Method to Sterilize Media Bottles

[RealET]

I’ll keep in brief.

What I’m working with:
3 media bottles
3 compounds
1 bottle top filter
1 brake bleeder
Pressure cooker, distilled water

My goal:
I want the 3 different compounds in 3 differ media bottles. I’m planning to filter into one bottle, pour the next compound filter in the next bottle and so on.

My question:
When sterilizing all 3 media bottles with the pressure cooker, what should be done with the other media bottles when waiting for the current compound to finish filtering?

Leave it in the pressure cooker and keep the heat on until it’s time to filter the next ? Dry them all and then just leave them lying around? I’m concerned with contamination.

Better yet, how long can a media bottle be left around after sterilization?

 

[mp46]

What are you covering the openings with? People will often put foil over the opening if that foil stays in place then it'll stay sterile.

 

[master.on]

Pressure cookers take much longer (several hours) to fully sterilize than real autoclaves.

This study that a pressure cooker didn't achieve proper sterility after 24 HOURS
https://c.ymcdn.com/sites/www.adint...NewsArticles/Use_of_pressure_cookers_for_.pdf
So it's better to get a real autoclave

On the cheap, the media bottle can be left in a 1N Sodium hydroxide (lye) solution for 24 hours
then rinsed with sterile filtered 70% alcohol, and the alcohol is allowed to evaporate.
(Make sure the filter membrane is alcohol resistant).

 

[jJjburton]

Does this work for vials stoppers and aluminum tops. Rubbing alcohol, Boiling water, then dry heat over. Then asemble.

 

[Krampus]

Pressure cookers take much longer (several hours) to fully sterilize than real autoclaves.

This study that a pressure cooker didn't achieve proper sterility after 24 HOURS
https://c.ymcdn.com/sites/www.adint...NewsArticles/Use_of_pressure_cookers_for_.pdf
So it's better to get a real autoclave

On the cheap, the media bottle can be left in a 1N Sodium hydroxide (lye) solution for 24 hours
then rinsed with sterile filtered 70% alcohol, and the alcohol is allowed to evaporate.
(Make sure the filter membrane is alcohol resistant).

I couldn't get your link to load, do you have another? I'd like to see the study because I think this is an odd generalization, an autoclave is a high pressure pressure cooker. There are, of course, many variations of pressure cooker.

 

[captainmorgan]

Pressure cookers take much longer (several hours) to fully sterilize than real autoclaves.

This study that a pressure cooker didn't achieve proper sterility after 24 HOURS
https://c.ymcdn.com/sites/www.adint...NewsArticles/Use_of_pressure_cookers_for_.pdf
So it's better to get a real autoclave

On the cheap, the media bottle can be left in a 1N Sodium hydroxide (lye) solution for 24 hours
then rinsed with sterile filtered 70% alcohol, and the alcohol is allowed to evaporate.
(Make sure the filter membrane is alcohol resistant).

Thanks for the link to the study. I am doing some research since I have been out of the game for a while. Gonna try brewing up some 13 year old cyp powder and enanthate goo so I needed to refresh myself on DIY brewing. I have read the report a couple of times and it says they are sterilized (top of page 9) if you follow the guidelines on heat, pressure and time.
Is there something I am missing?
On the bottom of page 5 it gives you an idea of what could go wrong and what to look out for.
But anyone looking to use this method needs to read the report a few times to make sure they understand how much pressure, heat, water and time is needed. Thanks again for the link @master.on

 

[Krampus]

I got it to load this time.

This study that a pressure cooker didn't achieve proper sterility after 24 HOURS

Um, that's talking about the spore checking process, not the autoclave duration, and you left out the seven days.

"Failure to achieve these conditions was judged by positive growth of bacillus stearothermophilus in the “Spor Ampules” after 24 hours and seven days culture at 56⁰C."

"Direct exposure to saturated steam at 121⁰C for 10 minutes normally destroys all forms of microbial life although additional time must be allowed for the temperature to reach all surfaces of instrumentsbeing subjected to the sterilizing cycle. "

"Steam pressure of 103 kPa (15 psi) at 121⁰C can be achieved in all vessels included in the study without any modification to their components."

 

[Robfromga]

Amazon sells a product call alcalox. Spelling might be off, but it's used to was lab equipment. Powder in a 1/2 gallon cardboard box.

Wash with that
Then distilled rinse
And bake em at 400° for an hour.

You can add a 70% iso rinse in there if you'd like.

I've used that several times, very easy. The stuff will destroy your skin.

Using some npp now that was brewed a year ago using that wash/sterile system, all good.

 

[jstarks11]

Amazon sells a product call alcalox. Spelling might be off, but it's used to was lab equipment. Powder in a 1/2 gallon cardboard box.

Wash with that
Then distilled rinse
And bake em at 400° for an hour.

You can add a 70% iso rinse in there if you'd like.

I've used that several times, very easy. The stuff will destroy your skin.

Using some npp now that was brewed a year ago using that wash/sterile system, all good.

This is ^ correct. In order to sterilize things for the purposes of homebrewing etc it is much like when you sterilize the pin site by simply SWABBING with Isopropanol pads!!! I use a bleach/Ammonia water solution to thoroughly wash all lab glass including vials with vial brushes. then use a tub of methanol to rinse AFTER a regular water rinse. The methanol rinses off any minerals from your water rinse (magnesium, calcium, sodium, etc). NO OPEN FLAMES!!! Methanol is Flammable. Now, once you have them rinsed, put all things upside down on new aluminum on a tray and slide in a preheated oven 250F and bake for an hour. IF something lives through that that resides in your kitchen then you must have some of those extremophiles from out of volcano's or the superheated bacteria spew that comes out of some parts of the bottom of the ocean. IE you will have sterile gear. When you take your glass out of the oven all turned upside down, you use a bottle of CAVIWIPES like baby wipes but alcohol instead. and each time you flip a vial or bottle over to use you just wipe the rim that was laying on the foil with a qick swipe of the alcohol wipe then fill stopper cap or filter or whatever you do. And be confident you are sterile, unless you are working in a dusty construction site where people are sanding sheetrock etc... Otherwise you are nice and sterile at this point.