D
Deleted member 127229
Guest
I'm looking at nylon whatman bottletop filters. They are not sterile
Any practical proven way to sterilize them?
Any practical proven way to sterilize them?
MESO-Rx
Anabolic Steroids
When you say they don't need to be sterilized, that's because they already are or because the 0.2 micron filter gets rid of any issues?
RedSwingline
Member
Are you saying that you re-heat your mixture after filtering and decanting into vials?
Termite
New Member
They will come sterile or not sterile and should be labelled as such.
If they are not sterile, they do need to be sterilized if being used for final product. If you are pre-filtering your carrier oil or something like that then no need to be sterile at that time (I used to pre-filter a lot and store batches of carrier oil, made the final filter easier I found and was worth the time to me, totally unnecessary however). When making gear, the final product does become 'sterile' at the point of sterile filtration.
Plenty of ways to sterilize them. Dry heat sterilization is NOT sterilization via radiation. Radiation is used by manufacturers on a lot of the consumables we might use, then shipped in sealed bags/containers. Radiation is not an option at home. Dry heat sterilization is an option and there are plenty of resources here (check sticky threads) on how to do that. You could also sterilize via autoclave if you have one or pressure cooker if not. Google 'glassware sterilization in pressure cooker' and you can find plenty of info that way. Either way if they are not sterilized, do so in a glass container covered with foil and keep covered until used.
The manufacturer should have specs on whether or not the product is safe for sterilization/types/operating temp/etc. If they are Polypropylene (PP) housing with a nylon filter material they should be fine though.
Some guys will just run a bit of BA through the filter first then call it good. While you might get away with this, it is more like 'sanitation' than sterilization, and is certainly not a best practice.
If they are not sterile, they do need to be sterilized if being used for final product. If you are pre-filtering your carrier oil or something like that then no need to be sterile at that time (I used to pre-filter a lot and store batches of carrier oil, made the final filter easier I found and was worth the time to me, totally unnecessary however). When making gear, the final product does become 'sterile' at the point of sterile filtration.
Plenty of ways to sterilize them. Dry heat sterilization is NOT sterilization via radiation. Radiation is used by manufacturers on a lot of the consumables we might use, then shipped in sealed bags/containers. Radiation is not an option at home. Dry heat sterilization is an option and there are plenty of resources here (check sticky threads) on how to do that. You could also sterilize via autoclave if you have one or pressure cooker if not. Google 'glassware sterilization in pressure cooker' and you can find plenty of info that way. Either way if they are not sterilized, do so in a glass container covered with foil and keep covered until used.
The manufacturer should have specs on whether or not the product is safe for sterilization/types/operating temp/etc. If they are Polypropylene (PP) housing with a nylon filter material they should be fine though.
Some guys will just run a bit of BA through the filter first then call it good. While you might get away with this, it is more like 'sanitation' than sterilization, and is certainly not a best practice.
RedSwingline
Member
Interesting. Do you need to vent the vial while heating? How does the butyl rubber stopper and the flip top dust cover hold up to the oven temps?
Dude this is a odd and weird way, as oil when cooked turns into a different texture and all, wouldnt injects cooked oil
K
klokke23
Guest
Don't do this. The filtration process is the sterilization process. And you want sterilized filters for that. You also don't want to reheat your batch afterwards.
Astartes
Member
This is absolutely and completely wrong.
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Astartes
Member
Buy disposable bottle top filters off Amazon.
They are Gamma irradiated (medical/surgical sterilization) and sealed individually in boxes of 12. Use .45 um then re-filter at .22 um.
Use small aquarium size lab filter pump.
$35 bucks on Amazon and reverse polarity from aquarium. (Don’t buy an actual aquarium pump unless you want well aerated brew. Bigger pumps are also unnecessary for home brew and too much power breaks the filtration media.)
Let run.
If taking too long (Hours for 250-500 ml batch) your issue isn’t pressure it is that you haven’t dissolved the hormone fully. Either heat and dissolve or preferably adjust solvents and carrier oils to dissolve at as low a temp as possible.
Re-filter.
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Flamin
New Member
I've been thinking of going the bottle top filter route. However, how do you go from the media bottle to vials after the filtration? Do you load up syringes or is it just fine to have the bottles exposed to air while doing the transfer with a pipette or something and crimp vials after?
RedSwingline
Member
Generally with the bottle top filter route, you go from a sterilized bottle top filter into a sterilized bottle then transferred from the sterilized bottle to the sterilized open vials via a sterile pipette.
Alot of the new information you are getting is wrong. Look up xkawnx brewing information. He has it all on meso look back at his threads. Dont listen to new members giving you info. Tranferring into open vials without a vent like in a lab, i forgot what there called will always make it not sterile and debri and fall in. If you dont have the vent always filter into a sterile vial with a stopper on top. Some sources will sell presterilzed vials with the stopper on and without the crimp, while if the crimp is on just use a big syringe.
Again, xkawnx brewing bible on meso, he litterally had everyting you need to know.
Edit-I say had because he either changed name and also other reasons.
It also comes down to how careful you want to be about possible dust falling in. Because I promise you if you dont have a flow hood, you will 100000% get dust in an open vial without a stopper on it. Do you know how many times i have tried to put the dam screen protector on my phone, and i see there is no dust on my screen and by the time i place the screen protector on a tiny piece of dust makes it way between, always just one.
