bottle top filters

[kap73]

so I'm about to invest in some bottle top filters. i have a few questions on the use of them first. ok so you filter the oil into a media bottle and then from there u inject individually to each vial.

1. how do you keep in sterile from after the point of filtering into a media bottle if your exposing it to air to draw out of?

2. do you guys just use a glass media bottle with screw top? after each time just wash it out with BA then pressure cook it and reuse?

3. what is the best method for sterilising vials? do you wash with distilled water then rinse with BA then let dry and put foil over the top then pressure cook for 45min?

4. do you the inject oil thru the foil on top? bath stopper in BA then remove foil and place stopper on??

5. what bottle top filters do your recommend? looking at these brand atm 'corning' 'naglene' and 'what man'


do this sound correct

sorry for questions but i think i have it sussed just need a few things straightened out

 

[Docd187123]

1) they make rubber septums I think they're called for the glass media jars. It's like a big piece of rubber you put over the top and a special cap screws it in place. It's reusable and you just like through with a needle. The other choice is to just draw your oil into a large syringe or two immediately after filtering. Yes it has some risk associated with it but brewing in your home in general does.

2) you don't pressure cook anything that's open. If you want to reuse the media jar or sterilize it it's the same process as for open vials.

3) to sterilize vials: wash with detergent and hot water. Not soap but detergent or lab glass cleaner. Rinse 6 with water 6x. You can use tap water but the final rinse use distilled. Put it on a cookie tray and in the oven at 500deg F for 1hr. You now have 'sterile' vials. For the butyl stoppers, soak them in isopropyl alcohol for ~15min then bake in the over at 200deg F until the alcohol has evaporated. You don't pressure cook open vials bc they'll just be contaminated before you use them.

4) fill the open vial wih desired amount of oil. Place sterile stopper on the top than the flip top cap. Crimp and done.

5) heard too many bad things about millipore and Nalgene so I personally stay away. I used whatman ZapCap-CR. It's not sterile but pressure cooking the vial afterwards takes care of that. They have sterile whatman zapcaps but not the cr version.

 

[kap73]

thanks for your reply mate, that makes sense.

so you do not put foil over your vials when they are in the oven?

ok i will look into these what man ZapCap-CR's. thanks mate

 

[Notits]

http://www.medical-and-lab-supplies.com/lab-glassware/media-bottles/caps.html

 

[kap73]

http://www.medical-and-lab-supplies.com/lab-glassware/media-bottles/caps.html

good man. i will have a look, thank you

 

[kap73]

1) they make rubber septums I think they're called for the glass media jars. It's like a big piece of rubber you put over the top and a special cap screws it in place. It's reusable and you just like through with a needle. The other choice is to just draw your oil into a large syringe or two immediately after filtering. Yes it has some risk associated with it but brewing in your home in general does.

2) you don't pressure cook anything that's open. If you want to reuse the media jar or sterilize it it's the same process as for open vials.

3) to sterilize vials: wash with detergent and hot water. Not soap but detergent or lab glass cleaner. Rinse 6 with water 6x. You can use tap water but the final rinse use distilled. Put it on a cookie tray and in the oven at 500deg F for 1hr. You now have 'sterile' vials. For the butyl stoppers, soak them in isopropyl alcohol for ~15min then bake in the over at 200deg F until the alcohol has evaporated. You don't pressure cook open vials bc they'll just be contaminated before you use them.

4) fill the open vial wih desired amount of oil. Place sterile stopper on the top than the flip top cap. Crimp and done.

5) heard too many bad things about millipore and Nalgene so I personally stay away. I used whatman ZapCap-CR. It's not sterile but pressure cooking the vial afterwards takes care of that. They have sterile whatman zapcaps but not the cr version.

http://www.medical-and-lab-supplies.com/500ml-whatman-zapcap-33-45mm-fitting-neck-0-22-micron.html

so doc im guessing these are the sterile ones?? as they don't have CR on the title

 

[Docd187123]

thanks for your reply mate, that makes sense.

so you do not put foil over your vials when they are in the oven?

ok i will look into these what man ZapCap-CR's. thanks mate

I do not put foil over the vials. It might be easier for you to just buy sterile vials. It's a decision you'll have to make.

http://www.medical-and-lab-supplies.com/500ml-whatman-zapcap-33-45mm-fitting-neck-0-22-micron.html

so doc im guessing these are the sterile ones?? as they don't have CR on the title

I believe those to be the CRs. I think I bought the same ones. It would normally say sterile somewhere in the description of they are sterile. I pressure cook my oil after so it's not as big of a deal as if I didn't go through the process.

 

[kap73]

thank you for your advice and i like the sound of it so i will follow those steps. much appreciated

 

[MANWHORE]

1. how do you keep it sterile from after the point of filtering into a media bottle if your exposing it to air?

