Murho
Member
Is there a way to remove the tops from vials so I can sterilize them are reuse them? I don't know how to do it and make sure I don't chip or scratch the glass.
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I dunno i tried all methods of plyers and knives amd i shattered the viles hahaha. I just fill through runner stopper now and wipe the bottle clean with alchohol after each use... i mean its just for me anyways ... if your moving gear shell the money for a kit and cap them yourself dont go crazy more you touch a vile less sterile itll be
I have found using small wire cutters to snip the caps works well.
I resterilize the vials in an autoclave before refilling.
Yeah well i dont have science lab to autoclave lolI have found using small wire cutters to snip the caps works well.
I resterilize the vials in an autoclave before refilling.
If it's just dry heat
Make sure you wrap the vials in Aluminum foil, otherwise they'd become unsterile again the moment you pull them out of the overn.
Dry heat
To sterilize an object with dry heat, you need to place it in an oven at a certain temperature for a certain time.
Authors of textbooks on microbiology do not agree on the temperature and time required, but I find the recommendations of the UK Department of Health quite reasonable: 45 minutes at 170 °C.
Obviously this procedure can be applied only to objects that can stand such conditions.
Glass pipettes are good candidates, as they are generally too long to fit into a pressure cooker.
To sterilize a pipette, first you must substitute the cotton plug on the mouth side. Then wrap the pipette in aluminum foil, to preserve sterility until you need to use it.
Place in the oven and slowly rise temperature to 170°, then keep for 45 minutes.
Turn off the oven and let cool very slowly to avoid breakage due to thermal stress.
Cleaning & Sterilizing
