The FDA makes the case for filtering peptides

[Sera]

If you google “sudden allergy to mod grf 1-29 or cjc1295” you will see a slew of reports showing sudden immunogenic responses to that particular drug out of no where even after a long period of time working just fine. I developed an allergy to it after almost a year of using it just fine. The FDA pulled it off market for immunogenicity.

Yeah for sure, I’ve heard about all of these reports. It’s scary to be honest, and one reason why I wouldn’t use these GHRH analogues. What if you had a serious anaphylactic shock?

 

[Sera]

To add some details... This was unfiltered CJC-1295 DAC from Peptide Sciences. Worked fine for me over course of a year (i.e., got expected rise in IGF-1 level), but only worked for first few months for spouse, who then had a single unusually intense response (more intense than typical CJC flushing), after which IGF-1 dropped to below baseline, and w/o further flushing from CJC injections. In addition, after stopping CJC, IGF-1 levels did not recover over several months, leading us to turn to HGH (w/ filtering). This is more or less like what happened with kids who had an immunogenic reaction to exogenous HGH in studies - some recovered, some didn't, some simply needed higher dosing, etc. Anyway, seems prudent to pay attention to things one can do to minimize immunogenic responses to one's native peptides. (As I recall, some of the studies specified exactly the type of antibodies formed, making it clear that the immune system was directly interfering with one's own peptides.)

Thanks for sharing your experience.

I’d be interested to see those studies, I’ll have a search and see if I can find anything.

 

[RandGuy]

I’d be interested to see those studies, I’ll have a search and see if I can find anything.

I have a folder of stuff from Ghoul's posts about this issue, and will put together some links to those. In meantime, here's a paper giving an overview of the immunogenicity issue, although not specific to HGH...
Understanding preclinical and clinical immunogenicity risks in novel biotherapeutics development

 

[RandGuy]

Ghoul's posts on this thread review immunogenicity in detail:

https://thinksteroids.com/community/threads/whats-the-current-state-of-generic-gh.134427828/

 

[HUGE McBIGLARGE]

I don’t have access to pharma bact, but I do have pharma water and eBay’s finest benzyl alcohol. This is what I do:

-crack open a 5ml ampule of water
-draw 2.5ml
-attach filter. Plunge 3 drops of water out the filter
-keep note of how much water remains in the syringe as this determines BA quantity
-remove the filter
-using needle “A”, vent the peptide vial
-attach syringe to needle “A” and plunge the water into the peptide vial, making sure not to foam or bubble
-leave it a few minutes. Check all peptide is dissolved
-draw back out using needle “A”
-remove needle “A”, attach the filter, attach needle “B”
-plunge into a new vial, making sure not to foam or bubble
-remove filter and needle “B”, attach needle “A”
-draw remaining ampule water
-remove needle “A”, reattach filter and needle “B”
-plunge into new vial. Use syringe-less needle “A” to vent pressure during plunging
-disconnect syringe from filter, draw air, reconnect to filter, plunge air into vial
-remove all needles from vial
-using insulin syringe, draw 0.9% BA, then draw some peptide from new vial and replunge into vial. Lightly spin the vial. Wait a few minutes. Can draw peptide with the insulin syringe and inject

I think this accomplishes everything important

 

[Sampei]

I don’t have access to pharma bact, but I do have pharma water and eBay’s finest benzyl alcohol. This is what I do:

-crack open a 5ml ampule of water
-draw 2.5ml
-attach filter. Plunge 3 drops of water out the filter
-keep note of how much water remains in the syringe as this determines BA quantity
-remove the filter
-using needle “A”, vent the peptide vial
-attach syringe to needle “A” and plunge the water into the peptide vial, making sure not to foam or bubble
-leave it a few minutes. Check all peptide is dissolved
-draw back out using needle “A”
-remove needle “A”, attach the filter, attach needle “B”
-plunge into a new vial, making sure not to foam or bubble
-remove filter and needle “B”, attach needle “A”
-draw remaining ampule water
-remove needle “A”, reattach filter and needle “B”
-plunge into new vial. Use syringe-less needle “A” to vent pressure during plunging
-disconnect syringe from filter, draw air, reconnect to filter, plunge air into vial
-remove all needles from vial
-using insulin syringe, draw 0.9% BA, then draw some peptide from new vial and replunge into vial. Lightly spin the vial. Wait a few minutes. Can draw peptide with the insulin syringe and inject

