Filtering hgh

Ok. I think I’ve figured out why I’m having no success filtering hgh without aggregate forming.

(To clarify (pun not intended): filtering does improve the appearance but there are always notable, possibly immunogenic, visible to the naked eye, aggregate flocs)

Basically even with a very low protein binding membrane, the 0.22 is fouled almost instantly due to the high quantities of large ‘aggregate’. (Visible when the freshly reconstituted solution is inspected with a light, every brand of ugl hgh I’ve tried)

This forms a hydrophobic cake that interacts with the monomers being filtered (and increasing shear) , causing aggregation in the filtered solution.

Filtration failure.

The solution, likely is to use a non sterile (cheaper) 5 micron pes or pvdf or similarly large pre-filter to draw up the hgh, and then for the final sterilising pass, use a 0.2.
 
Ok. I think I’ve figured out why I’m having no success filtering hgh without aggregate forming.

(To clarify (pun not intended): filtering does improve the appearance but there are always notable, possibly immunogenic, visible to the naked eye, aggregate flocs)

Basically even with a very low protein binding membrane, the 0.22 is fouled almost instantly due to the high quantities of large ‘aggregate’. (Visible when the freshly reconstituted solution is inspected with a light, every brand of ugl hgh I’ve tried)

This forms a hydrophobic cake that interacts with the monomers being filtered (and increasing shear) , causing aggregation in the filtered solution.

Filtration failure.

The solution, likely is to use a non sterile (cheaper) 5 micron pes or pvdf or similarly large pre-filter to draw up the hgh, and then for the final sterilising pass, use a 0.2.
This is why I don't want to start down the "filter my hgh" rabbit hole, i can Respect your efforts , but it seems like you might be wasting alot of hgh in the process? Idk
 
This is why I don't want to start down the "filter my hgh" rabbit hole, i can Respect your efforts , but it seems like you might be wasting alot of hgh in the process? Idk
To some extent it’s just a challenge for myself to see if I can do it, and then it might be valuable information to put out there for others reading in the future.

If people have been using unfiltered hgh for years without notice a drop in effectiveness then who knows maybe it’s fine, everyone has there own comfort level.

I did see one guy on here who was doing 60iu a day which made me think there might be some truth to the whole immunity thing lol.
 
To some extent it’s just a challenge for myself to see if I can do it, and then it might be valuable information to put out there for others reading in the future.
I just switched to 15iu vials from LSPL and its the only clear vials I've had out of SSA, SRY, and Lobster. Concentration is the problem I'm 99% sure. Anyone buying 24iu+ is injecting aggregates without filtering, regardless of what the forum dweebs say.
 
Ok. I think I’ve figured out why I’m having no success filtering hgh without aggregate forming.

(To clarify (pun not intended): filtering does improve the appearance but there are always notable, possibly immunogenic, visible to the naked eye, aggregate flocs)

Basically even with a very low protein binding membrane, the 0.22 is fouled almost instantly due to the high quantities of large ‘aggregate’. (Visible when the freshly reconstituted solution is inspected with a light, every brand of ugl hgh I’ve tried)

This forms a hydrophobic cake that interacts with the monomers being filtered (and increasing shear) , causing aggregation in the filtered solution.

Filtration failure.

The solution, likely is to use a non sterile (cheaper) 5 micron pes or pvdf or similarly large pre-filter to draw up the hgh, and then for the final sterilising pass, use a 0.2.
What concentration are you reconstituting to?
 
I just switched to 15iu vials from LSPL and its the only clear vials I've had out of SSA, SRY, and Lobster. Concentration is the problem I'm 99% sure. Anyone buying 24iu+ is injecting aggregates without filtering, regardless of what the forum dweebs say.
How much water do you use with that?
 
To some extent it’s just a challenge for myself to see if I can do it, and then it might be valuable information to put out there for others reading in the future.

If people have been using unfiltered hgh for years without notice a drop in effectiveness then who knows maybe it’s fine, everyone has there own comfort level.

I did see one guy on here who was doing 60iu a day which made me think there might be some truth to the whole immunity thing lol.

Your experience is completely anomalous.

There’s no shortage of people here filtering without creating visible aggregation. I’ve filtered hundreds of vials of rHGH and never had that issue.

I brought up the topic of sheer stress here, because I found references to it when I was researching whether there was any possibility of harm from filtering. It reflected the fact I was keeping an open mind to both positive and negative aspects of the entire concept. The end result was that it was an issue engineers setting up industrial production of rHGH encountered when using very high pressures and small aperture metal tubes, nothing close to the pressure of hand pressed syringes.

