Filtering finished oils

[Jimin Marvinluder]

Apologies if I've asked a repeat question, I did try to read through the whole thread as well as I could.

Would there be any benefit to filtering before putting oils into long-term storage (say, freezing)? Or would it be better to filter as needed when removing from storage? I see how using a higher quality vial would prolong the life of the storage medium itself, just wondering if there's any other benefit.

 

[Ghoul]

Do one! Do one!

Finnrick could pound with the force of a tide,
Every moan, every gasp, he’d provide.
He’d grind, twist, and squeeze,
Bring every chick to her knees,
All thanks to a special peptide....

 

[Ghoul]

Apologies if I've asked a repeat question, I did try to read through the whole thread as well as I could.

Would there be any benefit to filtering before putting oils into long-term storage (say, freezing)? Or would it be better to filter as needed when removing from storage? I see how using a higher quality vial would prolong the life of the storage medium itself, just wondering if there's any other benefit.

Leave it sealed. After you thaw, heat to uncrash, filter as you transfer (not hot, just warm) into a fresh vial just before use.

 

[Sampei]

Hooker's asshole, we got it. I am a slow learner but catching up. Appreciate the patience.

You think many of these sources actually have a vertical laminar flow hood and perform proper PMs, testing?

https://www.terrauniversal.com/blog/horizontal-vs-vertical-laminar-flow-hoods

Horizontal are better for brewing steroid

 

[readalot]

Horizontal are better for brewing steroid

Really? Why? I would want particle free sterile field to prep vials. ??

 

[Sampei]

Really? Why? I would want particle free sterile field to prep vials. ??

Because 99% of home brewers don't have a work bench with holes so the ground of your workbench gets turbulent and you need to be very careful in not contaminating your hands on anyway either or you gonna push the contamination into open vials or open media bottles and when you move you need to keep your hands always lower and never above things to not disrupt the flow of air.

Vertical laminar flow are superior for any preparations that needs to protect the operators from dangerous contaminations. As we don't care in this case of the safety of the operator because we don't brew anything that can be toxic etc. we care only about maximum efficiency and absolute sterility, and we don't use any big instruments that would stop the flow of the hood being placed on the bench, horizontal flow keeps the environment more easily sterile.

 

[Sampei]

Horizontal flow hoods excel in product protection through consistent first air delivery. The horizontal airflow travels parallel to the work surface, minimizing turbulence and providing uniform HEPA-filtered air coverage across all materials. This configuration allows sterile materials to be positioned closer to the filter face, upstream from potential contaminants.

Vertical flow hoods, while providing good product protection, create higher turbulence when air strikes the work surface perpendicularly. This can potentially increase contamination risk if not properly managed through appropriate aseptic technique

 

[Ghoul]

Because 99% of home brewers don't have a work bench with holes so the ground of your workbench gets turbulent and you need to be very careful in not contaminating your hands on anyway either or you gonna push the contamination into open vials or open media bottles and when you move you need to keep your hands always lower and never above things to not disrupt the flow of air.

Vertical laminar flow are superior for any preparations that needs to protect the operators from dangerous contaminations. As we don't care in this case of the safety of the operator because we don't brew anything that can be toxic etc. we care only about maximum efficiency and absolute sterility, and we don't use any big instruments that would stop the flow of the hood being placed on the bench, horizontal flow keeps the environment more easily sterile.

I think the joke didn't translate very well

 

[readalot]

Because 99% of home brewers don't have a work bench with holes so the ground of your workbench gets turbulent and you need to be very careful in not contaminating your hands on anyway either or you gonna push the contamination into open vials or open media bottles and when you move you need to keep your hands always lower and never above things to not disrupt the flow of air.

Vertical laminar flow are superior for any preparations that needs to protect the operators from dangerous contaminations. As we don't care in this case of the safety of the operator because we don't brew anything that can be toxic etc. we care only about maximum efficiency and absolute sterility, and we don't use any big instruments that would stop the flow of the hood being placed on the bench, horizontal flow keeps the environment more easily sterile.

Thank you for your detailed comments. I am pondering all this.

