Filtering and filling oil into open vials

[El Tiburon]

I'm making my first test e homebrew and I have a few questions

1) What size bottle top filter should I use and vacuum pump

2)How do I transfer the filtered oil into a OPEN vial as sterile as possible?

Your advices would be appreciated.

 

[Stevie_min]

1) Depends on your volume. You want to fit your fluid. Flow rate is directly proportional to vacuum pressure and to filter area. 500ml bottle top filters are a good choice. 250ml is often just too small.
You may use Cellulose Acetate, Nylon, or PTFE. You may not use PES, PVDF, or Glass Microfiber. All 0.22um only.

2) Use a laminar flow hood if you have access to a lab. If not, work in a clean area next to an open flame. The open flame creates a convection air current that moves air up and away, reducing the flow of air into your clean vials. Try to not touch the syringe you use to transfer to the edges of any containers. See the following for guidance: Basic Practical Microbiology: A Manual

 

[burkeen]

1) Depends on your volume. You want to fit your fluid. Flow rate is directly proportional to vacuum pressure and to filter area. 500ml bottle top filters are a good choice. 250ml is often just too small.
You may use Cellulose Acetate, Nylon, or PTFE. You may not use PES, PVDF, or Glass Microfiber. All 0.22um only.

2) Use a laminar flow hood if you have access to a lab. If not, work in a clean area next to an open flame. The open flame creates a convection air current that moves air up and away, reducing the flow of air into your clean vials. Try to not touch the syringe you use to transfer to the edges of any containers. See the following for guidance: Basic Practical Microbiology: A Manual

Why not PES or PVDF?

 

[MDrabbit]

The solvents will desolve minor amounts of the filter and you will end up with filter material in the end product.

 

[MDrabbit]

For small brew quantities it is best to use a glass filtration system with a PTFE filter.

 

[Bigguy81]

1) Depends on your volume. You want to fit your fluid. Flow rate is directly proportional to vacuum pressure and to filter area. 500ml bottle top filters are a good choice. 250ml is often just too small.
You may use Cellulose Acetate, Nylon, or PTFE. You may not use PES, PVDF, or Glass Microfiber. All 0.22um only.

2) Use a laminar flow hood if you have access to a lab. If not, work in a clean area next to an open flame. The open flame creates a convection air current that moves air up and away, reducing the flow of air into your clean vials. Try to not touch the syringe you use to transfer to the edges of any containers. See the following for guidance: Basic Practical Microbiology: A Manual

Don't listen to this brother you cannot use CA or PES filters. You can use hydrophobic PVDF, and PTFE. Technically you can also use nylon, which are only hydrophilic therefore they suck because it will be slow as hell to filter. Just make sure you pre wet your hydrophobic filters first.

 

[JackSkel]

The solvents will desolve minor amounts of the filter and you will end up with filter material in the end product.

If your solvents are dissolving those filters then you’re using WAY too much solvent.

 

[Bigguy81]

If your solvents are dissolving those filters then you’re using WAY too much solvent.

Not true brother. Even small amounts of solvent such as 10% BB or 10% EO will dissolve a PES membrane when in contact for too long and cellulose acetate cannot be used at all. If you have any literature Stating otherwise I would love to see it, as i have plenty of literature stating that you cannot use PES or CA membranes as they are not chemically compatible

 

[burkeen]

Not true brother. Even small amounts of solvent such as 10% BB or 10% EO will dissolve a PES membrane when in contact for too long and cellulose acetate cannot be used at all. If you have any literature Stating otherwise I would love to see it, as i have plenty of literature stating that you cannot use PES or CA membranes as they are not chemically compatible

Thanks man, found that pes and nylon only has Limited expoture to BA, not a risk i want to take

 

[burkeen]

Don't listen to this brother you cannot use CA or PES filters. You can use hydrophobic PVDF, and PTFE. Technically you can also use nylon, which are only hydrophilic therefore they suck because it will be slow as hell to filter. Just make sure you pre wet your hydrophobic filters first.

"Hydrophobic membranes, like our PTFE membranes, can be pre-wetted using a liquid with low surface tension, such as 100% isopropanol or the 60:40 IPA solution. Submerge and rotate the element in the liquid for approximately 30 seconds to ensure proper wetting."