It’s been done under carefully controlled laboratory conditions, using off the shelf consumer syringes and syringe filters, looking for damage or lost rHGH using testing equipment that goes well beyond what Jano’s lab has, by professional scientists including a recognized industry leading innovator who formulates peptide and protein drug delivery systems for pharma.

It’s been demonstrated to remove massive amounts of aggregates and other particulate matter without losing rHGH or damaging its structure making it more prone to aggregation.

I don’t know what’s going on in your situation, but it’s 100% something in your specific set up or technique, not evidence that “filtering harms rHGH”.

I can show proof of any of this. As I said, I went down every avenue, positive and negative, before coming to a conclusion, and continue to update my knowledge on the topic.

Filtering does not inherently harm rHGH, sterilizes what is frequently unsterile, removes inorganic particulates, and reduces the widely recognized risk of aggregates.

We’re talking about harms in which the risk is one thats cumulative over the long term, causing damage that would be extremely unlikely to have a clear line back to contaminants from unregulated injectable drugs made in a country renowned for having no concern for consumer safety.

That lack of concern is in above board industries like baby formula that have some oversight.

The dishonest, ongoing anti-harm reduction campaign by a (very) few would have you believe Chinese criminals, knowing they can never be held accountable are more concerned about your well being than legal companies in a culture that’s poisoned infants, recycled oil scavenged from sewers for food prep, and worse to boost profits a small amount. That if harms are not immediately apparent, or won’t appear for years, they shouldn’t be taken seriously. That the fact pharma regulators and companies invest enormous amounts of time and money to minimize aggregates isn’t evidence of how serious potential harms are, it’s just a conspiracy of bubble boys chasing phantoms because the only acceptable proof would be clinical trials intentionally injecting garbage into subjects for years to see what harm it causes them.

If anyone has any doubt as to what I’m saying here about the recognized risks that Pharma and regulators work hard to minimize, which filtering goes some small way to replicate and bring UGL a little closer to the safety standards of Pharma, feel free to ask. I have the receipts.

If you don’t care to reduce risk, I’m not judging you. Anyone with zero risk tolerance wouldn’t use UGL anything..

But for those that do care, and are interested in their long term well being, don’t be lulled into complacency by those who discount what’s of great concern to the professionals who set the quality standards and produce the pharma versions we’d all use if cost and access were not an issue.

Worse, beware the dishonest claims that filtering increases harm, because like arguments that that tell people what they want to hear, it’s very seductive and easy to swallow. It justifies the low effort path by presenting it as the real way to minimize harm. One that says “believe me, and not only will it require zero effort going forward, you can feel good about the way you’ve done things up to now.”.
 
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@Ghoul


I entirely agree with you in terms of not using unfiltered peptides.

I was just wondering aloud whether there was any evidence of reduced efficacy (due to neutralizing antibodies) or long-term baseline IGF-1 reduction in those who have used unfiltered HGH, I suppose.

It doesn’t seem we have an epidemic of users having to take huge doses. Although I definitely have seen that with GLP-1s.
 
Your experience is completely anomalous.

There’s no shortage of people here filtering without creating visible aggregation. I’ve filtered hundreds of vials of rHGH and never had that issue.

I brought up the topic of sheer stress here, because I found references to it when I was researching whether there was any possibility of harm from filtering. It reflected the fact I was keeping an open mind to both positive and negative aspects of the entire concept. The end result was that it was an issue engineers setting up industrial production of rHGH encountered when using very high pressures and small aperture metal tubes, nothing close to the pressure of hand pressed syringes.

It’s been done under carefully controlled laboratory conditions, using off the shelf consumer syringes and syringe filters, looking for damage or lost rHGH using testing equipment that goes well beyond what Jano’s lab has, by professional scientists including a recognized industry leading innovator who formulates peptide and protein drug delivery systems for pharma.

It’s been demonstrated to remove massive amounts of aggregates and other particulate matter without losing rHGH or damaging its structure making it more prone to aggregation.

I don’t know what’s going on in your situation, but it’s 100% something in your specific set up or technique, not evidence that “filtering harms rHGH”.

I can show proof of any of this. As I said, I went down every avenue, positive and negative, before coming to a conclusion, and continue to update my knowledge on the topic.

Filtering does not inherently harm rHGH, sterilizes what is frequently unsterile, removes inorganic particulates, and reduces the widely recognized risk of aggregates.

We’re talking about harms in which the risk is one thats cumulative over the long term, causing damage that would be extremely unlikely to have a clear line back to contaminants from unregulated injectable drugs made in a country renowned for having no concern for consumer safety.

That lack of concern is in above board industries like baby formula that have some oversight.

