Filtering hgh

[V.primal]

Doesn’t look like it’s a problem because Pes is low protein binding

Extracted from https://www.agilent.com/cs/library/...fA8U5BYtQbz-bQ7ojb3Q7_GEM1c61oYsipkGtZvCyLX5D


“
Agilent Captiva Premium PES syringe filters were evaluated and compared with other PES and PVDF syringe filters for filtration recovery, targeted to protein analysis and chemical cleanliness. For protein analysis, Captiva Premium PES syringe filters provided excellent and consistent filtration recovery for different proteins at low to high concentrations.


Compared to other PES and PVDF syringe filters, Captiva Premium PES syringe filters clearly teatured extremely low protein binding, thus significantly preventing protein analyte loss during filtration. In addition, they were also cleaner than other syringe filters, and therefore, did not cause chemical contamination during filtration. Captiva Premium PES syringe filters are the best option for the filtration of biological samples.”

Yes, I agree that pes are the lowest protein binding filter.
My concern is about it's compatibility with benzyl alcohol that's in bacteriostatic water

 

[Spaceman Spiff]

Yes, I agree that pes are the lowest protein binding filter.
My concern is about it's compatibility with benzyl alcohol that's in bacteriostatic water

Interesting find. I have only filtered peptides when did 20 vials at once and I knew the needle will dull.

Looks like it's not compatible

Tisch Scientific | Material Compatibility

www.tischscientific.com

 

[V.primal]

Interesting find. I have only filtered peptides when did 20 vials at once and I knew the needle will dull.

Looks like it's not compatible

Tisch Scientific | Material Compatibility

www.tischscientific.com

Every AI search I do tells me its not compatible or caution advised.

But since bac water is .9% BA, maybe its fine?

If I do start filtering my peptides I will probably use hydrophilic pvdf filters that have 10% or less protein binding and are BA compatible , compared to pes that bind up to 5% but are not BA compatible.

 

[Chrispy62]

Every AI search I do tells me its not compatible or caution advised.

But since bac water is .9% BA, maybe its fine?

If I do start filtering my peptides I will probably use hydrophilic pvdf filters that have 10% or less protein binding and are BA compatible , compared to pes that bind up to 5% but are not BA compatible.

Good find. But searching it up, it seems to be more an issue for degradation of the filter itself, so using it one time should be fine? Ive just seen any reconstitution with filtering video for peptides advise pes

 

[V.primal]

Good find. But searching it up, it seems to be more an issue for degradation of the filter itself, so using it one time should be fine? Ive just seen any reconstitution with filtering video for peptides advise pes

Could be just degradation over time or with higher percentage of BA, I have no idea.

Let say 1% BA isn't compatible with pes filters, it's always possible to just add it back in after filtering the peptides.

Reconstitute a few vials with WFI, let's say 7-10 day supply. Filter into a sterile vial, then add 1% pre-filtered BA back in

 

[Spaceman Spiff]

Every AI search I do tells me its not compatible or caution advised.

But since bac water is .9% BA, maybe its fine?

If I do start filtering my peptides I will probably use hydrophilic pvdf filters that have 10% or less protein binding and are BA compatible , compared to pes that bind up to 5% but are not BA compatible.

You're completely right.

It may be fine

 

[miksu7787]

@Ghoul

Membrane fouling in sterile filtration of recombinant human growth hormone - PubMed

The most troublesome problem encountered during the sterile filtration of protein solutions is membrane fouling. This article presents our study on sterile filtration of a model protein, recombinant human growth hormone (rhGH). Scanning electron microscopy (SEM) analysis shows that 0.22-mum...

pubmed.ncbi.nlm.nih.gov

Investigation on fouling mechanisms for recombinant human growth hormone sterile filtration - PubMed

During sterile filtration of recombinant human growth hormone solutions, severe membrane fouling was experienced compared to other protein preparations of significantly higher molecular weights and concentrations. This phenomenon was attributed to rhGH aggregation/adsorption occurring in the...

pubmed.ncbi.nlm.nih.gov

I entirely agree with you in terms of not using unfiltered peptides.

I was just wondering aloud whether there was any evidence of reduced efficacy (due to neutralizing antibodies) or long-term baseline IGF-1 reduction in those who have used unfiltered HGH, I suppose.

It doesn’t seem we have an epidemic of users having to take huge doses. Although I definitely have seen that with GLP-1s.

Would be a pleasure to talk with you in private messages. Same things on my mind

 

[Sera]

Would be a pleasure to talk with you in private messages. Same things on my mind

Feel free to post your thoughts, you are too new to have dm’s active anyway.

 

[brutalbuilt]

hgh is a fragile peptide you will damage it trying to filter it