Hi everyone- I've made small batches with success, now I'm looking to make larger batches for myself so I only have to make them like once a year. I did some research and put together a quick guide to make it easy for me. I took some info from searching this forum combined with my own knowledge. I hoping to get some insight to perfect this guide, which can bring value not only to myself but also everyone here. What am I missing or could be improved on ? Also put some questions within the steps of things I'm unsure of. Any advice would be greatly appretiated and I'm willing to offer any help I can as well!
Step 1:
Sterilize Media bottle- Wash with hot water & 10% bleach- Then wash with hot water and dish soap- Dry it oven at 400 for 30minutes or until completely dry
Sterilize Beaker- Wash with hot water & 10% bleach- Then wash with hot water and dish soap- Dry it oven at 400 for 30minutes or until completely dry
Sterilize 10ml vials – When using many vials to fill, washing them individually doesn’t seem like the most effective way. What is the best way to sterilize 10ml open vials?
Sterilize bottle top dispenser- ?
Sterilize Heat plate temp sensor and magnet- ?
Beaker used- https://www.amazon.com/Glass-Beaker-Low-Form-2000ml/dp/B07MZCHPHR/ref=sr_1_4?crid=2NJNRYN4T0IOV&keywords=2000ml+beaker&qid=1677598276&sprefix=2000ml+beaker%2Caps%2C170&sr=8-4
Beaker used- https://www.amazon.com/Glass-Beaker-Low-Form-2000ml/dp/B07MZCHPHR/ref=sr_1_4?crid=2NJNRYN4T0IOV&keywords=2000ml+beaker&qid=1677598276&sprefix=2000ml+beaker%2Caps%2C170&sr=8-4
10ml vials used- RLS 10ml Tubular Amber Glass Serum Vials (Qty. 1)
Step 2: Weigh powder using digital scale
Use this to form calculation- https://www.aasraw.com/steroid-calculator/
Scale used - 3000g x 0.1g accuracy, Digital Table Top Scale (Qty. 1)
Step 3: Add powder, BA, BB and grapeseed oil into the Beaker.
BA- Benzyl Alcohol, Anhydrous, 99.9% Pure, USP 500mL
BB- Buy Benzyl Benzoate USP Grade, Ultra Pure, 500 mL
Oil- Buy Grapeseed Oil by Vale of Eden, 1 Gallon (Approx. 4 Liters)
Beaker- https://www.amazon.com/Glass-Beaker-Low-Form-2000ml/dp/B07MZCHPHR/ref=sr_1_4?crid=2NJNRYN4T0IOV&keywords=2000ml+beaker&qid=1677598276&sprefix=2000ml+beaker%2Caps%2C170&sr=8-4
Step 4: Place Beaker on Hot plate/Magnetic Stirrer
Heat to 250f - Keep an eye on the powder and watch as it dissolves until it gets close to clear. A clear solution means that the mixture is now melted.
All this contributes to dissolving the powder entirely into the solvents. Move to the next step when no powder is visible.
Hot Plate/ Magnetic Stirrer used- https://www.amazon.com/Hotplate-Magnetic-100-1500rpm-Warranty-Stirring/dp/B09FPZMKKF/ref=sr_1_1_sspa?crid=3TPQDKULSGAMG&keywords=Hot%2BPlate%2Bw%2BMagnetic%2Bstirrer&qid=1677595076&sprefix=hot%2Bplate%2Bw%2Bmagnetic%2Bstirrer%2Caps%2C481&sr=8-1-spons&ufe=app_do%3Aamzn1.fos.e3dc0fa2-4ee8-4881-a97d-5992ecbdb227&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUFKTTJWMDJLR1BVWUcmZW5jcnlwdGVkSWQ9QTAyMTc4MjMxRFgxSEZVVDEyVkFPJmVuY3J5cHRlZEFkSWQ9QTA2MTM2NTczVFNCV0VTSEhYUVFaJndpZGdldE5hbWU9c3BfYXRmJmFjdGlvbj1jbGlja1JlZGlyZWN0JmRvTm90TG9nQ2xpY2s9dHJ1ZQ&th=1
Step 5: Filtering
Filter while solution is warm (but not hot) Around what temp would you recommend for this stage of filtration ?
Place Whatman filter on top of sterilized media bottle
Pour from beaker onto Whatman filter
Connect pump to Whatman filter
Filter- 500ml Whatman Zapcap CR, PTFE, 45um, 33/45mm neck, Qty. 1
Pump- https://www.amazon.com/gp/product/B08GWGCCL2/ref=ox_sc_act_title_1?smid=AK611BWOCS7MA&psc=1
Step 6: Bottling and crimping
Once filtration is complete- Remove Filter & Place bottle top dispenser on top of media bottle
Pour 10ml into each 10ml vial from media bottle with bottle top dispenser
Place rubber stoppers on top of each vial
Place flip off caps on top of each vial- crimp with crimper
Bottle top dispenser- 1-10mL Bottle Top Dispenser | U.S. Plastic Corp.
