nylon membrane filters 0.22

[Hendrix11]

I have bought some of these for a filter assembly set up I have bought.

My question is about sterility. They don't say sterile on them, and I'm guessing I don't want to be putting my fingers all over them?

How do I use these, stupid question but I don't have to autoclave first or anything do I?

Only ever used the pre packed sterile filter units so haven't got a clue???

Cheers in advance

 

[Docd187123]

I have bought some of these for a filter assembly set up I have bought. View attachment 23096

My question is about sterility. They don't say sterile on them, and I'm guessing I don't want to be putting my fingers all over them?

How do I use these, stupid question but I don't have to autoclave first or anything do I?

Only ever used the pre packed sterile filter units so haven't got a clue???

Cheers in advance

If they aren't sterile then you can use them to filter the solution but I'd suggest pressure cooking the solution after to sterilize.

 

[Hendrix11]

Thanks for the reply, so I could bottle into 10ml bottles, cap and pressure cook in these. Would the pressure cooking not damage the hormone ??

Cheers

 

[Never Been Pinned]

Nope, I think must period pressure cook around 15psi

 

[Notits]

If the vial is capped and sealed, how does the pressure do its job in this situation? We know that in sterilization heat is only part of the solution.

 

[Docd187123]

Thanks for the reply, so I could bottle into 10ml bottles, cap and pressure cook in these. Would the pressure cooking not damage the hormone ??

Cheers

No, it won't damage the hormone. Make sure the vials are clean and sterile though before filling. Also the vials cannot be sitting in the water in the pressure cooker. They must be suspended above the water.

 

[Docd187123]

If the vial is capped and sealed, how does the pressure do its job in this situation? We know that in sterilization heat is only part of the solution.

The pressure, 15PSI, is just used to make water boil at a higher temp. Wet heat penetrates better than dry heat in. It's cases except for oil based solutions which is why I pressure cook my gear longer than the 1hr you generally see mentioned. Heat is actually a very good method of sterilization but the temps required for dry heat sterilization would damage the hormone.

To sterilize my glassware, I put it in the oven at 500deg F for 2hrs or more. This would damage the hormone which is why I only do it for empty glassware.

 

[Benny]

If the vial is capped and sealed, how does the pressure do its job in this situation? We know that in sterilization heat is only part of the solution.

I guess it's like canning. The pressure just allows you to build more heat with out damaging the containers. If you heated sealed vials to that temp with out the pressure they would blow the cap or break the vial due to internal pressure.