Multi Pin Fatigue - Mixing different Peps+HCG in same pin

[javelinmg]

Taking HCG and Retatrutide and added in BPC+TB+GHKCu(Glow Blend) for an injury and may even add HGH or one of the secretagogues at some point - but will probably hold off until done with the BPC because so many needles already.

I'm already pinning Test separately IM, and was pinning HCG SQ prior to adding Reta and Glow. Since adding these other peps, I'm pinning with quite a few different needles now.

Can I just draw HCG+BPC+TB+GHKCU+Reta and time it where I inject HCG/Reta the same day so I can at least reduce it to a single pin for SQ on those days? Or should I keep these separate and go through some days where I'm pinning with 4 different needles? Is there some guideline to follow which peps can be mixed and which can't?

 

[roofer22]

I feel your pain, at times my daily shots get up there. The only two I know mix well is bpc and tb-500. You can buy them mixed already. I don't know about the other stuff.

 

[Ateam2023]

i mix hgh, bpc157, tb500 with no problems, when i was running hcg i had that in the mix in one syringe

 

[Strongerhope]

i mix hgh, bpc157, tb500 with no problems, when i was running hcg i had that in the mix in one syringe

What about combining all of those into 1 vial? Ever try that? The bpc/tb4 i've combined but not hgh/hcg. not sure if that'd cause issues.

 

[Ghoul]

Oh yeah that's fine. Mix all the strands of amino acids you want. There's no chance of those adhering to each other at various. points. creating aggregates.

If you don't lose an ass cheek or immediately go into anaphylactic shock, you're 100% good to go.

These facts have been lab verified.

 

[bighunanballs]

I was doing this myself but as I listened to more ghoul research describing the aggregate formation I might stop. Often when mixing my HGH with other shit like my Reta or Cagri shots the solutions end up turning cloudy when mixed. I now fear I'm causing this very aggregation of peptides chains sticking together which in turn cause immunogenicity responses in the body

 

[MackVMT]

Taking HCG and Retatrutide and added in BPC+TB+GHKCu(Glow Blend) for an injury and may even add HGH or one of the secretagogues at some point - but will probably hold off until done with the BPC because so many needles already.

I'm already pinning Test separately IM, and was pinning HCG SQ prior to adding Reta and Glow. Since adding these other peps, I'm pinning with quite a few different needles now.

Can I just draw HCG+BPC+TB+GHKCU+Reta and time it where I inject HCG/Reta the same day so I can at least reduce it to a single pin for SQ on those days? Or should I keep these separate and go through some days where I'm pinning with 4 different needles? Is there some guideline to follow which peps can be mixed and which can't?

Should have no issues. I would think with all that stuff though, you would need two slin pins simply due to volume.

 

[javelinmg]

Should have no issues. I would think with all that stuff though, you would need two slin pins simply due to volume.

Well on days that I take reta hcg and glow that’s 3 slins, was looking to reduce this to at least 1. And occasionally an IM test pin. I guess I can live with that just seems like a lot.

So far seems like it’s best to just keep them separate pins in case of amino joining/adhesion, but will see what other opinions might chime in. In the meantime need to rotate to more spots I guess. Butt cheek fat, thigh fat, gut fat etc

 

[bighunanballs]

Well on days that I take reta hcg and glow that’s 3 slins, was looking to reduce this to at least 1. And occasionally an IM test pin. I guess I can live with that just seems like a lot.

So far seems like it’s best to just keep them separate pins in case of amino joining/adhesion, but will see what other opinions might chime in. In the meantime need to rotate to more spots I guess. Butt cheek fat, thigh fat, gut fat etc

I've mixed for years. I still mix all my oil based shit. I used to mix oil and water with no issues or so I thought. I think I'll start keeping all peptides separated out of coagulation/precipitate fears. My cocktails always turn cloudy when I mix them which is probably a bad sign I'm disrupting the peptides chains or at minimum altering the solubility of the excipients so that precipitates drop out of solution. I've never had any pain or pip but why spend all this money on drugs if we don't utilize best practices to get the most out of our dugs? I am a bit too lazy to filter like @Ghoul recommends but Ive started using more solvent with my peptides reconstitution protocol in hopes I would be lowering aggregate formation.