Step 1:
Sterilize Media bottle- Wash with hot water & 10% bleach- Then wash with hot water and dish soap- Dry it oven at 400 for 30minutes or until completely dry
Sterilize Beaker- Wash with hot water & 10% bleach- Then wash with hot water and dish soap- Dry it oven at 400 for 30minutes or until completely dry
Sterilize 10ml vials – When using many vials to fill, washing them individually doesn’t seem like the most effective way. What is the best way to sterilize 10ml open vials?
Sterilize bottle top dispenser- ?
Sterilize Heat plate temp sensor and magnet- ?
Beaker used- https://www.amazon.com/Glass-Beaker-Low-Form-2000ml/dp/B07MZCHPHR/ref=sr_1_4?crid=2NJNRYN4T0IOV&keywords=2000ml+beaker&qid=1677598276&sprefix=2000ml+beaker%2Caps%2C170&sr=8-4
Beaker used- https://www.amazon.com/Glass-Beaker-Low-Form-2000ml/dp/B07MZCHPHR/ref=sr_1_4?crid=2NJNRYN4T0IOV&keywords=2000ml+beaker&qid=1677598276&sprefix=2000ml+beaker%2Caps%2C170&sr=8-4
10ml vials used- RLS 10ml Tubular Amber Glass Serum Vials (Qty. 1)
Step 2: Weigh powder using digital scale
Use this to form calculation- https://www.aasraw.com/steroid-calculator/
Scale used - 3000g x 0.1g accuracy, Digital Table Top Scale (Qty. 1)
Step 3: Add powder, BA, BB and grapeseed oil into the Beaker.
BA- Benzyl Alcohol, Anhydrous, 99.9% Pure, USP 500mL
BB- Buy Benzyl Benzoate USP Grade, Ultra Pure, 500 mL
Oil- Buy Grapeseed Oil by Vale of Eden, 1 Gallon (Approx. 4 Liters)
Beaker- https://www.amazon.com/Glass-Beaker-Low-Form-2000ml/dp/B07MZCHPHR/ref=sr_1_4?crid=2NJNRYN4T0IOV&keywords=2000ml+beaker&qid=1677598276&sprefix=2000ml+beaker%2Caps%2C170&sr=8-4
Step 4: Place Beaker on Hot plate/Magnetic Stirrer
Heat to 250f - Keep an eye on the powder and watch as it dissolves until it gets close to clear. A clear solution means that the mixture is now melted.
All this contributes to dissolving the powder entirely into the solvents. Move to the next step when no powder is visible.
Hot Plate/ Magnetic Stirrer used- https://www.amazon.com/Hotplate-Magnetic-100-1500rpm-Warranty-Stirring/dp/B09FPZMKKF/ref=sr_1_1_sspa?crid=3TPQDKULSGAMG&keywords=Hot%2BPlate%2Bw%2BMagnetic%2Bstirrer&qid=1677595076&sprefix=hot%2Bplate%2Bw%2Bmagnetic%2Bstirrer%2Caps%2C481&sr=8-1-spons&ufe=app_do%3Aamzn1.fos.e3dc0fa2-4ee8-4881-a97d-5992ecbdb227&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUFKTTJWMDJLR1BVWUcmZW5jcnlwdGVkSWQ9QTAyMTc4MjMxRFgxSEZVVDEyVkFPJmVuY3J5cHRlZEFkSWQ9QTA2MTM2NTczVFNCV0VTSEhYUVFaJndpZGdldE5hbWU9c3BfYXRmJmFjdGlvbj1jbGlja1JlZGlyZWN0JmRvTm90TG9nQ2xpY2s9dHJ1ZQ&th=1
Step 5: Filtering
Filter while solution is warm (but not hot) Around what temp would you recommend for this stage of filtration ?
Place Whatman filter on top of sterilized media bottle
Pour from beaker onto Whatman filter
Connect pump to Whatman filter
Filter- 500ml Whatman Zapcap CR, PTFE, 45um, 33/45mm neck, Qty. 1
Pump- https://www.amazon.com/gp/product/B08GWGCCL2/ref=ox_sc_act_title_1?smid=AK611BWOCS7MA&psc=1
Step 6: Bottling and crimping
Once filtration is complete- Remove Filter & Place bottle top dispenser on top of media bottle
Pour 10ml into each 10ml vial from media bottle with bottle top dispenser
Place rubber stoppers on top of each vial
Place flip off caps on top of each vial- crimp with crimper
Bottle top dispenser- 1-10mL Bottle Top Dispenser | U.S. Plastic Corp.