Sterilization for stoppers and caps

[jJjburton]

After being able to sterilize the vials. Do you need to sterilize the stoppers and caps?
The stoppers can be sterilized in boiling water I believe. Do the crimper caps have to be sterilized since they are not on the inside of the vial?

It says you can sterilize both the rubber stoppers and vials in boiling water then drying them in oven.

Does anybody know how to sterilize the rubber stoppers correctly?

Vials can just be boiling water and then cover with aluminum in the oven.

 

[Krampus]

Boiling water and dry heat aren't the most ideal sterilization methods but are better than nothing. Aluminum has a nasty oxide, I would avoid using it in sterilization. If you want to be thorough, get a stainless steel pressure cooker that can do over 20lbs. Running it at 20lbs for 30 minutes is the standard in many places I've been. Anything that will touch your solution after filtering should be sterilized by this method. The rubber stoppers can withstand this process well.

Anything outside the vials can be treated with Lysol or similar chemicals such as Wavicide.

 

[jJjburton]

Boiling water and dry heat aren't the most ideal sterilization methods but are better than nothing. Aluminum has a nasty oxide, I would avoid using it in sterilization. If you want to be thorough, get a stainless steel pressure cooker that can do over 20lbs. Running it at 20lbs for 30 minutes is the standard in many places I've been. Anything that will touch your solution after filtering should be sterilized by this method. The rubber stoppers can withstand this process well.

Anything outside the vials can be treated with Lysol or similar chemicals such as Wavicide.

Does the pressure cooker have water? if so then dont you need to dry it?

 

[Krampus]

Yes, it's best to use distilled water. I would get some autoclave bags to keep them in until they're dry and ready for use. Also, if you use a pressure cooker, you probably want use a rack of some sort to elevate the items being sterilized to keep them away from the heating element or burner.

 

[jJjburton]

Yes, it's best to use distilled water. I would get some autoclave bags to keep them in until they're dry and ready for use. Also, if you use a pressure cooker, you probably want use a rack of some sort to elevate the items being sterilized to keep them away from the heating element or burner.

Ugh I was hoping it would be easier then this. So the autoclave bags let water in? or do they keep the vials and stuff dry in the autoclave bags?

 

[jJjburton]

So the metal vials objects that great crimped dont need to be autoclaved becasue they are just on outside. Just the vials and rubber stoppers?

 

[Krampus]

Autoclave bags are generally water permeable paper on at least one side. They will need to dry.

Ideally sterilize the crimping seal too. Just one run shouldn't oxidize the aluminum much at all.

If you're doing small batches for personal use, I would go for per-sterilized sealed vials and syringe filters.

 

[Monstar]

And this is why I use pre sterilized vials. Hard enough with kids to sterilize media bottles no less all of this. That said transfering is a PITA for any kind of volume.

 

[jJjburton]

Autoclave bags are generally water permeable paper on at least one side. They will need to dry.

Ideally sterilize the crimping seal too. Just one run shouldn't oxidize the aluminum much at all.

If you're doing small batches for personal use, I would go for per-sterilized sealed vials and syringe filters.

Ok so then how do you dry the vials and stoppers? This is the part i dont get. Because they are sterilized in water. How can you dry it with it still staying sterilized?

 

[jJjburton]

I wish the rubbing alcohol, boiling water, oven method worked good enough. Thats easy. Maybe once I get this method down it will be easy too. I may stick with pre sterile vials, but would save a TON of money doing it myself.

 

[Krampus]

Just leave it in the bag in a warm and dry place to dry. It may take a while, but it's still sterile as long as it's sealed in the bag.

 

[Big dog]

I use isopropyl alcohol and then bake at 200 degrees for 30 min

 

[jJjburton]

Just leave it in the bag in a warm and dry place to dry. It may take a while, but it's still sterile as long as it's sealed in the bag.

How would you assemble the vials to maintain sterilization. Could you do it while its still in the autoclave bag and dry?

