From Syringe, through a filter, into PRE CRIMPED PRE STERILIZED VIAL.

[Pineapples4Puss]

So my question to you all.... I have the .22 um filters. But the end is just a big opening to let the liquid fall out, how can I get this filtered liquid into a pre-sealed sterile vial? It doesn't seem to have a place to attach a needle on the filter. so I cant go straight into the sterilized vial as soon as it exits the filter? Posted a pic of the filter I would be using below, Let me know if I'm missing something or if there is another and better way to filter some gear.

Do I backload another syringe to and put an 18 gauge needle on it and do it that way? Or is there something I am missing with the filter tip.

 

[Villain]

So my question to you all.... I have the .22 um filters. But the end is just a big opening to let the liquid fall out, how can I get this filtered liquid into a pre-sealed sterile vial? It doesn't seem to have a place to attach a needle on the filter. so I cant go straight into the sterilized vial as soon as it exits the filter? Posted a pic of the filter I would be using below, Let me know if I'm missing something or if there is another and better way to filter some gear.

Do I backload another syringe to and put an 18 gauge needle on it and do it that way? Or is there something I am missing with the filter tip.

Lol, ask Big Bear Labs because that’s what he was initially doing.

 

[Rival]

From what I was looking at earlier. They make certain .22u filters that have a luer lock slip end for a needle to attach on to. You might have to backload a syringe but I’ll let others chime in.

 

[Jstone2]

You attach a needle to the filter. You also need to add a vent needle to the vial while filliling. No need to back load, just grab a different needle use it to fill the syringe. Then cap the needle and remove it. Attach the filter and needle. Clean the top of the vial, then insert the vent needle. Finally insert the needle attached to the filter and syringe. Now begin filtering into the vial.

 

[limitphobic]

is it a violation to post youtube video links?

 

[BigBaldBeardGuy]

is it a violation to post youtube video links?

Code it.

www.youtube.com

Does that make sense?

 

[limitphobic]

https://www.youtube.com/watch?v=xUsdPAMzw4M

edit: ya learn something new everyday. Thanks Big.
annnnd... heres everything you need to know (I assume)

 

[MisterSuperGod]

Code it.
Does that make sense?

No, it doesn't make sense when we have an embedding function built into the reply box.

 

[Ari O’Speedwagon]

You attach a needle to the filter. You also need to add a vent needle to the vial while filliling. No need to back load, just grab a different needle use it to fill the syringe. Then cap the needle and remove it. Attach the filter and needle. Clean the top of the vial, then insert the vent needle. Finally insert the needle attached to the filter and syringe. Now begin filtering into the vial.

Most of the filters I’ve found don’t have luer locks on both ends for the syringe and the needle. Is there a specific filter you recommend? Don’t you need a luer lock on both ends?

 

[Rival]

Most of the filters I’ve found don’t have luer locks on both ends for the syringe and the needle. Is there a specific filter you recommend? Don’t you need a luer lock on both ends?

the one I picked up with the help of @BigBaldBeardGuy was from gpzmedlab. Item #MPORESF22

• Millipore 33 MM, PVDF Membrane, .22u pore size, sterile syringe filter.

• Luer Lock end for syringe attachment, Luer Slip end for needle attachment.

 

[Ari O’Speedwagon]

the one I picked up with the help of @BigBaldBeardGuy was from gpzmedlab. Item #MPORESF22

• Millipore 33 MM, PVDF Membrane, .22u pore size, sterile syringe filter.

• Luer Lock end for syringe attachment, Luer Slip end for needle attachment.

Thanks to you and BBBG for the help.

 

[BigBaldBeardGuy]

Most of the filters I’ve found don’t have luer locks on both ends for the syringe and the needle. Is there a specific filter you recommend? Don’t you need a luer lock on both ends?

You only need leuer lock on the filter side. There is pressure on that side. The needle side can be slip tip because it’s literally going to be drop by slow painfully slow drop.

 

[Rival]

You only need leuer lock on the filter side. There is pressure on that side. The needle side can be slip tip because it’s literally going to be drop by slow painfully slow drop.

thanks for clarifying, that was kind of tripping me up because I saw some with and without the lock on the needle end. Good to know for next time.

question in regards to using another needle to equalize pressure/vent as you’re filling a sterile vial. Should that needle be towards the top not touching the oil that’s getting pushed in through the filter? Or does it not matter?

 

[Ari O’Speedwagon]

thanks for clarifying, that was kind of tripping me up because I saw some with and without the lock on the needle end. Good to know for next time.

question in regards to using another needle to equalize pressure/vent as you’re filling a sterile vial. Should that needle be towards the top not touching the oil that’s getting pushed in through the filter? Or does it not matter?

Isn’t there a possibility of the oil coming back out through the filter needle if it’s inserted far enough to touch the oil?

 

[Ari O’Speedwagon]

You only need leuer lock on the filter side. There is pressure on that side. The needle side can be slip tip because it’s literally going to be drop by slow painfully slow drop.

I saw some filters on Amazon that highlighted the flow rate with supposedly real graphs comparing their capability with other filters. I was curious if you really wanted the quickest flow or if the flow rate was connected to the overall quality of filtration.
I like to make things complicated.

 

[Rival]

Isn’t there a possibility of the oil coming back out through the filter needle if it’s inserted far enough to touch the oil?

that’s what I’m curious about. Not sure

 

[BigBaldBeardGuy]

I saw some filters on Amazon that highlighted the flow rate with supposedly real graphs comparing their capability with other filters. I was curious if you really wanted the quickest flow or if the flow rate was connected to the overall quality of filtration.
I like to make things complicated.

thanks for clarifying, that was kind of tripping me up because I saw some with and without the lock on the needle end. Good to know for next time.

question in regards to using another needle to equalize pressure/vent as you’re filling a sterile vial. Should that needle be towards the top not touching the oil that’s getting pushed in through the filter? Or does it not matter?

The extra needle is to vent the air out as you fill the vial so it shouldn’t touch the oil. It’s ok if it accidentally does.

The flow rate is just going to determine how long you sit there filtering. It doesn’t have anything to do with the quality of the filtration. The higher rate filters will be a little more expensive but it’ll save some time.

 

[limitphobic]

Do I absolutely need PVDF membrane or would PES membrane filters suffice?

 

[BigBaldBeardGuy]

Do I absolutely need PVDF membrane or would PES membrane filters suffice?

No. No PES.

 

[limitphobic]

No. No PES.

Thanks for the quick reply. Gotta ask though (just curious) why NO to PES?