 

[readalot]

I think the joke didn't translate very well

I completely missed it if there was one. Losing a few steps.

 

[readalot]

Horizontal flow hoods excel in product protection through consistent first air delivery. The horizontal airflow travels parallel to the work surface, minimizing turbulence and providing uniform HEPA-filtered air coverage across all materials. This configuration allows sterile materials to be positioned closer to the filter face, upstream from potential contaminants.

Vertical flow hoods, while providing good product protection, create higher turbulence when air strikes the work surface perpendicularly. This can potentially increase contamination risk if not properly managed through appropriate aseptic technique

Great comments. I read the entire article I linked another 3 times.

Yet even a solid top may not rule out vertical flow if work is performed above, not on, the work surface. If sterile or particle-sensitive processes are performed in a clean, sterile zone midway between the work surface and the filter face, a vertical flow hood is generally acceptable. As long as hands and other contamination sources move up and down, not sideways above a sample, sensitive materials will remain clean.

Not arguing, but if floor space at a premium I think I am going with vertical assuming person understands aseptic technique and maintaining sterile field. Also to your point consider height of working surface. Good point about air flowing orthogonal to vial entrance with horizontal flow hood.

Appreciate your summary.

 

[Sampei]

Great comments. I read the entire article I linked another 3 times.



Not arguing, but if floor space at a premium I think I am going with vertical assuming person understands aseptic technique and maintaining sterile field. Also to your point consider height of working surface. Good point about air flowing orthogonal to vial entrance with horizontal flow hood.

Appreciate your summary.

They both work fine anyway, considering almost no one use it and we don't have any abscess epidemic around I believe just having one is already 1000% better than not having it

 

[readalot]

They both work fine anyway, considering almost no one use it and we don't have any abscess epidemic around I believe just having one is already 1000% better than not having it

Hey we are working on it. OEP thread is getting long and we barely got through the stoppers. Nice clinic on sterile vs clean you just did.

 

[Sampei]

Hey we are working on it. OEP thread is getting long and we barely got through the stoppers. Nice clinic on sterile vs clean you just did.

Trying my best to navigate this fascinating world, learning every day something new

 

[explodingHeart]

Leave it sealed. After you thaw, heat to uncrash, filter as you transfer (not hot, just warm) into a fresh vial just before use.

Is it worth buyign a cheap pressure cooker to try and hit 121c for 30 min after filtering my UG chinese test-c into a new vial?


Vial is rated for autoclaves apparently and the stopper can handle the heat.

Plan:
- Filter 20ml of UG test-c into 30ml vial
- Suck out the air and leave a vacuum
- 30 minutes in pressure cooker at 12-15psi

Goal:
- Kill some bacteria making it a bit more safe

 

[readalot]

Goal:
- Kill some bacteria making it a bit more safe

That's what the 0.2 um filtration is for.

R

Post in thread 'Filtering finished oils'

Aug 13, 2025

Oh ok. I see where folks are at.

0.2 um filtration will sterilize the gear. Terminal sterilization ensures endotoxins are taken care of in a pharma product. We spent quite a bit of time measuring endotoxins in gear and the risk appears remote.

Me? I will just use the 0.2 um filter and call it a day. For those with lower risk tolerance they can do the TS (dont do this with tren, lol!!!).

0.2 um filtration will remove the bacteria. Look up 0.1 vs 0.2 um filtration for more fun.

• readalot

• 

 

[explodingHeart]

That's what the 0.2 um filtration is for.

Yes but the conclusion was 0.2 missed a lot of bacteria.

 

[Photon]

Yes but the conclusion was 0.2 missed a lot of bacteria.

Please share the link

 

[readalot]

Yes but the conclusion was 0.2 missed a lot of bacteria.

??
Whose conclusion?

0.2 um doesn't filter pyrogens / endotoxins. I spent enough time and $$ ruling that out to my satisfaction.

 

[explodingHeart]

??
Whose conclusion?

0.2 um doesn't filter pyrogens / endotoxins. I spent enough time and $$ ruling that out to my satisfaction.

You guys are the experts on this so I'll take your word for it. It's not worth it even if it would be a minor expense to get a pressure cooker?