 

[jJjburton]

I use isopropyl alcohol and then bake at 200 degrees for 30 min

For the vials stoppers and sluminum toppers? How do you put them together to maintain sterilzation?

 

[jJjburton]

Boiling water and dry heat aren't the most ideal sterilization methods but are better than nothing. Aluminum has a nasty oxide, I would avoid using it in sterilization. If you want to be thorough, get a stainless steel pressure cooker that can do over 20lbs. Running it at 20lbs for 30 minutes is the standard in many places I've been. Anything that will touch your solution after filtering should be sterilized by this method. The rubber stoppers can withstand this process well.

Anything outside the vials can be treated with Lysol or similar chemicals such as Wavicide.

This is easy enough. Thank you
Just trying to figure out how to assemble, to maintain sterilzation...

Do you need to keep it away from the heated bottom? What would I use som type of stainless steel rack? Like a barbeque rack? to keep everything in this middle.

 

[jJjburton]

https://www.amazon.com/Mealthy-MultiPot-Programmable-Pressure-Stainless/dp/B076QJNK8G/ref=asc_df_B076QJNK8G/?tag=hyprod-20&linkCode=df0&hvadid=241951700600&hvpos=1o9&hvnetw=g&hvrand=16055950139278751591&hvpone=&hvptwo=&hvqmt=&hvdev=c&hvdvcmdl=&hvlocint=&hvlocphy=9004495&hvtargid=pla-399686376919&psc=1

This has a basket and everything. To keep away from heating element. How do you know if it will hit more then 20 lbs?

 

[jJjburton]

I would save the money in just 2 brews from sterilizing...!

 

[balco]

Boiling water and dry heat aren't the most ideal sterilization methods but are better than nothing. Aluminum has a nasty oxide, I would avoid using it in sterilization. If you want to be thorough, get a stainless steel pressure cooker that can do over 20lbs. Running it at 20lbs for 30 minutes is the standard in many places I've been. Anything that will touch your solution after filtering should be sterilized by this method. The rubber stoppers can withstand this process well.

Anything outside the vials can be treated with Lysol or similar chemicals such as Wavicide.

I can't find any 20 psi pressure cookers on Amazon, can you link me to one?

 

[jJjburton]

Boiling water and dry heat aren't the most ideal sterilization methods but are better than nothing. Aluminum has a nasty oxide, I would avoid using it in sterilization. If you want to be thorough, get a stainless steel pressure cooker that can do over 20lbs. Running it at 20lbs for 30 minutes is the standard in many places I've been. Anything that will touch your solution after filtering should be sterilized by this method. The rubber stoppers can withstand this process well.

Anything outside the vials can be treated with Lysol or similar chemicals such as Wavicide.

https://www.walmart.com/ip/110V-18L-Steam-Autoclave-Sterilizer-Dental-Pressure-Sterilization-Dual-Heating/965897920?wmlspartner=wlpa&selectedSellerId=18183&adid=22222222227292170897&wl0=&wl1=g&wl2=c&wl3=354216330692&wl4=pla-760180639322&wl5=9004495&wl6=&wl7=&wl8=&wl9=pla&wl10=125210735&wl11=online&wl12=965897920&veh=sem&gclid=EAIaIQobChMI8bXi8pn15AIVA18NCh2LDgnPEAQYASABEgImYPD_BwE

https://www.walmart.com/ip/110V-18L-Steam-Autoclave-Sterilizer-Dental-Pressure-Sterilization-Dual-Heating/965897920?wmlspartner=wlpa&selectedSellerId=18183&adid=22222222227292170897&wl0=&wl1=g&wl2=c&wl3=354216330692&wl4=pla-760180639322&wl5=9004495&wl6=&wl7=&wl8=&wl9=pla&wl10=125210735&wl11=online&wl12=965897920&veh=sem&gclid=EAIaIQobChMI8bXi8pn15AIVA18NCh2LDgnPEAQYASABEgImYPD_BwE

 

[jJjburton]

I can't find any 20 psi pressure cookers on Amazon, can you link me to one?

Yea me either. What i posted last is the best I found. dental autoclave