Injectable Glutathione Recipe

[Pez'sAnabolicJunta]

I really struggled to get it right and I wanna save people looking for the recipe some time so they don't have to go through all posts.

This is the recipe for 30 ml at 200 mg/ml:

1) Weigh out 6 grams of Reduced Glutathione powder and pour it in your mixing beaker.
2) Add around 20 ml of Bacteriostatic water and start mixing (I prefer magnetic stirrer).
3) After a couple of minutes a milky cloudy solution will form. To get it to completely dissolve you will need to increase te pH of the solution.
4) Add NaOH pellets or NaOH solution to your mix. Add it very slowly, ml by ml or a couple of mg by couple of mg. If you get the pH above 6.5 it will oxidize instantly turning red and ruining your product. Having a pH meter is a life saver. The solution will start clearing up before 4.0 but I like to keep my brew between 4.5 and 4.9.
5) Once you've achieved your desierd pH, the solution should be crystal clear.
6) Add bacteriostatic water up to 30 ml and mix for another 5-6 minutes.
6) You can now filter your solution into a vials through 0.22 micron filter (I prefer Nylon66).
7) Once you've done you should store your Glutathione. If you are going to use it in the next 1-2 months you can store it in the fridge under 5 degrees C. If you are not going to use it soon then you should put it in the freezer and unfreeze it when you are ready to use it.

Thank you for posting this. I tried to make Acetyl L- Carnitine back around 2015. Doctor at my HRT clinic thought it was a good idea too. Not that the guy tossed me a few comps for the idea as it were. What sites sell good raw supp powders and maybe some smart nutriceuticals too?

 

[nullandvoid]

Acecosm is having a sale on Memorial Day. 20% off site-wide, so their 10-vial kits of 1200mg vials of Glutathione will be $48. Additionally, I think you can stack for an extra 10% with MDAY2025 (the 20% will be automatic that day).

I've also modified my technique. And I get all this from ChatGPT, so don't think I'm some sort of scientist. But apparently using bacteriostatic NaCl isn't ideal because it accelerates degradation due to oxidation. Unless quickly used, it's pretty significant degradation after three days in the fridge. So what I do now is reconstitute and immediately empty the vials by prefilling 12 syringes and freezing them. I also pull back and leave a bit of space in the syringes to reduce potential shear stress when freezing/expanding. Then on days I use it, I take one from freezer, let it thaw in fridge, and later inject.

Grok didn't catch anything about oxidation. ChatGPT was pretty quick about it. It also stated Glutathione should not have a strong odor like I'm used to smelling. The odor actually indicates oxidation.

In spite of any oxidation or errors, the Glutaone kits did a great job when I was taking Accutane at 160mg/day. My liver values were in mid 20's at the end of a five-month course. I've since reduced to 100mg every other day.

 

[Photon]

Acecosm is having a sale on Memorial Day. 20% off site-wide, so their 10-vial kits of 1200mg vials of Glutathione will be $48. Additionally, I think you can stack for an extra 10% with MDAY2025 (the 20% will be automatic that day).

I've also modified my technique. And I get all this from ChatGPT, so don't think I'm some sort of scientist. But apparently using bacteriostatic NaCl isn't ideal because it accelerates degradation due to oxidation. Unless quickly used, it's pretty significant degradation after three days in the fridge. So what I do now is reconstitute and immediately empty the vials by prefilling 12 syringes and freezing them. I also pull back and leave a bit of space in the syringes to reduce potential shear stress when freezing/expanding. Then on days I use it, I take one from freezer, let it thaw in fridge, and later inject.

Grok didn't catch anything about oxidation. ChatGPT was pretty quick about it. It also stated Glutathione should not have a strong odor like I'm used to smelling. The odor actually indicates oxidation.

In spite of any oxidation or errors, the Glutaone kits did a great job when I was taking Accutane at 160mg/day. My liver values were in mid 20's at the end of a five-month course. I've since reduced to 100mg every other day.

You bacteriostatic NaCl is not commonly used, any specific reason why you dont just use normal bacteriostatic water?

 

[Sampei]

Acecosm is having a sale on Memorial Day. 20% off site-wide, so their 10-vial kits of 1200mg vials of Glutathione will be $48. Additionally, I think you can stack for an extra 10% with MDAY2025 (the 20% will be automatic that day).