I think this accomplishes everything important

what the fuck I just read AHAHAHAHAH

 

[Ateam2023]

I don’t have access to pharma bact, but I do have pharma water and eBay’s finest benzyl alcohol. This is what I do:

-crack open a 5ml ampule of water
-draw 2.5ml
-attach filter. Plunge 3 drops of water out the filter
-keep note of how much water remains in the syringe as this determines BA quantity
-remove the filter
-using needle “A”, vent the peptide vial
-attach syringe to needle “A” and plunge the water into the peptide vial, making sure not to foam or bubble
-leave it a few minutes. Check all peptide is dissolved
-draw back out using needle “A”
-remove needle “A”, attach the filter, attach needle “B”
-plunge into a new vial, making sure not to foam or bubble
-remove filter and needle “B”, attach needle “A”
-draw remaining ampule water
-remove needle “A”, reattach filter and needle “B”
-plunge into new vial. Use syringe-less needle “A” to vent pressure during plunging
-disconnect syringe from filter, draw air, reconnect to filter, plunge air into vial
-remove all needles from vial
-using insulin syringe, draw 0.9% BA, then draw some peptide from new vial and replunge into vial. Lightly spin the vial. Wait a few minutes. Can draw peptide with the insulin syringe and inject

I think this accomplishes everything important

Damn, that seems like alot, , but at least it works for you,,

 

[AlexDavis43]

I don’t have access to pharma bact, but I do have pharma water and eBay’s finest benzyl alcohol. This is what I do:

-crack open a 5ml ampule of water
-draw 2.5ml
-attach filter. Plunge 3 drops of water out the filter
-keep note of how much water remains in the syringe
-remove the filter
-using needle “A”, vent the peptide vial
-attach syringe to needle “A” and plunge the water into the peptide vial, making sure not to foam or bubble
-leave it a few minutes. Check all peptide is dissolved
-draw back out using needle “A”
-remove needle “A”, attach the filter, attach needle “B”
-plunge into a new vial, making sure not to foam or bubble
-remove filter and needle “B”, attach needle “A”
-draw remaining ampule water
-remove needle “A”, reattach filter and needle “B”
-plunge into new vial. Use syringe-less needle “A” to vent pressure during plunging
-disconnect syringe from filter, draw air, reconnect to filter, plunge air into vial
-remove all needles from vial
-using insulin syringe, draw 0.9% BA, then draw some peptide from new vial and replunge into vial. Lightly spin the vial. Wait a few minutes. Can draw peptide with the insulin syringe and inject

I think this accomplishes everything important

It took more time to read that than it takes to make 100 mL BAC and filter it into three 30 mL vials

Plus I think it's weird that you make BAC directly in the actual peptide vial instead of making larger stock solutions xD

 

[HUGE McBIGLARGE]

what the fuck I just read AHAHAHAHAH

An excellent step-by-step guide to doing things somewhat properly.

I find it funny, too. I laugh so hard when I’m doing it that my neighbours call the police.

Damn, that seems like alot, , but at least it works for you,,

It seems a lot when it’s written like that, but you can do the whole thing in like 2 minutes. Except for the parts where you’ve gotta wait around.

 

[HUGE McBIGLARGE]

It took more time to read that than it takes to make 100 mL BAC and filter it into three 30 mL vials

It’s just the attention to detail. I wrote it for myself so that I can’t fuck anything up.

Plus I think it's weird that you make BAC directly in the actual peptide vial instead of making larger stock solutions xD

It’s more work doing it like that, and it’s easier to contaminate a bact supply.

