A real environment

[master.on]

Ideally your glovebox should be maintained in a negative pressure (i.e., a vacuum).

Yeah i was wondering about that. Would that need to be a constant value that was held? I was thinking about a computer fan outside of a resperator filter that would constantly pull air from the box. Then putting a filtered vent on the opposite end. My thought was if the fan pulled more air then the vent allowed make up air it would create a vacum but also filter any air that was in the box before sealing it?

To verify it's at a constant value, you would really need something to monitor differential pressure to verify that the vacuum was sufficient. That would be overkill in your case. Really all you need is for the pressure inside the box to be lower than atmospheric. I cant say for sure, but I highly doubt a computer fan would be able to provide sufficient vacuum. I would rig some 3" diameter duct work to the box (airtight sealed to the inside of the box) and a larger fan with some sort of filter (ideally a HEPA filter) to prevent debris from reaching the fan.

You actually need POSITIVE pressure
i.e. higher than atmospheric pressure
so the air leaking out prevents outside air from getting in.

Be aware it only applies if you can provide it with air perfectly filtered clean
if not, you're actually "inviting" more contaminants in
Hence my advise for neutral pressure, filtered vent port optional.

 

[Bumaga]

You actually need POSITIVE pressure
i.e. higher than atmospheric pressure
so the air leaking out prevents outside air from getting in.

Be aware it only applies if you can provide it with air perfectly filtered clean
if not, you're actually "inviting" more contaminants in
Hence my advise for neutral pressure, filtered vent port optional.

Any one that says the jacked community is full of meat heads deserves to be punched in the fucking face. I have read more insightful well thought out shit on so many subjects here. I am blown away from biology to chemistry to cyber security, eroneous laws, ethical distribution and consumption, heathy alternatives and maintaining peak performance. The Meso community continues to impress. Valid point Master.on! The fact is with exception to dr. jim every one that has replied to one of my rudimentary questions has shown patience, intelligence, and logic. THIS IS A VALID SOURCE OF KNOWLEDGE!!!

 

[Engineer]

You actually need POSITIVE pressure
i.e. higher than atmospheric pressure
so the air leaking out prevents outside air from getting in.

Be aware it only applies if you can provide it with air perfectly filtered clean
if not, you're actually "inviting" more contaminants in
Hence my advise for neutral pressure, filtered vent port optional.

Sorry-i wont argue with you here, but you are simply incorrect. You need a negative pressure inside the box-it needs to be maintained in a vacuum. The box should be airtight so that you dont have air coming in from the outside from anywhere but your ventilation rigged to the glovebox-which ideally wouldnt even be air but rather some inert gas such as argon. So, you will be controlling both the gas going in and the gas going out. This is the ideal way to control your environment. Positive pressure would require you to have a super good filter system to ensure that the pressurized air you’re pumping in is contaminate free-keeping in mind that no filter design is 100% efficient even if the micron size rating is correct.

 

[Bumaga]

Ok so this makes sense what i am wondering is if the box is in a home and the home is clean. The materials that are put into the cleaned box are clean and the air is filtered what kind of contaminents are we talking about?With precautionary steps taken along this process as best as reasonabily possible outside of a lab are these measures not effective. When you say filters are only so good, are they not good enough to prevent comtaminants that would exist after all these steps were taken?

 

[Engineer]

Ok so this makes sense what i am wondering is if the box is in a home and the home is clean. The materials that are put into the cleaned box are clean and the air is filtered what kind of contaminents are we talking about?With precautionary steps taken along this process as best as reasonabily possible outside of a lab are these measures not effective. When you say filters are only so good, are they not good enough to prevent comtaminants that would exist after all these steps were taken?

Your original post asked for advice on the “perfect” setup, so that’s what I provided. You really don’t need all those bells and whistles for a small operation at home, though. All you really need is a clean space to work in, turn off any fans/ventilation in the area, keep untopped vials covered until it’s absolutely necessary to expose them, and a venthood would be really nice

 

[Bumaga]

I mean some would argue this is extreme and that none of this is necessary. My original thought was if I were to make my own ped's how might I do so in a proffesional manner. The whole reason for brewing is to utilize qear known to be what it is supposed to be without any suprises or disappointments.