 

[Ghoul]

I've mixed for years. I still mix all my oil based shit. I used to mix oil and water with no issues or so I thought. I think I'll start keeping all peptides separated out of coagulation/precipitate fears. My cocktails always turn cloudy when I mix them which is probably a bad sign I'm disrupting the peptides chains or at minimum altering the solubility of the excipients so that precipitates drop out of solution. I've never had any pain or pip but why spend all this money on drugs if we don't utilize best practices to get the most out of our dugs? I am a bit too lazy to filter like @Ghoul recommends but Ive started using more solvent with my peptides reconstitution protocol in hopes I would be lowering aggregate formation.

FYI, a lot of assumptions are made by AAS users, understandably, paralleling steroids and peptides.

Peptides, once reconstituted, meaning the proteins at hydrated, do not *fall out of solution.*, ie, "crash".

When a previously clear peptide solution goes cloudy, it means aggregates in the visibile range, larger than >100um, have formed.

Dimer is the smallest form of aggregate (most don't know what dimer is, other than you don't want it in your hgh), while visible particulates represent the largest.

The larger the aggregate, the more of a health risk it represents for various reasons.

Above 20um is considered high risk,

Multiple peptides form unique aggregates, and the immune system response can't be predicted. If the shape just happens to be similar enough to some natural, important peptide, of which there are thousands, the immune system can learn that shape is the "enemy", start attacking them, and cause undetected damage, especially in the brain, where deformed and missing peptides are closely associated with neurodegeneration. IE, a person who's developed severe cognitive dysfunction typically has significant malformed peptides in their body.

Filter your peptides to eliminate the largest aggregates. All peptides can easily pass through a .2um PES filter.

You can filter the entire reconstituted vial at one time into a sterile vial, which will help significantly, However, since aggregates take time to form, once reconstituted, ideally filter as close to administration as possible.

Another compromise would be to use vials with small enough amounts of active ingredient they get used up soon after reconstitution.

And of course, use the same dilution ratio pharma uses, or more. The more
concentrate the solution, the more
aggregation takes place.

 

[bighunanballs]

FYI, a lot of assumptions are made by AAS users, understandably, paralleling steroids and peptides.

Peptides, once reconstituted, meaning the proteins at hydrated, do not *fall out of solution.*, ie, "crash".

When a previously clear peptide solution goes cloudy, it means aggregates in the visibile range, larger than >100um, have formed.

Dimer is the smallest form of aggregate (most don't know what dimer is, other than you don't want it in your hgh), while visible particulates represent the largest.

The larger the aggregate, the more of a health risk it represents for various reasons.

Above 20um is considered high risk,

Multiple peptides form unique aggregates, and the immune system response can't be predicted. If the shape just happens to be similar enough to some natural, important peptide, of which there are thousands, the immune system can learn that shape is the "enemy", start attacking them, and cause undetected damage, especially in the brain, where deformed and missing peptides are closely associated with neurodegeneration. IE, a person who's developed severe cognitive dysfunction typically has significant malformed peptides in their body.

Filter your peptides to eliminate the largest aggregates. All peptides can easily pass through a .2um PES filter.

You can filter the entire reconstituted vial at one time into a sterile vial, which will help significantly, However, since aggregates take time to form, once reconstituted, ideally filter as close to administration as possible.

Another compromise would be to use vials with small enough amounts of active ingredient they get used up soon after reconstitution.

And of course, use the same dilution ratio pharma uses, or more. The more
concentrate the solution, the more
aggregation takes place.

Great information, one of these days I might beer myself the strength to filter my peptides. For now my lazy ass will stick to higher solvent reconstitution process and non mixing of aqueous solutions to keep shit from going cloudy.