 

[AlexDavis43]

It’s just the attention to detail. I wrote it for myself so that I can’t fuck anything up.

It’s more work doing it like that, and it’s easier to contaminate a bact supply.

Assuming you don't forget to add the BA, on a scale of 1-10 where do you put the risk of contamination with your BAC recipe vs mine?

Not arguing, just curious how much above 1 anyone would put this.

 

[Sampei]

dude... Nevermind, whatever makes you happy. Just know that you are literally insane and that thing you do has no scientific base to be superior to make a normal vial of bac water and use it.

Your statement imply that even an hospira bac water bottle is less safe then your madness procedure.

oh btw there is so many things that can go wrong and it's so convoluted that... yeah again: whatever xD

It’s just the attention to detail. I wrote it for myself so that I can’t fuck anything up.

It’s more work doing it like that, and it’s easier to contaminate a bact supply.

 

[HUGE McBIGLARGE]

Assuming you don't forget to add the BA

I make sure I can’t forget it

on a scale of 1-10 where do you put the risk of contamination with your BAC recipe vs mine?

Not much different. But it seems very pointless to crack open a load of perfectly sterile pharma grade ampules to move them into multi-use vials. Instead, I can use 1 ampule per peptide reconstitution.

that thing you do has no scientific base to be superior to make a normal vial of bac water and use it.

It avoids unnecessarily opening sterile ampules. So it is superior to a degree greater than zero.

Your statement imply that even an hospira bac water bottle is less safe then your madness procedure.

What’s so mad about it?

oh btw there is so many things that can go wrong

Tell me

 

[AlexDavis43]

Not much different. But it seems very pointless to crack open a load of perfectly sterile pharma grade ampules to move them into multi-use vials. Instead, I can use 1 ampule per peptide reconstitution.

I use regular sterile water from Amazon and let BA do it's thing

Not sorry to any of the bubble boys reading this

 

[HUGE McBIGLARGE]

I use regular sterile water from Amazon and let BA do it's thing

Not sorry to any of the bubble boys reading this

The water and BA pharma grade?

 

[HUGE McBIGLARGE]

Don’t take it personally that I apparently do things differently to you lot.

I understand that people are naturally inclined to defend their practices and attack the practices of others. But if you wanna call me a fucking madman for doing things my way, please back it up with 1 or more reasons why.

 

[rockyraccoon]

Don’t take it personally that I apparently do things differently to you lot.

I understand that people are naturally inclined to defend their practices and attack the practices of others. But if you wanna call me a fucking madman for doing things my way, please back it up with 1 or more reasons why.

1) How accurate are your syringes that you can measure 3 drops? lol
2) How are you ensuring that the needles n shit are still clean after all the switcheroos?
3) At what point are you actually filtering the BA?

 

[HUGE McBIGLARGE]

1) How accurate are your syringes that you can measure 3 drops? lol

I just eyeball it. It’s to flush out the filter first, as I’m not using pharma grade filters.

2) How are you ensuring that the needles n shit are still clean after all the switcheroos?

I put the needles back into their guards between switching.

3) At what point are you actually filtering the BA?

I figure ~0.045ml of BA is likely to have such a low amount of absolute impurities to not warrant the risk of the filter retaining some of the BA.


If I was making a large batch of bacteriostatic water, I’d filter it.

 

[rockyraccoon]

I just eyeball it. It’s to flush out the filter first, as I’m not using pharma grade filters.

I put the needles back into their guards between switching.

I figure ~0.045ml of BA is likely to have such a low amount of absolute impurities to not warrant the risk of the filter retaining some of the BA.


If I was making a large batch of bacteriostatic water, I’d filter it.

Fair enough. You do you, bro. I guess it's just one of those zen moments for you lol

 

[AlexDavis43]

The water and BA pharma grade?

USP, nothing fancy

Tbh I don't really think about it much... it's just BAC water (hopefully not famous last words)