I've also modified my technique. And I get all this from ChatGPT, so don't think I'm some sort of scientist. But apparently using bacteriostatic NaCl isn't ideal because it accelerates degradation due to oxidation. Unless quickly used, it's pretty significant degradation after three days in the fridge. So what I do now is reconstitute and immediately empty the vials by prefilling 12 syringes and freezing them. I also pull back and leave a bit of space in the syringes to reduce potential shear stress when freezing/expanding. Then on days I use it, I take one from freezer, let it thaw in fridge, and later inject.

Grok didn't catch anything about oxidation. ChatGPT was pretty quick about it. It also stated Glutathione should not have a strong odor like I'm used to smelling. The odor actually indicates oxidation.

In spite of any oxidation or errors, the Glutaone kits did a great job when I was taking Accutane at 160mg/day. My liver values were in mid 20's at the end of a five-month course. I've since reduced to 100mg every other day.

BULLSHIT

Conclusion:
Bacteriostatic NaCl does not directly accelerate the oxidation of reduced glutathione. However, high concentrations of NaCl may indirectly contribute to oxidative stress by reducing antioxidant enzyme activity, which could, in turn, favor GSH oxidation in biological systems. The direct chemical oxidation of GSH is primarily mediated by reactive oxygen species, not by NaCl itself

 

[nullandvoid]

If I use bacteriostatic NaCl, it saves me on the volume I have to use to maintain a (nearly) irritation-free injection. 11ml of bacteriostatic NaCl versus 19ml of bacteriostatic water. I have a strong dislike for extending into multiple syringes and injecting more than 1ml at a time.

 

[Photon]

If I use bacteriostatic NaCl, it saves me on the volume I have to use to maintain a (nearly) irritation-free injection. 11ml of bacteriostatic NaCl versus 19ml of bacteriostatic water. I have a strong dislike for extending into multiple syringes and injecting more than 1ml at a time.

Bac water is usually acidic.
Bacteriostatic NaCl is probably closer to neutral i guess.
I don't think it's gonna make a difference unless your pH is >7.2, which is not likely.

If you take a look at Hospira Bacteriostatic NaCl, pH is 5.0 (4.5 to 7.0), which is pretty much the same as their normal Bacteriostatic water.

https://labeling.pfizer.com/ShowLabeling.aspx?id=4614

 

[JustJoe]

Acecosm is having a sale on Memorial Day. 20% off site-wide, so their 10-vial kits of 1200mg vials of Glutathione will be $48. Additionally, I think you can stack for an extra 10% with MDAY2025 (the 20% will be automatic that day).

I've also modified my technique. And I get all this from ChatGPT, so don't think I'm some sort of scientist. But apparently using bacteriostatic NaCl isn't ideal because it accelerates degradation due to oxidation. Unless quickly used, it's pretty significant degradation after three days in the fridge. So what I do now is reconstitute and immediately empty the vials by prefilling 12 syringes and freezing them. I also pull back and leave a bit of space in the syringes to reduce potential shear stress when freezing/expanding. Then on days I use it, I take one from freezer, let it thaw in fridge, and later inject.

Grok didn't catch anything about oxidation. ChatGPT was pretty quick about it. It also stated Glutathione should not have a strong odor like I'm used to smelling. The odor actually indicates oxidation.

In spite of any oxidation or errors, the Glutaone kits did a great job when I was taking Accutane at 160mg/day. My liver values were in mid 20's at the end of a five-month course. I've since reduced to 100mg every other day.

Reduced Glutathione will always will have a sulphurous Odor, because sulpher compounds are a part of the molecule.

When glutathione or glutamine is dissolved in water, the disulfide bonds can partially break, leading to the release of hydrogen sulfide, which has a strong rotten egg smell.

The stronger the smell the more potent the concentration. The sulpher is also a feature not a problem. The sulpher attaches to free radicals in your body and then the glutathione helps flush them away.

They make glutathione that doesn’t smell for cosmetics but it is not as potent or beneficial to the body.

There are two different forms of glutathione: reduced glutathione (GSH), which is the active form, and oxidized glutathione, the inactive state. As GSH patrols the cellular environment and puts out oxidative “free radical” fires, it becomes oxidized and inactive.

 

[JustJoe]

So I did some experimenting myself tonight.. And what I've come to the realization is,

That's not 100% fact It might be with that particular brand, but I actually researched it a little bit and several brands actually do use NAOH , So I'm going to go with not every manufacturer uses the same buffer... If you actually follow a link somebody has on here to a place in China that sells 10 vials in a kit, there are instructions linked on the website and if you read them carefully it explains how to make a buffered solution with NAOH, to prepare the vials in the kit. Some kits do you not come buffered and I tracked down several places that sell the vials , because I considered buying them before I decided to make my own.

