Injectable Glutathione Recipe

[JustJoe]

What displacement are u using? ~.7?
Why not just do a 700mg/ml and pin less liquid.
At 800mg/ml i could not push through a .22 filter

Can that EDTA be filtered out?
Isn't that a chelation agent?

I used all my GSH raws. I was aiming for 600mg then diluted it too much using too weak NaOH during ph bal.

I kind of want the EDTA to completely dissolve.

I’m (attempting) using L-Histadine as a buffer instead of PBS, PBS=pip for me; EDTA as a chelator (binds to any trace heavy metals and makes them water soluble and tissue inactive, washing out through kidneys), and Sodium Metabisufite (SMB) and BA 0.9 as a preservatives.

l-Histadine is a completely pain free buffer, used on some vaccines.

When I get more raws, make it work, and check the PIP, I’ll post the formulation.

Here’s a cool quick read:

https://www.pharmaexcipients.com/wp-content/uploads/attachments/257115883-Excipient-for-parenteral.pdf

This one talks more about L Histadine as buffer:

Histidine in Health and Disease: Metabolism, Physiological Importance, and Use as a Supplement - PMC

L-histidine (HIS) is an essential amino acid with unique roles in proton buffering, metal ion chelation, scavenging of reactive oxygen and nitrogen species, erythropoiesis, and the histaminergic system. Several HIS-rich proteins (e.g., ...

pmc.ncbi.nlm.nih.gov

 

[Photon]

I used all my GSH raws. I was aiming for 600mg then diluted it too much using too weak NaOH during ph bal.

I kind of want the EDTA to completely dissolve.

I’m (attempting) using L-Histadine as a buffer instead of PBS, PBS=pip for me; EDTA as a chelator (binds to any trace heavy metals and makes them water soluble and tissue inactive, washing out through kidneys), and Sodium Metabisufite (SMB) and BA 0.9 as a preservatives.

l-Histadine is a completely pain free buffer, used on some vaccines.

When I get more raws, make it work, and check the PIP, I’ll post the formulation.

Here’s a cool quick read:

https://www.pharmaexcipients.com/wp-content/uploads/attachments/257115883-Excipient-for-parenteral.pdf

This one talks more about L Histadine as buffer:

Histidine in Health and Disease: Metabolism, Physiological Importance, and Use as a Supplement - PMC

L-histidine (HIS) is an essential amino acid with unique roles in proton buffering, metal ion chelation, scavenging of reactive oxygen and nitrogen species, erythropoiesis, and the histaminergic system. Several HIS-rich proteins (e.g., ...

pmc.ncbi.nlm.nih.gov

So you're hoping to get a chelating effect as well by including EDTA?
For me I used 50% naOH with naCL. I later realised the 50% naOh is so strong lol, you really only need a small amount of it. I'll probably just do bac with 50% naOh next time.

 

[JustJoe]

So you're hoping to get a chelating effect as well by including EDTA?
For me I used 50% naOH with naCL. I later realised the 50% naOh is so strong lol, you really only need a small amount of it. I'll probably just do bac with 50% naOh next time.

Yeah 50% NaOH is ~19M, which is really strong, it would be like syrup, drain cleaner. and it would probably even if u you just dropwise, each drop will increase the temp as it reacts with the solution around it. That’s why pellets ruin it, each tiny pellet raises the temp to like maybe 70°c or 80° in a little hot bubble with the surrounding solution as it dissolves, which I think oxidizes the GSH. Next experiment I’m going to try using 10M NaOH, which is 12g dry NaOh mixed into 30mls of sterile WFI.

 

[Deskoze38192]

You can just do sodium chloride (saline) + glut for 200mg/ml.
Probably wont even need sodium hydroxide.
Should be relatively easy to brew.

NAC has poor oral bioavailability (~10%), Only a small amount of NAC converts to glut. it is also more acidic than glut and needs to stay at a lower pH to not degrade. Lower pH injections sting more. Both are however acidic and you should have no issues mixing both.

NAC can be brewed at much higher concentrations, similar to L-Carnitine.

Excellent info thank you!
Appreciate it!

 

[Deskoze38192]

I do not think adding NAC is necessary. NAC is converted to cysteine in the body, cysteine is the rate-limiting amino acid in glutathione synthesis. So adding a ton of NAC does not make sense to me seeing as how you are already injecting glutathione. NAC has other functions so I still supplement it at 500 mg a day orally. You can overdo antioxidants.

Thank you I appreciate this info
Yes I already take NAC at 500 mg per day
Think I’ll just start glut brew at 200 mg/mL and then work my way up to higher concentrations

What amounts are yall brewing at a time?
Smaller amounts because of degradation?

 

[JustJoe]

GSH on its own without ph balancing is like 2.8ph, way too acidic to inject, even with saline, right?

Korean lypholized Glutaone says it’s ph balanced with bicarb but IDK how.