How do you wet a capsule filter ?
When you do i guess you use BA insted ?

 

[Zebedee]

The absolute vast majority of sterile Nylon / PVDF / PTFE bottletops I’ve seen have polystyrene filter housings / funnels, with polystyrene usually being listed as incompatible with BB.

Have any of you guys ever personally experienced problems from BB with such housings / funnels?

 

[Denicnut2427]

If your solvents are dissolving those filters then you’re using WAY too much solvent.

1% ba and 18%bb work good for almost everything and in normal concentrations…lot of the time you can get away with less bb too really…my dhb I do 1%ba and 20%bb it hold easy at 75mg per ml and at 100mg per ml it crashes if sitting for too long and cooler temp rooms and my 250mg mast enth I do 20%bb…test enth I do 300mg per ml and have gotten it down to like 12%bb with no problems and fucking smooth! EQ and deca too maybe little bit more

 

[Denicnut2427]

Not true brother. Even small amounts of solvent such as 10% BB or 10% EO will dissolve a PES membrane when in contact for too long and cellulose acetate cannot be used at all. If you have any literature Stating otherwise I would love to see it, as i have plenty of literature stating that you cannot use PES or CA membranes as they are not chemically compatible

I believe when I researched it said the same not to use PES…I get good filters too it’s worth it to get the whaatman syringe filters

 

[helionn]

The absolute vast majority of sterile Nylon / PVDF / PTFE bottletops I’ve seen have polystyrene filter housings / funnels, with polystyrene usually being listed as incompatible with BB.

Have any of you guys ever personally experienced problems from BB with such housings / funnels?

Nalgene Nylon Bottle Top filters melted with 2/20. Ruined a L brew with it. Oil got really cloudy. Threw out all the rest of them and bought a glass setup using filter paper. No issues since and filters a L of mig840 in 15 mins ish or 30-45 mins with GSO.

 

[narta]

Play it safe: PTFE hydrophobic or PVDF. Nylon on absolute emergency. PES and MC is shit for our purposes. And replace the receiver flask with a glass one, better pressure resistance and no dissolving of the housing.

 

[burkeen]

Nalgene Nylon Bottle Top filters melted with 2/20. Ruined a L brew with it. Oil got really cloudy. Threw out all the rest of them and bought a glass setup using filter paper. No issues since and filters a L of mig840 in 15 mins ish or 30-45 mins with GSO.

Are we talking about this funnel ?

 

[nightprowler7]

The absolute vast majority of sterile Nylon / PVDF / PTFE bottletops I’ve seen have polystyrene filter housings / funnels, with polystyrene usually being listed as incompatible with BB.

Have any of you guys ever personally experienced problems from BB with such housings / funnels?

With high bb, over 25% I had leeching. Yes. With a 22% brew, no leeching

 

[Zebedee]

Play it safe: PTFE hydrophobic or PVDF. Nylon on absolute emergency. PES and MC is shit for our purposes. And replace the receiver flask with a glass one, better pressure resistance and no dissolving of the housing.

Think you’ve misunderstood what I was referring to mate - I should have been clearer. I was referring to the bottletop reservoir that holds the liquid before filtering & the housing around the actual filter material itself being almost always being polystyrene.

 

[Zebedee]

Nalgene Nylon Bottle Top filters melted with 2/20. Ruined a L brew with it. Oil got really cloudy. Threw out all the rest of them and bought a glass setup using filter paper. No issues since and filters a L of mig840 in 15 mins ish or 30-45 mins with GSO.

Was the ruined brew in Mig840 or GSO?

Curious because it would seem from this current thread ….

Mig840 as a Solvent

….that Mig840 most definitely has decent solvent properties itself.

 

[narta]

Think you’ve misunderstood what I was referring to mate - I should have been clearer. I was referring to the bottletop reservoir that holds the liquid before filtering & the housing around the actual filter material itself being almost always being polystyrene.

Yeah I understood what you were pointing out that's why I said replacing the receiver flask with a glass one is the best option. The holding/filtering flask can't be replaced but one can minimize contact by adding slowly and not all at once. But as @nightprowler7 pointed out after a certain amount of bb it leaches. An all glass system is the best but you need to sterilize it before each use and that has its problems if you don't know exactly what you are.doing.