned so many little vials out of fear. Worth it if you have any doubt about how sterile it is.
Slammich
New Member
Will do. Not trying to get the funk off my own brew that's for sure.
LordSamuilo
Member
@Slammich This right here is great advice . I've had a few incidents were I rinsed with iso alcohol and baked the hell outta my vials . I knew 99.9% for sure that they were sterililized , but when i pulled them out there were a few sketchy looking vials so i just started from scratch ... Better to be a little anal retentive with something your going to inject into your body , then end up getting a chunk of your ass cut out .
master.on
New Member
Define glassware
if it's beakers, graduated cylinders or anything used before filtering
then washing with soap (some prefer to rinse in alcohol to dissolve oil), rinse with tap water, then rinse with distilled water to avoid water marks. Allow to dry upside down so excess water can pour out, then briefly dry top up to allow water vapor to escape.
Now if by glassware you mean anything after filtering
like vials, media bottles, pipettes (for transferring from media bottle to vials)
first see if you can buy pre-sterile.
If not
1 boil in hydrogen peroxide solution
2 some rinse in distilled water, others don't
3 rinse with alcohol
4 allow excess alcohol to drip down
5 Dry. Some like to allow alcohol to dry by itself, others (like me) like to lit alcohol on fire with a flame, for further sterilization (make sure alcohol ain't dripping anymore so glass only has the little alcohol that adheres to it. This scarce alcohol makes the flame so short lasting, it won't break glass.
Hope this helps.
Dirtyaxiom
New Member
i just say screw it and toss out the media bottles when im done with them and get new ones.
Slammich
New Member
I got it done, and am comfortable with the method I used. I appreciate all the input. A few more runs and I should have an easy system in place.
master.on
New Member
Just an important comment
vials/bottles should be WRAPPED when placed in the oven, otherwise bacteria will get back on them, especially after taking them out of the oven.
Aluminum foil usually works fine.
It avoids airborne bacteria, while allowing heat to pass thru.
Of course wrapping is counterproductive in an actual autoclave as it prevents vater vapor from getting it.
Dry heat
See also: Dry heat sterilization
Dry heat sterilizer
Dry heat was the first method of sterilization and is a longer process than moist heat sterilization. The destruction of microorganisms through the use of dry heat is a gradual phenomenon. With longer exposure to lethal temperatures, the number of killed microorganisms increases. Forced ventilation of hot air can be used to increase the rate at which heat is transferred to an organism and reduce the temperature and amount of time needed to achieve sterility. At higher temperatures, shorter exposure times are required to kill organisms. This can reduce heat-induced damage to food products.[14]
The standard setting for a hot air oven is at least two hours at 160 °C. A rapid method heats air to 190 °C for 6 minutes for unwrapped objects and 12 minutes for wrapped objects.[15][16] Dry heat has the advantage that it can be used on powders and other heat-stable items that are adversely affected by steam (e.g. it does not cause rusting of steel objects).
Sterilization (microbiology) - Wikipedia
Hot air oven - Wikipedia
vials/bottles should be WRAPPED when placed in the oven, otherwise bacteria will get back on them, especially after taking them out of the oven.
Aluminum foil usually works fine.
It avoids airborne bacteria, while allowing heat to pass thru.
Of course wrapping is counterproductive in an actual autoclave as it prevents vater vapor from getting it.
Dry heat
See also: Dry heat sterilization
Dry heat sterilizer
Dry heat was the first method of sterilization and is a longer process than moist heat sterilization. The destruction of microorganisms through the use of dry heat is a gradual phenomenon. With longer exposure to lethal temperatures, the number of killed microorganisms increases. Forced ventilation of hot air can be used to increase the rate at which heat is transferred to an organism and reduce the temperature and amount of time needed to achieve sterility. At higher temperatures, shorter exposure times are required to kill organisms. This can reduce heat-induced damage to food products.[14]
The standard setting for a hot air oven is at least two hours at 160 °C. A rapid method heats air to 190 °C for 6 minutes for unwrapped objects and 12 minutes for wrapped objects.[15][16] Dry heat has the advantage that it can be used on powders and other heat-stable items that are adversely affected by steam (e.g. it does not cause rusting of steel objects).
Sterilization (microbiology) - Wikipedia
Hot air oven - Wikipedia
master.on
New Member
Nice sterilization overview
wrapping described in page 39
wrapping described in page 39
Slammich
New Member
@master.on great info there. I def wrapped it before I...baked it.
