If you have a decent autoclave and the vials are coming out wet most of the time you have either overloaded your autoclave or you are not using it properly (some models have to have the door cracked during drying).
MESO-Rx
Anabolic Steroids
Sterilizing steam vs dry heat
- Thread starter Jeffg353
- Start date
master.on
New Member
I agree
You mentioned the key word:
decent autoclave
many try to pass glorified pressure cookers as autoclaves.
Jeffg353
New Member
I picked up a pelton n crane autoclave so we'll see how things go once it gets here.
superbane
Member
What's up Doc? Still waiting on that pm thinking of getting an pelton autoclave?
I'm around bro I still pop in at UG AB FM certain things go on that drive my attention away from some of those boards. I've always lurked Meso and came here for info not sure why I have never registered so I did!
Jeffg353
New Member
Here's the autoclave I picked up
Make sure you get the right cleaner for it to
master.on
New Member
@master.on "liquid autoclave" protocol:
1 Wash and rinse vials and stoppers with dishsoap.
2 Leave overnight in 1 N Sodium hydroxide (lye) solution
Sterilization (microbiology) - Wikipedia
3 Sterile filter some methanol with a Nylon syringe filter.
(IDK if sterile filtering methanol is overkill, but do it anyhow, methanol flows quickly thru syringe filters)
4 Rinse vials and stoppers with filtered methanol.
5 allow methanol to evaporate.
6 vials and stoppers are now sterile.
1 Wash and rinse vials and stoppers with dishsoap.
2 Leave overnight in 1 N Sodium hydroxide (lye) solution
Sterilization (microbiology) - Wikipedia
3 Sterile filter some methanol with a Nylon syringe filter.
(IDK if sterile filtering methanol is overkill, but do it anyhow, methanol flows quickly thru syringe filters)
4 Rinse vials and stoppers with filtered methanol.
5 allow methanol to evaporate.
6 vials and stoppers are now sterile.
Pelton and crane makes their own cleaner there are 2 types one goes i to the tak and the other the chamber itself. They also have wipes
Jeffg353
New Member
How often do you recommend cleaning?
Depends on amount of use personally I would do it at least once a month. The cleaner is cheap
Jeffg353
New Member
Any opinion on which cleaner works best... Reservoir tank vs chamber vs wipe?
The manufacturer recommends different ones for different autoclaves. I would go with whatever they recommend
Roco Bama
Member
What exactly are you trying to sterilize ?
Jeffg353
New Member
Anything n everything I can fit into the autoclave...even including a big purple dildo. LOL!
Seriously mostly just my glassware, vials, stoppers...the important shit.
Seriously mostly just my glassware, vials, stoppers...the important shit.
You can get autoclave bags and have stoppers and gloves media bottles already sterilized ready to go
Glass I would do dry heat as it’s much better at destroying endotoxins and pyrogens.
Jeffg353
New Member
That's a good idea.
Jeffg353
New Member
Actually last time I did both autoclave then dry heat. I know it's overkill but I wanted to make sure I overkill any n all chance of contamination/infection.
Sterilization can be achieved through various means, including: heat, chemicals, irradiation, high pressure, and filtration.
According to many credible sources which u can google urself on web, liquid sterilisation is mostly done with autoclave or dry-heat sterilizator
However its not that cut and dry as there are some bacteria which are heat resistent and as well some prions, they remain active even after standard autoclave procedure.
A microfilter with pore size 0.2 µm will usually effectively remove microorganisms.[46] Some staphylococcal species have, however, been shown to be flexible enough to pass through 0.22 µm filters.[47] In the processing of biologics, viruses must be removed or inactivated, requiring the use of nanofilters with a smaller pore size (20-50 nm) are used.
Now in our case i would say that you of course need to use 0.2u to filter all debris and particles and most viruses and bacteria.
And if you want to be super sterile then you would as well autoclave/dry heat sterilize it.
But still, it wont be completly sterile, as 0.2u will not filter all types of microbes, as mentioned.
So there you go, we can make it as sterile as it can get in our environment with our tools.
According to many credible sources which u can google urself on web, liquid sterilisation is mostly done with autoclave or dry-heat sterilizator
However its not that cut and dry as there are some bacteria which are heat resistent and as well some prions, they remain active even after standard autoclave procedure.
A microfilter with pore size 0.2 µm will usually effectively remove microorganisms.[46] Some staphylococcal species have, however, been shown to be flexible enough to pass through 0.22 µm filters.[47] In the processing of biologics, viruses must be removed or inactivated, requiring the use of nanofilters with a smaller pore size (20-50 nm) are used.
Now in our case i would say that you of course need to use 0.2u to filter all debris and particles and most viruses and bacteria.
And if you want to be super sterile then you would as well autoclave/dry heat sterilize it.
But still, it wont be completly sterile, as 0.2u will not filter all types of microbes, as mentioned.
So there you go, we can make it as sterile as it can get in our environment with our tools.
superbane
Member
How much did you get it for?
Do they report these when you buy them?
I've noticed some sellers say they report to local and federal blah blah blah.
Why?
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