Couldn't you buy a 4x4 grow tent, a 4" can fan to exhaust the room, and cut a hole in the side and duct tape a few Med grade hepa filters to the wall. It would be a negative pressure room that exchanges the air volume in a matter of minutes. You could even put a plastic table and plastic chair in there with a fluorescent light and do your whole brew in there. Everything can be sanitized with a bleach/water mix too.
MESO-Rx
Anabolic Steroids
DIY sterile box for filling vials
- Thread starter Samael
- Start date
tenpoundsleft
Member
Exactly
And you should only need a fairly small level of positive pressure, not 300 CFM or more. 300CFM is a basic kitchen fan at full blast. I'm sure a sub-100CFM fan would be enough. You're not dealing with keeping a sandstorm out, just tiny floating specks of dust etc.
Yeah, but hepa filter got some resistance so you need more CFM to push air through it otherwise there is not enough airlow to make it work.
depends how is hepa filter rated
I've put 4x4 grow tent in google and all I got was weed pictures heh.
id rather go with simple box with holes for hands or laminar flow with positive air,
you can do whole homebrew process in there, quite handy.
this would be design:
A very primitive version of this in horizontal version:
master.on
New Member
And who's gonna clean all that? You?
Do you realize how difficult to keep a clean-room clean is?
A tent? Really?
Will it withstand alcohol to sanitize it?
If it does, How many days will the alcohol take to dry?
master.on
New Member
Aim for success!
tenpoundsleft
Member
And who's gonna clean all that? You?
Do you realize how difficult to keep a clean-room clean is?
A tent? Really?
Will it withstand alcohol to sanitize it?
If it does, How many days will the alcohol take to dry?
And who's gonna clean all that? You?
Do you realize how difficult to keep a clean-room clean is?
A tent? Really?
Will it withstand alcohol to sanitize it?
If it does, How many days will the alcohol take to dry?
You're not building nanometer electronic wafers or glass etc.
The question began in the context of keeping floating lint from getting into vials, I think you're getting carried away.
Low positive pressure and good filters ought to do it.
Yup, want to keep it simple and thats the reason behind it, as you mentioned.
Altho im really not sure how that particle got into sealed sterile vial, its obviously bigger then 0.2u so it must have been in the vial before?
Supposedly sterile vials bought from medical lab supplies uk
its a grow tent. They are made for years of abuse. All you have to do is spray it down, wipe it off and turn the fans on. I used to clean mine down every 9 weeks.And who's gonna clean all that? You?
Do you realize how difficult to keep a clean-room clean is?
A tent? Really?
Will it withstand alcohol to sanitize it?
If it does, How many days will the alcohol take to dry?
janoshik
Subscriber
You are on the right track now man, keep it up.
looks like I've found the right hepa filter:
http://www.airfiltersdelivered.com/24x24x12-commercial-hepa-filter/24-x-24-x-11-1-2-hepa-filter-99-99-standard-capacity.html
its 99.9% 0.3 micron rated, with 1 inch resistance, 1000cfm rated
http://www.airfiltersdelivered.com/24x24x12-commercial-hepa-filter/24-x-24-x-11-1-2-hepa-filter-99-99-standard-capacity.html
its 99.9% 0.3 micron rated, with 1 inch resistance, 1000cfm rated
master.on
New Member
I've put 4x4 grow tent in google and all I got was weed pictures heh.
id rather go with simple box with holes for hands or laminar flow with positive air,
you can do whole homebrew process in there, quite handy.
this would be design:
A very primitive version of this in horizontal version:
looks workable, just keep in mind
1 you may want to chemically treat wood to be absolutely certain that no mold can work on it, ever.
Not to scare but I've read some tiny spores can still get past 0.22 um filters.
2 you may need to apply a coating that resists sanitizing chemicals, at least alcohol and preferably formaldehyde.
3 don't overdo positive airpressure (fan speed/volume)
focus on great filtering instead
otherwise you'd just speed bringing particles (including bacteria mold and spores) inside.
Personally I don't use any fan, but attach true lab-grade filters to vent ports.
master.on
New Member
Still don't overwhelm yourself
brush-on wood treatments and coatings work perfectly fine.
I would probably cover interior walls with aluminum sheets, its got b-good resistance with isopropyl alcohol.
I will be not using wood material.
Id go with plexi covered with alu sheets.
You need fan powerful enough to get air inside hepa filter and blow clean one outside.
Found one in uk , rated 99.97 , 0.3 micron, and Its rated for 850 m3/h at 200pa, so i need fan around those specs, otherwise its waste of time
master.on
New Member
Make sure Al foil doesn't move around as it'll make a mess.
Time to review chemical resistance:
Lexan (polycarbonate)
Ethyl alcohol B2-Good
Isopropyl alcohol A2-Excellent
Formaldehyde A2/A1 excellent
Polycarbonate Chemical Compatibility Chart
Acrylic
Ethyl alcohol G/E Good
BA N Not recommended
Isopropyl alcohol F some damage after 7 days CONSTANT exposure
Formaldehyde E/G Excellent/good
Chemical Resistance Properties of Plexiglass Plexiglas Lucite Acrylite Acrylic plastic
So it may be better to clean with water/mild soap and sanitize with FORMALDEHYDE instead of BA/Ethanol/Isopropyl alcohol as it's milder on plastics
and sanitizes better. It's the stuff they use for surgery rooms.
Or just use a (glass) aquarium
- It's made of glass = chemical resistant
- glass = harder to scratch, will not yellow
- they are designed and tested to be leak free (at least for a few years)
- the only problem is going to be delivery. Either you'll need a large truck/van/back-seat or pay the home delivery fee.
You can see if a glass/mirrors shop can cut a front glass with cutouts for your arms, vent ports, and sell some hinges or frame for easy opening.
And they deburr all rough edges from cutouts.
If asked say it's an easy-open glass-top with cutouts for custom lights, filters and other hoses. Zero unwanted attention.
Something like this, just with additional cutouts for ventilation ports
Oh, I'd never attempt to cut glass myself.
Mechanics
New Member
That looks like a sandblasting box. I know you can buy just the box for under $500 and it's clean when bought new . just have to change the gloves to some that are thinner and easier to move around with . and the attach your home made filters/fans.
This pro unit costs around 2500$ but it does the same thing as DIY version for couple of 100 bucks.
master.on
New Member
Oh I forgot to mention make sure your (long) gloves can withstand sanitizing fluids Ethyl Alcohol, Isopropyl Alcohol or Formaldehyde.
Don't use BA as sanitizing fluid, it takes forever to dry.
Nitrile
Ethyl Alcohol 3=Doubtful
Isopropyl Alcohol 2=Fair
Forlmaldehyde 3=Doubtful
Neoprene
Ethyl Alcohol 1=Satisfactory
Isopropyl Alcohol 2=Fair
Formaldehyde 3=Doubtful
Rubber Chemical Resistance Chart, Rubber Compatibility Chart - Mykin Inc
OTOH you'll just briefly wipe your gloves clean so they do withstand sanitizing chemicals.
Remember long, elbow-long gloves or longer are recommended.
I dont want to mess with glass.
I think I will go with polypropylene sheets.
They got excellent chemical resistance and i can get them here for reasonable price
Polypropylene Chemical Compatibility Chart