The dishonest, ongoing anti-harm reduction campaign by a (very) few would have you believe Chinese criminals, knowing they can never be held accountable are more concerned about your well being than legal companies in a culture that’s poisoned infants, recycled oil scavenged from sewers for food prep, and worse to boost profits a small amount. That if harms are not immediately apparent, or won’t appear for years, they shouldn’t be taken seriously. That the fact pharma regulators and companies invest enormous amounts of time and money to minimize aggregates isn’t evidence of how serious potential harms are, it’s just a conspiracy of bubble boys chasing phantoms because the only acceptable proof would be clinical trials intentionally injecting garbage into subjects for years to see what harm it causes them.

If anyone has any doubt as to what I’m saying here about the recognized risks that Pharma and regulators work hard to minimize, which filtering goes some small way to replicate and bring UGL a little closer to the safety standards of Pharma, feel free to ask. I have the receipts.

If you don’t care to reduce risk, I’m not judging you. Anyone with zero risk tolerance wouldn’t use UGL anything..

But for those that do care, and are interested in their long term well being, don’t be lulled into complacency by those who discount what’s of great concern to the professionals who set the quality standards and produce the pharma versions we’d all use if cost and access were not an issue.

Worse, beware the dishonest claims that filtering increases harm, because like arguments that that tell people what they want to hear, it’s very seductive and easy to swallow. It justifies the low effort path by presenting it as the real way to minimize harm. One that says “believe me, and not only will it require zero effort going forward, you can feel good about the way you’ve done things up to now.”.
Great overview.

Once you get in the habit of filtering, it’s just part of the deal, can get it done quickly and do a bunch of peps at the same time for longer term usage.

And with all the saving costs over pharma, a cheap filter seems a no brainer
 
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Sorry if it's a dumb question, but are filters single-use? What I mean by that is, draw - put on a filter and needle - inject into the sterile vial, and after that, dispose of and open a new filter?
 
Sorry if it's a dumb question, but are filters single-use? What I mean by that is, draw - put on a filter and needle - inject into the sterile vial, and after that, dispose of and open a new filter?
No stupid questions here, think everyone can learn something! I filter a bunch at one time, and from what I’ve seen is that you can use it a few times before it either breaks or is unusable. As long as I’m diligent with using new sterile needles and wiping down surfaces with a wipe then I think it’s good to reuse
 
What type of filters do you guys use?
I use 0.22 um Pes filters, again from what I’ve researched, Pes is the best material for not leeching out as much peptides as possible and the 0.22 is the smallest filter length so again doesn’t stop a lot of the peptide, I stupidly bought a 50 pack the first time, but realised they need to be sterile individually wrapped ones…
 
No stupid questions here, think everyone can learn something! I filter a bunch at one time, and from what I’ve seen is that you can use it a few times before it either breaks or is unusable. As long as I’m diligent with using new sterile needles and wiping down surfaces with a wipe then I think it’s good to reuse
Once you open the packaging and put hgh in that filter, it's no longer sterile. It's not something you pull out of a dirty bag and reuse a few days later.
 
Yeah that’s why I only use the filter when I batch filter a bunch. Once the needle with filter is in the sterile vial, I leave it in there and I detach the syringes to fill them up again, reattach to the filter and push through more.
 
I use 0.22 um Pes filters, again from what I’ve researched, Pes is the best material for not leeching out as much peptides as possible and the 0.22 is the smallest filter length so again doesn’t stop a lot of the peptide, I stupidly bought a 50 pack the first time, but realised they need to be sterile individually wrapped ones…
From what I understand, pes filters are not compatible with BA. I know .9% is super tiny amount of BA when dealing with a few ml. But does that worry you at all?

Have you thought of using hydrophilic pvdf?

They have a slightly high protein binding, but are compatible with BA .
 
From what I understand, pes filters are not compatible with BA. I know .9% is super tiny amount of BA when dealing with a few ml. But does that worry you at all?

Have you thought of using hydrophilic pvdf?

They have a slightly high protein binding, but are compatible with BA .
Doesn’t look like it’s a problem because Pes is low protein binding

Extracted from https://www.agilent.com/cs/library/...fA8U5BYtQbz-bQ7ojb3Q7_GEM1c61oYsipkGtZvCyLX5D



Agilent Captiva Premium PES syringe filters were evaluated and compared with other PES and PVDF syringe filters for filtration recovery, targeted to protein analysis and chemical cleanliness. For protein analysis, Captiva Premium PES syringe filters provided excellent and consistent filtration recovery for different proteins at low to high concentrations.


Compared to other PES and PVDF syringe filters, Captiva Premium PES syringe filters clearly teatured extremely low protein binding, thus significantly preventing protein analyte loss during filtration. In addition, they were also cleaner than other syringe filters, and therefore, did not cause chemical contamination during filtration. Captiva Premium PES syringe filters are the best option for the filtration of biological samples.”
 
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