On another note it's interesting that we saw so many rashes of cloudy HGH even when it was all testing around 96% and very low dimer. I'm wondering now if the cloudy HGH is from visible aggregates or from shitty excipient recipes

 

[Ghoul]

Great information, one of these days I might beer myself the strength to filter my peptides. For now my lazy ass will stick to higher solvent reconstitution process and non mixing of aqueous solutions to keep shit from going cloudy.

On another note it's interesting that we saw so many rashes of cloudy HGH even when it was all testing around 96% and very low dimer. I'm wondering now if the cloudy HGH is from visible aggregates or from shitty excipient recipes

Aggregation is almost entirely determined by the conditions of the reconstituted peptide. The PH, the presence or lack thereof of anti aggregation excipients, crap in the vial like silicone etc.

But dimer, and other contaminants produced and not filtered out during peptide manufacturing do act as "seeds" promoting aggregation upon reconstitution. Dimer (two monomers, ie the 'correct' peptide chain, fused together into the smallest aggregate) are formed while the peptide is still liquid, before its freeze dried.

The pharma brands of hgh with the fewest sides reported to the FDA have no significant anti-aggregation excipients, are reconstituted with sterile water not BAC, and used immediately.

I think they have the fewest sides because there's simply no time for aggregates to form.

 

[Ghoul]

If you want some real nightmare fuel, while most aggregates are just amorphous shaped trash, wasting good peptide, but otherwise just cause a little inflammation and get destroyed by the immune system, some that slip through the cracks, in just the wrong shape, can act as a "mold", causing other peptides in your body (again, especially the brain), to misform, starting a chain reaction that can last for decades before symptoms are noticeable.

FYI, oligomers are the aggregation stage after dimer.

Protein misfolding, aggregation, and conformational strains in neurodegenerative diseases - Nature Neuroscience

A newly recognized process in neurodegenerative disease is accumulation of misfolded protein aggregates that self-replicate to spread damage between cells and tissues. This process has implications in designing strategies for treatment and diagnosis.

www.nature.com

So as you start to drool while the person changing your diaper transforms into looking like a demon from hell, but you forgot how to use words so you can't say a prayer to scare it off, with the last little functioning bit of consciousness you can comfort yourself with the thought "at least I saved myself from having to inject more than once".

-------

If this is all too abstract, here's some GLP-1 intentionally reconstituted at the wrong PH, left in a high heat environment, to quickly grow large aggregates. The fiber shaped aggregates are known as fibrils, and seem to be particularly harmful, vs globules.

https://royalsocietypublishing.org/doi/pdf/10.1098/rsfs.2017.0030

 

[Ateam2023]

What about combining all of those into 1 vial? Ever try that? The bpc/tb4 i've combined but not hgh/hcg. not sure if that'd cause issues.

no thanks

 

[Strongerhope]

If you want some real nightmare fuel, while most aggregates are just amorphous shaped trash, wasting good peptide, but otherwise just cause a little inflammation and get destroyed by the immune system, some that slip through the cracks, in just the wrong shape, can act as a "mold", causing other peptides in your body (again, especially the brain), to misform, starting a chain reaction that can last for decades before symptoms are noticeable.

FYI, oligomers are the aggregation stage after dimer.

View attachment 306698

Protein misfolding, aggregation, and conformational strains in neurodegenerative diseases - Nature Neuroscience

A newly recognized process in neurodegenerative disease is accumulation of misfolded protein aggregates that self-replicate to spread damage between cells and tissues. This process has implications in designing strategies for treatment and diagnosis.

www.nature.com

So as you start to drool while the person changing your diaper transforms into looking like a demon from hell, but you forgot how to use words so you can't say a prayer to scare it off, with the last little functioning bit of consciousness you can comfort yourself with the thought "at least I saved myself from having to inject more than once".

-------

If this is all too abstract, here's some GLP-1 intentionally reconstituted at the wrong PH, left in a high heat environment, to quickly grow large aggregates. The fiber shaped aggregates are known as fibrils, and seem to be particularly harmful, vs globules.

View attachment 306700

https://royalsocietypublishing.org/doi/pdf/10.1098/rsfs.2017.0030

How do we prevent this?

Does this only happen in severely degraded situations ?

Or is it just the risk of using any peptides?