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HPLC and I just buy or make references.

You'd need a special chiral column and those are expensive, short lasting and major pain in the ass generally.

For chiral specific compounds separation (not sure what you mean mechanic or enzymatic, not aware of such separation processed) is not really used on industrial level, it's mostly just synthetized that way from scratch, scratch being something optically pure, when possible AFAIK. I'm not a chem engineer though, so take me with a grain of salt.

I think I saw some articles about tren enantiomer activity, but have no sources on hand and too busy to look.

 

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