I know I’m replying to an old post but wanted to point out there’s a difference between “buffering” and adjusting the pH.

NaOH can be used to adjust the pH of a solution, but it's not a buffer the
Solution. Buffers keep solutions from swinging back and forth in pH.

NaOH is just a very strong base that neutralizes the acidic properties of the glutathione solution.

Sodium bicarbonate can be used as a buffer AND to increase the solubility of glutathione, particularly at higher concentrations.

Here's why sodium bicarbonate can act as a buffer for glutathione:

• pH buffering:
  Sodium bicarbonate is a weak base that can neutralize excess hydrogen ions (H+), effectively buffering the pH of a solution.
  
  
• Solubility:
  Glutathione can be poorly soluble in water, especially at higher concentrations. Sodium bicarbonate can help dissolve GSH, making it more suitable for use in solutions and formulations.
  
  
• Improved protein kinetics:
  Studies have shown that potassium bicarbonate, a close relative of sodium bicarbonate, can improve whole-body protein kinetics and increase glutathione availability according to a study published in ScienceDirect.
  
  
• Enhanced mucociliary transport:
  In cystic fibrosis, the combination of bicarbonate and glutathione has been shown to improve mucociliary transport, according to a study published by the NIH.

 

[JustJoe]

Also, the higher the pH of the GSH, the quicker it degrades in solution.

Here’s a great geeky read on researchgate if you’re interested:

https://www.researchgate.net/post/How_to_prepare_a_20_mM_reduced_glutathione_solution_with_pH_7#view=5e3cb8037ccd828a340ac2ab

 

[JustJoe]

Yea thats cause its cold lol

It’s more the fact that glutathione is not super soluble in water, unless it’s buffered.

 

[Mac81]

I know I’m replying to an old post but wanted to point out there’s a difference between “buffering” and adjusting the pH.

NaOH can be used to adjust the pH of a solution, but it's not a buffer the
Solution. Buffers keep solutions from swinging back and forth in pH.

NaOH is just a very strong base that neutralizes the acidic properties of the glutathione solution.

Sodium bicarbonate can be used as a buffer AND to increase the solubility of glutathione, particularly at higher concentrations.

Here's why sodium bicarbonate can act as a buffer for glutathione:

• pH buffering:
  Sodium bicarbonate is a weak base that can neutralize excess hydrogen ions (H+), effectively buffering the pH of a solution.
  
  
• Solubility:
  Glutathione can be poorly soluble in water, especially at higher concentrations. Sodium bicarbonate can help dissolve GSH, making it more suitable for use in solutions and formulations.
  
  
• Improved protein kinetics:
  Studies have shown that potassium bicarbonate, a close relative of sodium bicarbonate, can improve whole-body protein kinetics and increase glutathione availability according to a study published in ScienceDirect.
  
  
• Enhanced mucociliary transport:
  In cystic fibrosis, the combination of bicarbonate and glutathione has been shown to improve mucociliary transport, according to a study published by the NIH.

I don't know what to say about the pH swinging back and forth... I had to do a quick check tonight of my last batch after reading your post,I'm not seeing any ph fluctuation.. It's still holding at a steady 6.8 several weeks in at over 600mg/ml and is still crystal clear using nothing but NAOH.. I'm not sure what would cause pH swings but its not happening with this current recipe at all,and no bicarbonate was needed. As far as the higher the pH the lower the stability causing the half life to change.... I read about that.. I use it daily so I'm not really worried about the half-life, 9 hours Is more than enough for me using something daily. I suppose some people would want a longer half life

 

[Mac81]

I know I’m replying to an old post but wanted to point out there’s a difference between “buffering” and adjusting the pH.

NaOH can be used to adjust the pH of a solution, but it's not a buffer the
Solution. Buffers keep solutions from swinging back and forth in pH.

NaOH is just a very strong base that neutralizes the acidic properties of the glutathione solution.

Sodium bicarbonate can be used as a buffer AND to increase the solubility of glutathione, particularly at higher concentrations.

Here's why sodium bicarbonate can act as a buffer for glutathione:

• pH buffering:
  Sodium bicarbonate is a weak base that can neutralize excess hydrogen ions (H+), effectively buffering the pH of a solution.
  
