master.on
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Sodium hydroxide, also called lye or caustic soda
Sodium hydroxide - Wikipedia
Potential for chemical sterilization of prions
Prions are highly resistant to chemical sterilization. Treatment with aldehydes such as formaldehyde have actually been shown to increase prion resistance. Hydrogen peroxide (3%) for one hour was shown to be ineffective, providing less than 3 logs (10−3) reduction in contamination. Iodine, formaldehyde, glutaraldehyde and peracetic acid also fail this test (one hour treatment). Only chlorine, phenolic compounds, guanidinium thiocyanate, and sodium hydroxide (NaOH) reduce prion levels by more than 4 logs; chlorine (too corrosive to use on certain objects) and NaOH are the most consistent. Many studies have shown the effectiveness of sodium hydroxide.
Sterilization (microbiology) - Wikipedia
Not only it literally digests bacteria, viruses, fungi
it destroys small spores, viruses and bacterial proteins that usually pass trough 0.22 filters
destroys prions that even pass through 15 NANOmeter ultrafiltration (and ALL MICROmeter filters)
Infectious prion protein in the filtrate even after 15 nm filtration. - PubMed - NCBI
- Rinsing in Sodium Hydroxide for 1 hour at room temperature seems to be enough
But why not leave it overnight, as research shows the longer the better.
- Adding Ethyl alcohol (to 20%) makes it better/faster on spores.
- 0.1 M (4 grams Sodium hydroxide per liter) works fine, but 0.5 M (20 grams per liter) or 1 M (4 g per liter) works better/faster on endotoxins.
- Sodium chloride (plain table salt) makes it better, and it's dirt cheap.
Full study here:
https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1338541738309/litdoc18112457_20161012164839.pdf (https://www.gelifesciences.com/gehcls_images/GELS/Related Content/Files/1338541738309/litdoc18112457_20161012164839.pdf)
It even dissolves cattle bones, skin and flesh to make gelatin
The swelling of collagen in alkaline solutions. 1. Swelling in solutions of sodium hydroxide
Even criminals use it to get rid of corpses
Sodium hydroxide - Wikipedia
Butyl rubber seems to withstand Sodium hydroxide
Rubber Chemical Resistance, Rubber Chemical Compatibility, Page 5 - Mykin Inc
so it's safe for vial stoppers.
After immersing your vials/stoppers/media bottles
rinse with distilled water, preferably sterile water.
Rinse very thoroughly, but even if a TINY amount of Sodium Hydroxide remains. it's safe as it's used to adjust pH in many injectable meds.
Sodium Hydroxide (Inactive Ingredient) - Drugs.com
Of course, don't attempt to sterilize your raws, oil, BA or BB with it.
Sodium hydroxide - Wikipedia
Potential for chemical sterilization of prions
Prions are highly resistant to chemical sterilization. Treatment with aldehydes such as formaldehyde have actually been shown to increase prion resistance. Hydrogen peroxide (3%) for one hour was shown to be ineffective, providing less than 3 logs (10−3) reduction in contamination. Iodine, formaldehyde, glutaraldehyde and peracetic acid also fail this test (one hour treatment). Only chlorine, phenolic compounds, guanidinium thiocyanate, and sodium hydroxide (NaOH) reduce prion levels by more than 4 logs; chlorine (too corrosive to use on certain objects) and NaOH are the most consistent. Many studies have shown the effectiveness of sodium hydroxide.
Sterilization (microbiology) - Wikipedia
Not only it literally digests bacteria, viruses, fungi
it destroys small spores, viruses and bacterial proteins that usually pass trough 0.22 filters
destroys prions that even pass through 15 NANOmeter ultrafiltration (and ALL MICROmeter filters)
Infectious prion protein in the filtrate even after 15 nm filtration. - PubMed - NCBI
- Rinsing in Sodium Hydroxide for 1 hour at room temperature seems to be enough
But why not leave it overnight, as research shows the longer the better.
- Adding Ethyl alcohol (to 20%) makes it better/faster on spores.
- 0.1 M (4 grams Sodium hydroxide per liter) works fine, but 0.5 M (20 grams per liter) or 1 M (4 g per liter) works better/faster on endotoxins.
- Sodium chloride (plain table salt) makes it better, and it's dirt cheap.
Full study here:
https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1338541738309/litdoc18112457_20161012164839.pdf (https://www.gelifesciences.com/gehcls_images/GELS/Related Content/Files/1338541738309/litdoc18112457_20161012164839.pdf)
It even dissolves cattle bones, skin and flesh to make gelatin
The swelling of collagen in alkaline solutions. 1. Swelling in solutions of sodium hydroxide
Even criminals use it to get rid of corpses
Sodium hydroxide - Wikipedia
Butyl rubber seems to withstand Sodium hydroxide
Rubber Chemical Resistance, Rubber Chemical Compatibility, Page 5 - Mykin Inc
so it's safe for vial stoppers.
After immersing your vials/stoppers/media bottles
rinse with distilled water, preferably sterile water.
Rinse very thoroughly, but even if a TINY amount of Sodium Hydroxide remains. it's safe as it's used to adjust pH in many injectable meds.
Sodium Hydroxide (Inactive Ingredient) - Drugs.com
Of course, don't attempt to sterilize your raws, oil, BA or BB with it.