Getting filtered brew from the Media Storage Bottle into a pre-sterilized vial?

[lebroseixoife-6939]

I am wondering how do folks get their filtered brew out of the Glass Round Media Storage Bottles with GL45 Screw Cap into a pre-sterilized vial without breaking sterilization after you run it through the bottle filter?

 

[qadsan]

Here’s a few ways I can think of…

1) Do it under a laminar flow hood. Or…

2) Make sure your GL45 has a septum on it and draw from the septum, then inject into the sterile vial. Or…

3) Draw from the GL45 in the cleanest environment you have wearing a mask and inject into the sterile vial through a 0.22um sterile syringe filter.