  
• Solubility:
  Glutathione can be poorly soluble in water, especially at higher concentrations. Sodium bicarbonate can help dissolve GSH, making it more suitable for use in solutions and formulations.
  
  
• Improved protein kinetics:
  Studies have shown that potassium bicarbonate, a close relative of sodium bicarbonate, can improve whole-body protein kinetics and increase glutathione availability according to a study published in ScienceDirect.
  
  
• Enhanced mucociliary transport:
  In cystic fibrosis, the combination of bicarbonate and glutathione has been shown to improve mucociliary transport, according to a study published by the NIH.

Generally whatever additive they are using achieves both though if you actually read about it... And they have several different ways of doing it five that I have seen of so far, but I'm sure there is more... They might be two different things I'm not going to argue that, but they are achieving both of those things by using one thing to do it.. some are using phosphates, some we're using NAOH, some are using baking soda, none of them are perfect and they all have pros and cons, but it's all achieving both things on its own . At least that's what I can tell from everything I've read

 

[Photon]

I know I’m replying to an old post but wanted to point out there’s a difference between “buffering” and adjusting the pH.

NaOH can be used to adjust the pH of a solution, but it's not a buffer the
Solution. Buffers keep solutions from swinging back and forth in pH.

NaOH is just a very strong base that neutralizes the acidic properties of the glutathione solution.

Sodium bicarbonate can be used as a buffer AND to increase the solubility of glutathione, particularly at higher concentrations.

Here's why sodium bicarbonate can act as a buffer for glutathione:

• pH buffering:
  Sodium bicarbonate is a weak base that can neutralize excess hydrogen ions (H+), effectively buffering the pH of a solution.
  
  
• Solubility:
  Glutathione can be poorly soluble in water, especially at higher concentrations. Sodium bicarbonate can help dissolve GSH, making it more suitable for use in solutions and formulations.
  
  
• Improved protein kinetics:
  Studies have shown that potassium bicarbonate, a close relative of sodium bicarbonate, can improve whole-body protein kinetics and increase glutathione availability according to a study published in ScienceDirect.
  
  
• Enhanced mucociliary transport:
  In cystic fibrosis, the combination of bicarbonate and glutathione has been shown to improve mucociliary transport, according to a study published by the NIH.

Sodium bicarb is a much weaker base like you said.

One needs much more sodium bicarb vs naOh which results in a lower concentration of glut. As long as you constantly mix, use a continuous pH meter and ensure that the pH doesn't go >7.2, I see naOh as a superior solution.

A highly concentrated sodium bicarb has a pH of ~8.5 vs naOH which has a pH of ~15. This means you cannot dump a crap load of naOh inside at once as it causes the pH to spike and destroy your glut.

If you are fine with a lower concentration then sodium bicarb would probably work, but what some of us are aiming for is 700+ mg/ml instead of 200mg/ml of glut.

 

[Mac81]

Sodium bicarb is a much weaker base like you said.

One needs much more sodium bicarb vs naOh which results in a lower concentration of glut. As long as you constantly mix, use a continuous pH meter and ensure that the pH doesn't go >7.2, I see naOh as a superior solution.

A highly concentrated sodium bicarb has a pH of ~8.5 vs naOH which has a pH of ~15. This means you cannot dump a crap load of naOh inside at once as it causes the pH to spike and destroy your glut.

All of this I definitely agree with!! I don't think I could have said it better myself.. I'm sure more people will try different things, And we will come up with more ways to do this.. It's just interesting that everybody's coming together and what we've come up with so far

 

[Photon]

Sodium bicarb is a much weaker base like you said.

One needs much more sodium bicarb vs naOh which results in a lower concentration of glut. As long as you constantly mix, use a continuous pH meter and ensure that the pH doesn't go >7.2, I see naOh as a superior solution.

A highly concentrated sodium bicarb has a pH of ~8.5 vs naOH which has a pH of ~15. This means you cannot dump a crap load of naOh inside at once as it causes the pH to spike and destroy your glut.

If you are fine with a lower concentration then sodium bicarb would probably work, but what some of us are aiming for is 700+ mg/ml instead of 200mg/ml of glut.

To add on, if you want an even more hands-off approach and do not mind a lower concentration of glut, you can consider PBS buffers. You can get it highly concentrated (powder or liquid) at a pH of 7-7.2 and not worry about it ever going above 7.2.

You will need alot more PBS since it's a much weaker base (but it's cheap) but at least you do not have to worry about sodium bicarb or naOh raising the pH above 7.2.

