Got an autoclave

[Benny]

picked up my last piece of the puzzle for my home brewing, shown here warming up running a test cycle. Being new to this, I want to make sure my thought process is right. My plan is to mix up my brew, heat and stir until clear, filter, place in properly prepped vials, install the sterilized caps and stoppers, and then run them in autoclave. It's my understanding that when pressure cooking like that I do not have to vent the vials. Any advice before I start is always appreciated.

 

[Opti]

Don't have much experience regarding autoclaves but WOW, she is a beauty! Congrats! How much did it set you back?

 

[Benny]

It was $400. Guy sold it cheap because there was a water level error code preventing it from working. Rubbed down the water level sensor with my finger tip and it runs like a champ.

 

[Opti]

It was $400. Guy sold it cheap because there was a water level error code preventing it from working. Rubbed down the water level sensor with my finger tip and it runs like a champ.

LOL, excellent! Happy sterile brewing Benny.

 

[mghoward74]

Congrats

I'm waiting in line.....my ticket # is 001....

Just kidding....good luck and check out basskiller online for any questions

 

[Docd187123]

You do NOT vent vials when autoclaving. Otherwise you got the process down basically n

 

[Benny]

You do NOT vent vials when autoclaving. Otherwise you got the process down basically n

Thanks doc. I was thinking that was proper, but didn't want to blow the caps off my first batch for lack of asking.

 

[Benny]

Ended up with a rather free evening last night, so set up and ran my first brew. Have it all brewed and in vials now, will autoclave the vials tonight. Plan to lab max and pin it later this week.
250ml of NPP in GSO, 2% BA, 18% BB, 100mg/ml.

 

[Docd187123]

Looks good.

 

[fightingfires]

when in doubt.....you can always fill a vial with just carrier oil and BA + BB and run it.
good to practice and will tell you everything you need to know

 

[6Fingers-Pharma]

View attachment 23912 picked up my last piece of the puzzle for my home brewing, shown here warming up running a test cycle. Being new to this, I want to make sure my thought process is right. My plan is to mix up my brew, heat and stir until clear, filter, place in properly prepped vials, install the sterilized caps and stoppers, and then run them in autoclave. It's my understanding that when pressure cooking like that I do not have to vent the vials. Any advice before I start is always appreciated.

Let me see if I understand this correctly: You are going to place filled vials in an autoclave and run the cycle?

Please tell me you did not do this

 

[6Fingers-Pharma]

You don't autoclave after you fill vials. Your gear will be ruined, likely blowing the caps and stoppers out like bullets!!!
WTF?!
Tell me I am misreading it because I keep reading hoping it says something different

 

[6Fingers-Pharma]

Autoclaving should be done to sterilize your open vials/butyl-stoppers. You do not autoclave media. BA and filtration takes care of all the sterilization the product needs.

 

[Benny]

You don't autoclave after you fill vials. Your gear will be ruined, likely blowing the caps and stoppers out like bullets!!!
WTF?!
Tell me I am misreading it because I keep reading hoping it says something different

Why would you blow the tops off like bullets? The whole idea behind the autoclave is pressure with the heat. That pressure works against the pressure in the vials to equalize it and keep the tips from blowing off or leaking. But them in an oven, and yes you will have problems because the pressure rise in the vial will be much greater the the unpressurized air around it.

 

[colossus25]

Autoclaving should be done to sterilize your open vials/butyl-stoppers. You do not autoclave media. BA and filtration takes care of all the sterilization the product needs.

Who are you? Lol

 

[colossus25]

You joined like a day ago....

 

[fightingfires]

Have you ever run an autoclave 6fingers? I personally have not. Though upon looking up autoclave temps it is similar to pressure cooking in that it heats to 249F. Maybe there are more than just that setting but at that setting it will not blow the caps and stoppers off. Simply vent the stopper with a pin. Just make sure the pins lowest point is still above the oil level. OP made NPP which has a melt point of 197-204F so it is VERY doubtful that any of the gear would be "ruined" as you suggested. Guys have been pressure cooking gear for a long time with no problems. Obviously different hormones may react differently but NPP should be fine. I've baked before...never beyond 200F though but my gear did not suffer in any way according to the bloods that came back from running it.

 

[6Fingers-Pharma]

So if the vials are sealed, what is the point of autoclaving?

 

[6Fingers-Pharma]

I'm a fucking manufacturer in China. I've run a few sterilizers in my fucking day. OP is filling vials and sealing them. Then attempting to autoclave

 

[6Fingers-Pharma]

Put pins in them and it'll be okay? Where do you think the heat comes from? Steam. You ever get water in gear?