So I've been playing around with different home brews just for the fuck of it and had one super interesting result.
This was an experiment to try:
* 100mL total, USP grade Grapeseed Oil with no hexane.
* 250mg/mL Test Cyp
* 0% BB (yeah, yeah, I know, it's an experiment, okay?)
* 1% BA
Anyways, fucking disaster of a brew overall.
Good parts:
1) So I've got this super fancy vacuum filtration setup that actually worked fucking GREAT. It was the one thing that worked great, btw. Basically I got some NEST 343101 PVDF filter bottles. They're plastic, so they can't handle a "full vacuum" or they crack and leak, so I got a needle valve, a pressure gauge, and a 4-way NPT thread cross so I can control the vacuum pressure with the needle valve. Found that 20 inches of mercury vacuum doesn't crack the plastic filter bottle but still filters at a decent 24 minutes per 100mL (0% BB, remember, so it's def thicker).
Anyways I like this bottle because it comes fully sterilized which makes it easier to not fuck around with.
2) I didn't overheat or burn the oil/test. (max temp 82 C for a split second, otherwise all less than 80C)
The disaster parts:
* Glass magnetic stir bar broke when I dropped it 3 inches. Guess I'll stick with fluorinated plastic coating next time -- they're cheaper too and pretty indestructible but with glass you don't have to worry about any solvents at all ever.
* Erlenmeyer Flask was too big (250mL for 100mL batch) so the temperature prob for my magnetic stirrer / hot plate couldn't reach all the way in. Luckily I had a backup thermocouple connected to a super expensive multimeter so I could monitor oil temperature.
* Really should have used nitrile gloves.
* Need some kind of funnel for pouring 25g of test cyp into the oil. For some reason decided to put it into 100ml graduated cylinder, it took up 75mL of it and then I poured warm oil on top of it to try to dissolve it aaaaand....giant mess. Everything clogged up. Hilarious and stupid. Next time, funnel into warm Erlenmeyer flask.
* Spilled random amount of oil and raws powder all over the countertop/floor. Probably not much, but no clue.
* Raws powder residue stuck to the side of all the glassware. No idea how much.
* Needed a mixing rod, didn't have one, ended up using stainless steel chopstick that I hadn't fully sterilized like the rest of my equipment (but only pre-filtering so whatever I guess)
Well, I hope that was sufficiently horrifying/entertaining. Now for the weird part I actually want feedback on:
* I heated everything to 80C, it was perfectly clear and homogenous, nice yellowish color. Lots of stirring. Definitely 100mL of homogenous, stirred brew.
* Then I filtered all 100mL in one batch for like 40 minutes.
* I filled two 50mL pre-sealed sterile vials, labeled them and put them in my closet. The first vial was clear and looked normal. Second vial was cloudy immediately after filling it.
* Four hours later, the first vial looks normal, the second vial is....completely solid like refrigerated butter.
How the fuck is the first vial and second vial so different when they came from the same perfectly mixed and filtered bottle?
This was an experiment to try:
* 100mL total, USP grade Grapeseed Oil with no hexane.
* 250mg/mL Test Cyp
* 0% BB (yeah, yeah, I know, it's an experiment, okay?)
* 1% BA
Anyways, fucking disaster of a brew overall.
Good parts:
1) So I've got this super fancy vacuum filtration setup that actually worked fucking GREAT. It was the one thing that worked great, btw. Basically I got some NEST 343101 PVDF filter bottles. They're plastic, so they can't handle a "full vacuum" or they crack and leak, so I got a needle valve, a pressure gauge, and a 4-way NPT thread cross so I can control the vacuum pressure with the needle valve. Found that 20 inches of mercury vacuum doesn't crack the plastic filter bottle but still filters at a decent 24 minutes per 100mL (0% BB, remember, so it's def thicker).
Anyways I like this bottle because it comes fully sterilized which makes it easier to not fuck around with.
2) I didn't overheat or burn the oil/test. (max temp 82 C for a split second, otherwise all less than 80C)
The disaster parts:
* Glass magnetic stir bar broke when I dropped it 3 inches. Guess I'll stick with fluorinated plastic coating next time -- they're cheaper too and pretty indestructible but with glass you don't have to worry about any solvents at all ever.
* Erlenmeyer Flask was too big (250mL for 100mL batch) so the temperature prob for my magnetic stirrer / hot plate couldn't reach all the way in. Luckily I had a backup thermocouple connected to a super expensive multimeter so I could monitor oil temperature.
* Really should have used nitrile gloves.
* Need some kind of funnel for pouring 25g of test cyp into the oil. For some reason decided to put it into 100ml graduated cylinder, it took up 75mL of it and then I poured warm oil on top of it to try to dissolve it aaaaand....giant mess. Everything clogged up. Hilarious and stupid. Next time, funnel into warm Erlenmeyer flask.
* Spilled random amount of oil and raws powder all over the countertop/floor. Probably not much, but no clue.
* Raws powder residue stuck to the side of all the glassware. No idea how much.
* Needed a mixing rod, didn't have one, ended up using stainless steel chopstick that I hadn't fully sterilized like the rest of my equipment (but only pre-filtering so whatever I guess)
Well, I hope that was sufficiently horrifying/entertaining. Now for the weird part I actually want feedback on:
* I heated everything to 80C, it was perfectly clear and homogenous, nice yellowish color. Lots of stirring. Definitely 100mL of homogenous, stirred brew.
* Then I filtered all 100mL in one batch for like 40 minutes.
* I filled two 50mL pre-sealed sterile vials, labeled them and put them in my closet. The first vial was clear and looked normal. Second vial was cloudy immediately after filling it.
* Four hours later, the first vial looks normal, the second vial is....completely solid like refrigerated butter.
How the fuck is the first vial and second vial so different when they came from the same perfectly mixed and filtered bottle?