 

[Mac81]

That's pretty close to what I found too photon.
Phosphate Buffers (PBS or Potassium Phosphate
Tris-HCl Buffers
MES Buffer
HCI
NAOH

Obviously L-Carnitine can be used as a buffer, And I'm sure 10 other things....I'm not going to list the exact steps for each way, because anybody can look it up...There's many ways of doing it. Each one of them has their pros and cons, There is no completely perfect way of doing it, You're always going to get some sort of oxidization at some point over a period of time,how much it takes place is not clearly stated. If you want the best possible result ,you should make the least amount possible at a time and try to avoid long-term storage. I would imagine that is why it's generally shipped as a dry powder in a vial, and reconstituted as needed

 

[JustJoe]

I mostly agree with u guys but using nAOH sets the ph once while using a buffer keeps it stable like a thermostat. NAOH is easy and quick. BUT nahco3 will get your way more mg/ml

A buffer maintains a consistent pH even when the environment changes. This helps keep glutathione in its active, reduced form

You just start by making 8% bicarbonate then add the GSH

Glutathione is more soluble in bicarb than in NaOH While glutathione is soluble in water, it is more readily dissolved in sodium bicarbonate buffer at higher concentrations, according to a study on ScienceDirect. Specifically, the study observed that GSH was insoluble in water at concentrations above 50 mg/mL but was dissolved at much higher concentrations using sodium bicarbonate buffer, according to ScienceDirect.

 

[Photon]

That's pretty close to what I found too photon.
Phosphate Buffers (PBS or Potassium Phosphate
Tris-HCl Buffers
MES Buffer
HCI
NAOH

Not all buffers you stated should be injected, some are not buffers as well.

A buffer maintains a consistent pH even when the environment changes. This helps keep glutathione in its active, reduced form

That depends on whats in the buffer, and how concentrated it is. A buffer of 7 is not going to remain at 7 long when you add something highly acidic or alkaline. We are talking about 700mg/ml+..not 50mg/ml+ or 200mg/ml+

 

[Mac81]

I mostly agree with u guys but using nAOH sets the ph once while using a buffer keeps it stable like a thermostat. NAOH is easy and quick. BUT nahco3 will get your way more mg/ml

A buffer maintains a consistent pH even when the environment changes. This helps keep glutathione in its active, reduced form

You just start by making 8% bicarbonate then add the GSH

Glutathione is more soluble in bicarb than in NaOH While glutathione is soluble in water, it is more readily dissolved in sodium bicarbonate buffer at higher concentrations, according to a study on ScienceDirect. Specifically, the study observed that GSH was insoluble in water at concentrations above 50 mg/mL but was dissolved at much higher concentrations using sodium bicarbonate buffer, according to ScienceDirect.

I think you need to get yourself some glutathione and try it for yourself.. That's the only way you're going to understand what we're saying. You're never going to even come close to the same concentrations using sodium bicarbonate...photon explained it best so I'm not going to repeat what he already said, but he was spot on with his explanation.. The same thing takes place when you use L-Carnitine,It works fantastic as a buffer and as a way to adjust the pH, but you can only achieve low concentrations, once you pass the 200 mg mark, you need more L-Carnitine, And it takes 600 mg of L-Carnitine to dissolve that 200, adding more just makes you lose your concentration, And baking soda is going to do the same thing because it's simply isn't strong enough, It works fantastic like you're saying but only in low concentrations... The only way you're going to understand is to do it yourself.. you can read all the articles you want online, but until you actually sit down and try to mix some up, You're not going to grasp what we're trying to explain to you.. The baking soda is buffering it, but it is also raising the pH which allows you to dissolve more into the solution, baking soda is 2 weak of a base to achieve the concentrations we are trying to get

 

[Mac81]

You can still add baking soda to it If you want to protect it and make it not oxidize, but you're still going to need NAOH added to it as well... I already tried adding baking soda to it before .. It barely even budged the pH.. It was actually worse than the 10% NAOH .. I tried adding more and more, when I realized how much it was going to actually take to have an effect on the pH I just gave up because it was doing worse than the L-Carnitine did, once you attempt it a couple times you'll understand what we are telling you, I don't know what they are calling high concentrations with baking soda, but it's not the kind of concentrations we are shooting for. You need something extremely potent to get the pH to go up so that it will continue to take more and more glutathione, baking soda simply will never do that, you'll end up with more baking soda in the solution than glutathione, before you hit any high concentration