 

[Photon]

GSH on its own without ph balancing is like 2.8ph, way too acidic to inject, even with saline, right?

Korean lypholized Glutaone says it’s ph balanced with bicarb but IDK how.

I'm not exactly sure how they do it.
They brew it, then freeze dry it with the bicarb etc?
No clue but it's alot less thick at the same mg/ml than a homebrew.

You can do 800mg/ml with Korea Pharma.
A homebrew? lol you cant even filter it.

Crystal clear at ph 6.4.
That 1 vial has like 7 vials reconstituted inside, i then add GHKCU to it.

Post in thread 'Glutathione help pls'

Jul 16, 2025

That is crazy. DM or post the pharmacy if you don't mind. I have to figure out that brew. I can't pin 200mg without massive pip for a day or 2. Sometimes longer.

Do you have pH tester or strips? I would be really curious what its at.

The PH was not adjusted etc, all i did was add GHKCU and BAC.

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[Photon]

Thank you I appreciate this info
Yes I already take NAC at 500 mg per day
Think I’ll just start glut brew at 200 mg/mL and then work my way up to higher concentrations

What amounts are yall brewing at a time?
Smaller amounts because of degradation?

I do 150ml which lasts me 5 months.
I'm running an experiment now with 1ml daily and pulling ALT labwork every 3 weeks or so. If my ALT remains low every lab pull, Im just going to assume the glut has not deteriorated significantly, if any at all.

There's a patent or study (don't remember) somewhere which shows pH 6.4 @ fridge temperatures showing no degradation over 6+ months.

 

[Deskoze38192]

I do 150ml which lasts me 5 months.
I'm running an experiment now with 1ml daily and pulling ALT labwork every 3 weeks or so. If my ALT remains low every lab pull, Im just going to assume the glut has not deteriorated significantly, if any at all.

There's a patent or study (don't remember) somewhere which shows pH 6.4 @ fridge temperatures showing no degradation over 6+ months.

Okay great! 6.4 ph and fridge iit is!
Probably do the same 150 mL
Thanks!

I had been using bulk supplements for my B-12, L-Carnitine brews. Guess they are not great quality
What brands are some of yall using for vitamin, amino raw powder

 

[Sampei]

I may have salvaged mine. I added 10mg more of EDTA which binds to heavy metals a chelates them, . My solution is almost clear now.
If the EDTA addition pulled most of the pink out, then my color shift was from trace metal contamination rather than bulk GSH oxidation. Probably in the NaOH pellets.

Not sure if I feel comfortable trying this, but I may. It worked out 294mg/ml

Also, not all the EDTA is dissolved yet, which usually takes raising the temp <sweating and smiling nervously emoji>

Why the cover over the beaker? XD

 

[Meowmeow83]

So instead of NaOh I use sodium bicarbonate- aka regular ass baking soda. Works great

Here are my ratios

Glutathione



200mg/ml

2g per 10ml

4g per 20ml

6g per 30 ml

12g per 60 ml

1.6grms of bicarbonate per 20 ml

 

[Meowmeow83]

Has anyone seen their glut change color..when they add BA?
Kinda annoyed that mine turned red after i added 1% BA..
BA is acidic too..doesn't even make sense..
~650mg/ml..

View attachment 338508
View attachment 338509

This happens- I’m using bicarb - it bubbles - then turns pink then eventually goes back to clear.

 

[Meowmeow83]

It’s a tricky bitch. Pink means you oxidized some of the GSH, and honestly it’s ruined.

I crying over a beaker right now that was supposed to be 30ml: obviously it grew. Was adding 1M NaOH and the ph was raising .1 with every 3ml, I got frustrated and started added pellets.

I added the NaOH too fast, it got too hot, oxidized and turned pink at 6.5 pH. Tried adding SMB and EDTA to salvage, it went from red to pink but no dice.

It’s Trash

Lesson: add >10M NaOH in solution SLOWLY
View attachment 340734

Oh interesting- it turns pink for me and it went clear afterwards . Maybe I’ll try with NaOh instead.

 

[JustJoe]

Yessssss I did it. Sting free.
L-histadine buffered, preserved shelf-stable GSH 300mg/ml at 6.5pH

Next batch going to go for 600mg

 

[JustJoe]

Why the cover over the beaker? XD

I left it stirring for about 2 hours and I don’t want too much oxygen exposure during the vortex. And it’s not under a laminar hood so I don’t want to sneeze and endotoxin micro droplets get it it. Also the beaker was way more full than I planned. Usually in a lab you would use watch glass to put on top but I don’t have any

 

[JustJoe]

I'm not exactly sure how they do it.
They brew it, then freeze dry it with the bicarb etc?
No clue but it's alot less thick at the same mg/ml than a homebrew.

You can do 800mg/ml with Korea Pharma.
A homebrew? lol you cant even filter it.

Crystal clear at ph 6.4.
That 1 vial has like 7 vials reconstituted inside, i then add GHKCU to it.

