Sterilizing finished vials.

[BJJisLIFE]

Hey guys, I'm doing a lot of reading and learning in preparation for my first brew. I came across a video of Asia Pharma's manufacturing process and it shows them autoclaving their open vials before filling, and then autoclaving them again after they've been filled and capped.


I haven't seen any threads about sterilizing finished vials so I figured I'd ask. I was thinking about doing this with my pressure cooker which maxes out at 15psi/250f degrees. Would that kind of heat damage the hormone?

 

[master.on]

I posted the same thing a couple years ago.
Steam doesn't get inside closed vials, so it's only heat involved.
I wonder if heat does anything useful, or if it actually degrades the hormone, or the oil.

 

[Ridethelightningbarefoot]

Bacteria starts to die above 140f. When I brew I always put the finished vials in my oven at 250f for 35 min. Tren is the only compound I know that is really sensitive to heat. I have verified this with bloodwork ( obviously only with test).

 

[Iron Vett]

Bacteria starts to die above 140f. When I brew I always put the finished vials in my oven at 250f for 35 min. Tren is the only compound I know that is really sensitive to heat. I have verified this with bloodwork ( obviously only with test).

Your information, about the temps needed to sterilize, is wrong. I feel compelled to correct this for the readers that follow. The heat required to sterilize is stated in the link below to be 300F for 150 minutes to 340F for 30 minutes. If the oils are brought to this temperature, the oils will have been brought to the point of being smoked and therefore damaged. Heat is to be used to sterilize equipment not oils.

Dry Heat Sterilization: Definition, Process & Validation | Study.com

Edit: I tried to code the link but couldn’t get it to work

 

[BJJisLIFE]

Your information, about the temps needed to sterilize, is wrong. I feel compelled to correct this for the readers that follow. The heat required to sterilize is stated in the link below to be 300F for 150 minutes to 340F for 30 minutes. If the oils are brought to this temperature, the oils will have been brought to the point of being smoked and therefore damaged. Heat is to be used to sterilize equipment not oils.

Dry Heat Sterilization: Definition, Process & Validation | Study.com

Edit: I tried to code the link but couldn’t get it to work

Can you give some insight on the video? If the temperature need to sterilize is above the safe temperatures of the hormone, then what is the purpose of autoclaving the filled vials?

 

[ickyrica]

Heat, among other things, are to sterilize your physical items, prior to filling and capping.

Proper filtration will sterilize your oil, ba, bb, hormone, other solvents, etc.

Put sterilized oil in sterilized vials and seal with sterilized stoppers.

Cap and crimp

Done.

 

[Iron Vett]

Can you give some insight on the video? If the temperature need to sterilize is above the safe temperatures of the hormone, then what is the purpose of autoclaving the filled vials?

Honestly I see no reason that a competent brewer would autoclave the vials after being filled. A competent brewer sterilizes his media bottles, vials and filling equipment. Then filters the oil though a .22um filter into a sterile media bottle then fills the sterile vials and caps and crimps. Easy, peasy. No use to make this stuff hard if it’s not.

 

[ickyrica]

No use to make this stuff hard if it’s not

truth.

 

[Jstone2]

Your information, about the temps needed to sterilize, is wrong. I feel compelled to correct this for the readers that follow. The heat required to sterilize is stated in the link below to be 300F for 150 minutes to 340F for 30 minutes. If the oils are brought to this temperature, the oils will have been brought to the point of being smoked and therefore damaged. Heat is to be used to sterilize equipment not oils.

Dry Heat Sterilization: Definition, Process & Validation | Study.com

Edit: I tried to code the link but couldn’t get it to work

I have posted this as well, and some dipshit tried to argue it was wrong. He asked for studies to prove the numbers i posted, and when i posted the study he said it was wrong. Thanks for posting this for more to see, its nice to see this actually linked. When i posted i was being lazy, and didn't link it.

Sterilize all your equipment with dry heat or an autoclave, and sterilize your oils by filtering them.

 

[Ridethelightningbarefoot]

I have been using this process for quite some time with no sterility issues and I brew my kitchen. So while other methods are also effective I know mine works as well. I mean unless you have a lab quality clean room you risk contamination during the brew process so I think dry heat sterilization is a good option post process in addition to .22 filters. I would never consider brewing without the use of the proper filters. I also buy pre sealed and pre sterilized vials and still take extra steps to make sure I have the best quality I can personally can have for my context
@Iron Vett

 

[BJJisLIFE]

Honestly I see no reason that a competent brewer would autoclave the vials after being filled. A competent brewer sterilizes his media bottles, vials and filling equipment. Then filters the oil though a .22um filter into a sterile media bottle then fills the sterile vials and caps and crimps. Easy, peasy. No use to make this stuff hard if it’s not.

I understand. I'm going through what I'm sure most newbies do before they brew for the first time. The fear of fucking it up. If there are extra steps to ensure I don't fuck up I'll take every last one lol. Thanks for your help, I appreciate it.