Air is sterile..
Unless your lab is very dusty,
nothing is floating into your vial

M

 

[kap73]

Air is sterile..
Unless your lab is very dusty,
nothing is floating into your vial

M

yeah i did kinda gather that but i guess i thought i'd best ask. cheers for your patience every one. I've read up on this stuff myself but sometimes i just want to run it by other people

 

[MANWHORE]

I filter straight into my vials..

M

 

[drksanctuary]

I do not put foil over the vials. It might be easier for you to just buy sterile vials. It's a decision you'll have to make.



I believe those to be the CRs. I think I bought the same ones. It would normally say sterile somewhere in the description of they are sterile. I pressure cook my oil after so it's not as big of a deal as if I didn't go through the process.

So, if I use a zapcap-cr for filtering which is non sterile and then use syringes to transfer to sterile sealed vials, pressure cooking them would make it completely sterile?

How long and at what psi would it go for?

Is there a risk of vial explosion or overheating the oil and messing up the brew?

Thanks for all the help.

 

[Gramps]

Air is sterile..
Unless your lab is very dusty,
nothing is floating into your vial

M

Not unless you have a "clean room". Air can be full of uglies. A sneeze or a cough can stay longer than you'd like. Other nasties can be blown in from anywhere without your knowledge. No, it's not likely a real biohazard, but let's not get too comfortable believing the air around us is safe to inject without proper care.

 

[Docd187123]

So, if I use a zapcap-cr for filtering which is non sterile and then use syringes to transfer to sterile sealed vials, pressure cooking them would make it completely sterile?

How long and at what psi would it go for?

Is there a risk of vial explosion or overheating the oil and messing up the brew?

Thanks for all the help.

I haven't been pressure cooking lately. I've just been filtering as that is what is important.

 

[MANWHORE]

Not unless you have a "clean room". Air can be full of uglies. A sneeze or a cough can stay longer than you'd like. Other nasties can be blown in from anywhere without your knowledge. No, it's not likely a real biohazard, but let's not get too comfortable believing the air around us is safe to inject without proper care.

You're over thinking it trust me brother I've been at this a long time and not only that but unlike other brewers I've had ALOT of people use my brews and never had an issue ...
Well not until meso shills and trolls came into the picture lol

 

[Gramps]

You're over thinking it trust me brother I've been at this a long time and not only that but unlike other brewers I've had ALOT of people use my brews and never had an issue ...
Well not until meso shills and trolls came into the picture lol

Nope, not overthinking it at all. And don't mistake me for crying "wolf" as well. There's a member here who's used a needle out of an ashtray - it doesn't mean there's no danger, luck was just on his side. There really can be nasty shit in the air whether you've been sick or not. I don't want anyone to read this and think it's fine to go and brew without taking precautions, since there are some nasty critters can even make it through a .22 filter. Slamming a single bullet in a revolver and pointing it at your head gives you a 5 outta 6 chance of having no issues at all. Don't push your luck, or at least if you do, don't tell others it's the right thing to do. It's not.

 

[MANWHORE]

 

[Big_paul]

Nope, not overthinking it at all. And don't mistake me for crying "wolf" as well. There's a member here who's used a needle out of an ashtray - it doesn't mean there's no danger, luck was just on his side. There really can be nasty shit in the air whether you've been sick or not. I don't want anyone to read this and think it's fine to go and brew without taking precautions, since there are some nasty critters can even make it through a .22 filter. Slamming a single bullet in a revolver and pointing it at your head gives you a 5 outta 6 chance of having no issues at all. Don't push your luck, or at least if you do, don't tell others it's the right thing to do. It's not.

Who the fuck used a needle out of an ashtray?

If you are filtering gear through a 22.5 into a sealed sterile vile, you are g2g. I take clean empty vials out and stoppers out of heat, place stoppers into the vials, so there is no chance of contamination, and cap it after I filtered gear into the vial.I have never had any irritation or swelling. Never.

 

[Gramps]

Nothing to see here... This simply caught my attention when a claim was made that air was sterile. Filtering stops most things, but to claim air is sterile leads to a false sense of security to those who simply may not know any different.

The ashtray? It's an A.M. here who had a bad drug habit and has since gone clean. I believe he was shooting H, but I may be mistaken as it's been a while. It's been posted public but he's a good dude who doesn't need me dragging his name into it. It's just an example of people doing dumb shit and being lucky. He admits that.

 

[Steezy]

As for #3.
When you put the vials on a cookie tray do you put them open end up or open end down on the cookie tray while baking? Like upright or upside down while baking?

3) to sterilize vials: wash with detergent and hot water. Not soap but detergent or lab glass cleaner. Rinse 6 with water 6x. You can use tap water but the final rinse use distilled. Put it on a cookie tray and in the oven at 500deg F for 1hr. You now have 'sterile' vials. For the butyl stoppers, soak them in isopropyl alcohol for ~15min then bake in the over at 200deg F until the alcohol has evaporated. You don't pressure cook open vials bc they'll just be contaminated before you use them.