 

[Bumaga]

Your original post asked for advice on the “perfect” setup, so that’s what I provided. You really don’t need all those bells and whistles for a small operation at home, though. All you really need is a clean space to work in, turn off any fans/ventilation in the area, keep untopped vials covered until it’s absolutely necessary to expose them, and a venthood would be really nice

Yes you are right and I do appretiate both of your input.

 

[Engineer]

I mean some would argue this is extreme and that none of this is necessary. My original thought was if I were to make my own ped's how might I do so in a proffesional manner. The whole reason for brewing is to utilize qear known to be what it is supposed to be without any suprises or disappointments.

You certainly don’t need any of that fancy stuff or even a glovebox, but you really are just trying to keep dust and other airborne particulates out.
Edit-and hair lol

 

[Bumaga]

You both have illustrated that as a home brewer we can get pretty close to perfect at home with materials easily obtainable.

 

[Johne71]

You both have illustrated that as a home brewer we can get pretty close to perfect at home with materials easily obtainable.

I think I spent less than $100 in supplies for my own personal use. (Small scale, 50ml graduated cylinder, small set of beakers, glass stirring rods, grocery stores grape seed oil, 10 pre-sterilized vials). When I ordered my syringes and needles, I got some freebies. A couple of Whatman filters and two 60cc syringes. The BA and BB was pretty inexpensive too.

J

 

[master.on]

Sorry-i wont argue with you here, but you are simply incorrect. You need a negative pressure inside the box-it needs to be maintained in a vacuum. The box should be airtight so that you dont have air coming in from the outside from anywhere but your ventilation rigged to the glovebox-which ideally wouldnt even be air but rather some inert gas such as argon. So, you will be controlling both the gas going in and the gas going out. This is the ideal way to control your environment. Positive pressure would require you to have a super good filter system to ensure that the pressurized air you’re pumping in is contaminate free-keeping in mind that no filter design is 100% efficient even if the micron size rating is correct.

Not meant to argue either but positively, you need positive pressure

they ask themselves What happens if a leak arises?
If it's CDC reasearching Anthrax, a positive pressure would be deadly, as positive pressure actually pushes anthrax-spores loaded air to the researchers, like a deflating tire or balloon.
bottomline, negative pressure is only used when you want to avoid dangerous chemicals that might kill you or harm you. Gear ain't harmful, you even inject it (protecting the person)

Now imagine a super sensitive camera sensor for a military spy satellite
even an airbone speck can ruin it
positive pressure pushes and keeps contaminants outside of the glovebox.
bottomline, you want positive pressure to keep sensitive embodiments or chemicals from contamination (protecting the process)

Please check what this glovebox manufacturer has to say
What's the difference between positive and negative pressure glove boxes? | Inert

Again, not meant to argue, just to clarify.

 

[Engineer]

Not meant to argue either but positively, you need positive pressure

they ask themselves What happens if a leak arises?
If it's CDC reasearching Anthrax, a positive pressure would be deadly, as positive pressure actually pushes anthrax-spores loaded air to the researchers, like a deflating tire or balloon.
bottomline, negative pressure is only used when you want to avoid dangerous chemicals that might kill you or harm you. Gear ain't harmful, you even inject it (protecting the person)

Now imagine a super sensitive camera sensor for a military spy satellite
even an airbone speck can ruin it
positive pressure pushes and keeps contaminants outside of the glovebox.
bottomline, you want positive pressure to keep sensitive embodiments or chemicals from contamination (protecting the process)

Please check what this glovebox manufacturer has to say
What's the difference between positive and negative pressure glove boxes? | Inert

Again, not meant to argue, just to clarify.

It could work either way, it just depends on design. A big factor in design would be the chemicals or materials you’re working with, like you stated. A well-controlled environment (with an inert gas such as argon) shouldn’t have airborne particulates anyway. And a negative pressure shoukd keep the box pretty well evacuated. I’ve seen all kinds of gloveboxes for all kinds of things-but in my experience when quality control is an issue the preferred approach is negative pressure with a well-controlled inert environment