Syringe Filters

[HIGHRISK]

maybe make some christmas gifts for a few BROS

What's up BRO!

 

[redbull3906]

Anyone tried one of these? They use a 47mm membrane filters that you can get in various material makeup like you can syringe filters. I couldn't find sterile filters but a little ba should take care of things or they could possibly be dry heat sterilized. The filter simply fits in between the funnel and the flask where the blue clamp is. I haven't got everything in yet but plan on an oil only run of about 250ml to test out.

 

[daylight driller]

Anyone tried one of these? They use a 47mm membrane filters that you can get in various material makeup like you can syringe filters. I couldn't find sterile filters but a little ba should take care of things or they could possibly be dry heat sterilized. The filter simply fits in between the funnel and the flask where the blue clamp is. I haven't got everything in yet but plan on an oil only run of about 250ml to test out.

Yes it is a buchner filter. As long as you are confident in your ability to sterilize it, you should love it. Cuts down on the cost of bottletop filters. Only real problem I have had is once I had to much vacuum and it actually tore the filter so I suggest if using an inline tee with valve to control the vacuum pressure.

 

[daylight driller]

There are some companies that make sterile filter paper. Millapore makes some and if you look in the thread vacuum filtering problem I posted a video that shows you about the filter setup

 

[redbull3906]

I'll look that up. Thanks for the response. I will be using dry heat to sterilize it. I bought an electric pressure cooker but everything is still wet when I open it.

 

[XmadXscientist]

I'll look that up. Thanks for the response. I will be using dry heat to sterilize it. I bought an electric pressure cooker but everything is still wet when I open it.

Make sure you oook up dry heat procedure it's a lot of heat and time. Those electric pressure cookers aren't really true pressure cookers, I would recommend an old school canning one with a psi gauge.

Get these:
http://ivpacks.com/100-autoclave-bags

And the tape to take the guesswork out of it. You can seal stuff in the bag and put it in the pressure cooker keeping it dry.

 

[redbull3906]

I do have those bags but they are paper on one side and the water soaks through. If I used dry heat with the pressure cooker wouldn't I be just as well off in the oven? I would think those bags would burn up in a conventional oven with the heat required to sterilize in that.

 

[master.on]

Just looking for a good, semi fast syringe filter with both a male and female end so I can fill sterile vials.
I looked into bottletop but as a first operation I am looking to stay on a budget while staying sanitary. Only looking to filter about 1L of MCT and Cottonseed carrier. Thanks in advance.

1 The male/female conncectors are called 'luer-lok' sometimes typed as 'luer-lock'
they are standardized so any syringe/needle works

2 top brands are whatman, nalgene, EMD illipore, corning, wvr

3 As you're not filtering any corrosive chemicals or solvents, pretty much any filtering material (i.e. polypropylene, nylon) will do

4 make sure they are 0.22 or 0.2 um and NOT 0.45

5 make sure they are sterile (individually packed)

6 this being said
you'd find plenty of Google results
just stick with large, true laboratory suppliers

 

[-vegeta-]

1 The male/female conncectors are called 'luer-lok' sometimes typed as 'luer-lock'
they are standardized so any syringe/needle works

2 top brands are whatman, nalgene, EMD illipore, corning, wvr

3 As you're not filtering any corrosive chemicals or solvents, pretty much any filtering material (i.e. polypropylene, nylon) will do

4 make sure they are 0.22 or 0.2 um and NOT 0.45

5 make sure they are sterile (individually packed)

6 this being said
you'd find plenty of Google results
just stick with large, true laboratory suppliers

i know they are called luer lock... there is a male and frmale end to a luer lock

im going bottletop filtration so I wont need sterile vials

 

[master.on]

i know they are called luer lock... there is a male and frmale end to a luer lock

im going bottletop filtration so I wont need sterile vials

Be aware that sterile bottles are a must
so either

1 buy sterile bottles, preferably the ones made by the same manufacturer as the bottletop-filter for a perfect fit
2 sterilize bottles in an autoclave before brewing

in an extreme example to show why sterile bottles are badly needed
picture yourself sterile filtering to a dirty toilet lol then drawing from said toilet to pour in sterile vials
obviously the toilet totally ruins sterility

 

[-vegeta-]

Be aware that sterile bottles are a must
so either

1 buy sterile bottles, preferably the ones made by the same manufacturer as the bottletop-filter for a perfect fit
2 sterilize bottles in an autoclave before brewing

in an extreme example to show why sterile bottles are badly needed
picture yourself sterile filtering to a dirty toilet lol then drawing from said toilet to pour in sterile vials
obviously the toilet totally ruins sterility

i have non sterile vials that I am giving an alcohol bath and oven to kill bacteria before filling... then capping and back in the oven.

media bottle is 500mL and will be sterilized the same fashion.

im not buying an autoclave because this isn't going to be a produ tion just for personal use.