Post in thread 'Glutathione help pls'

Jul 16, 2025

That is crazy. DM or post the pharmacy if you don't mind. I have to figure out that brew. I can't pin 200mg without massive pip for a day or 2. Sometimes longer.

Do you have pH tester or strips? I would be really curious what its at.

The PH was not adjusted etc, all i did was add GHKCU and BAC.

• Photon

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I bet they add GSH base to a concentrated bicarb solution very very slowly. They probably have a known concentration. For lypholization they just remove water by placing in vacuum under heat

 

[Pez'sAnabolicJunta]

I really struggled to get it right and I wanna save people looking for the recipe some time so they don't have to go through all posts.

This is the recipe for 30 ml at 200 mg/ml:

1) Weigh out 6 grams of Reduced Glutathione powder and pour it in your mixing beaker.
2) Add around 20 ml of Bacteriostatic water and start mixing (I prefer magnetic stirrer).
3) After a couple of minutes a milky cloudy solution will form. To get it to completely dissolve you will need to increase te pH of the solution.
4) Add NaOH pellets or NaOH solution to your mix. Add it very slowly, ml by ml or a couple of mg by couple of mg. If you get the pH above 6.5 it will oxidize instantly turning red and ruining your product. Having a pH meter is a life saver. The solution will start clearing up before 4.0 but I like to keep my brew between 4.5 and 4.9.
5) Once you've achieved your desierd pH, the solution should be crystal clear.
6) Add bacteriostatic water up to 30 ml and mix for another 5-6 minutes.
6) You can now filter your solution into a vials through 0.22 micron filter (I prefer Nylon66).
7) Once you've done you should store your Glutathione. If you are going to use it in the next 1-2 months you can store it in the fridge under 5 degrees C. If you are not going to use it soon then you should put it in the freezer and unfreeze it when you are ready to use it.

I tried Acetyl-L-Caritine w/o understanding that I needed NaOh and citric acid along with a pH meter. That was 15yrs ago. I am going to try lots of others for the hell of it!

 

[JustJoe]

here is what I found out:

Why the cover over the beaker? XD

Here's a more articulated answer from my ChemGPT:

Covering the beaker during preparation is important because glutathione oxidizes rapidly when exposed to oxygen and light; keeping it covered (and ideally under inert gas) minimizes air contact, reduces oxidation, and prevents contaminants from falling in.

Korean pharma companies that make glutathione injectables with only sodium bicarbonate listed as the inactive ingredient typically start with very pure, pharmaceutical-grade GSH and dissolve it under an inert gas like nitrogen or argon to prevent oxidation, using stainless steel surfaces that are passivated, ususally PTFE-lined, to avoid metal leaching.


The raw solution is acidic, so they add enough sterile sodium bicarbonate to bring the pH into the physiological range (around 7.0–8.0) while allowing the CO₂ produced to off-gas. The product is then lyophilized (freeze-dried) under vacuum and sealed in nitrogen-filled vials, which makes it stable without other antioxidants or chelators.

Here's its judgy answer to why using a laminar flow hood is paramount which compounding injectables, even with 0.22um filtration as final step. Im in the process of modifying my old 3d printing enclosure into a laminar hood. WIP

Producing injectables without a laminar flow hood is still very dangerous even if you use 0.22 micron filtration. The filter can remove most bacteria, but it will not remove all endotoxins, exotoxins, viruses, or pyrogens, and these can still cause severe reactions or death if injected. Without a hood, airborne dust, skin cells, and microorganisms can contaminate your solution during preparation, and some contaminants can pass through the filter or be introduced after filtration during handling and filling. A laminar flow hood provides the sterile, HEPA-filtered environment needed to minimize contamination risk during every step of compounding, which is essential for safe injectable production.

That being said, most of us in this section of the forum have pretty high risk tolerance, and home brew has been going on for a long time, and I dont personally know anyone that has used home-brew Test for example and gotten sick, although I think pathogens are much less likely to grow in oil bases.

 

[MapleAUS]

Yes this is exactly what I did and it works perfectly, It's 10 g of powder total.. you can mix up the glutathione and bac water first and have the stir bar turned on, It just looks like a snow globe because it doesn't dissolve worth of shit, then as soon as you add the six grams of carnitine, It will turn crystal clear, then you can top it off with bac to get exactly 20 ml, I forget how many mils of bac I started with.. 8 or 10 maybe.. you have to factor in the displacement of the powder so you don't just start with 20 mils of bac water, you start with less, make it hold into the solution, then top it off

Did anyone end up pinning the Carnitine + Glut brew IM? Or only an IV trial. IIRC ph was 5?

Would love to brew this if i get confirmation, save an extra pin a day

 

[dinfar1337]

Did anyone end up pinning the Carnitine + Glut brew IM? Or only an IV trial. IIRC ph was 5?

im guessing most people do IM here, but i live with a family member who is a nurse and can do IV on me so would be nice to hear if some has done IV if its worth it for me