Injectable Glutathione Recipe

[Sampei]

You are off the mark again. The calibration weight is 10g. The further you are from the calibration weight, the less accurate the scale is.

I use plastic try’s to weigh on. Using a beaker only sends you further from the calibration weight.



Yes, it is evaporating even at room temperature. The rate at which it evaporates increases with temperature.


Clearly the missing, let’s say 3 mL, is not all BA. I just don’t know how much of it would be the BA.

Probably just going to put in .5%.

BA hasn't evaporated lol

I leave all my oil brew at 65c for well past 30 minutes to mix and not once I have been shorted any amount.

There was one guy here that messed up and used BA instead of BB and tried to make it evaporate from the brew to fix it... I believe it took a few days to even lose a super small %.

You are being a sensitive little kid, he hasn't attack you in anyway.

 

[LGOP528]

I leave all my oil brew at 65c for well past 30 minutes to mix and not once I have been shorted any amount.

Reading more into this last night, I found that being in oil solution slows the rate of evaporation down compared to an aqueous solution.

BA hasn't evaporated lol

Am I overthinking this, probably but that is part of the fun.

You are being a sensitive little kid, he hasn't attack you in anyway.

My wife always calls me sensitive but I don’t believe her.

 

[burkeen]

I really struggled to get it right and I wanna save people looking for the recipe some time so they don't have to go through all posts.

This is the recipe for 30 ml at 200 mg/ml:

1) Weigh out 6 grams of Reduced Glutathione powder and pour it in your mixing beaker.
2) Add around 20 ml of Bacteriostatic water and start mixing (I prefer magnetic stirrer).
3) After a couple of minutes a milky cloudy solution will form. To get it to completely dissolve you will need to increase te pH of the solution.
4) Add NaOH pellets or NaOH solution to your mix. Add it very slowly, ml by ml or a couple of mg by couple of mg. If you get the pH above 6.5 it will oxidize instantly turning red and ruining your product. Having a pH meter is a life saver. The solution will start clearing up before 4.0 but I like to keep my brew between 4.5 and 4.9.
5) Once you've achieved your desierd pH, the solution should be crystal clear.
6) Add bacteriostatic water up to 30 ml and mix for another 5-6 minutes.
6) You can now filter your solution into a vials through 0.22 micron filter (I prefer Nylon66).
7) Once you've done you should store your Glutathione. If you are going to use it in the next 1-2 months you can store it in the fridge under 5 degrees C. If you are not going to use it soon then you should put it in the freezer and unfreeze it when you are ready to use it.

Possible to use sodium bicarbonate instead as Ph buffer?
Sorry if this is already asked and answer

 

[Photon]

Tried brewing a pure glut solution today at 600+ mg/ml.

What a headache.

How is it that nobody has mentioned that sodium hydroxide produces alot of heat when it dissolves? The last thing you need it the solution being at 55+ celcius each time you dissolve a little sodium hydroxide in it...and you need to dissolve alot of it which drives it even higher -- anyone dumping this in is probably destroying alot of glut in the process.

I tried making a concentrated sodium hydroxide solution with a ph of 14 like it should be with the intention of adding it cold to the mix, but the pallets refuse to dissolve once the ph hits 10.

Failed attempt i guess, will figure it out next week.

 

[MadaFacor]

Thank you! Do you notice anything taking that? What do you take it for if you don‘t mind me asking.

Read or Listen to the Glutathione Revolution. It's the most effective antioxidant and great for liver health among many other things.

 

[Photon]

Tried brewing a pure glut solution today at 600+ mg/ml.

What a headache.

How is it that nobody has mentioned that sodium hydroxide produces alot of heat when it dissolves? The last thing you need it the solution being at 55+ celcius each time you dissolve a little sodium hydroxide in it...and you need to dissolve alot of it which drives it even higher -- anyone dumping this in is probably destroying alot of glut in the process.

I tried making a concentrated sodium hydroxide solution with a ph of 14 like it should be with the intention of adding it cold to the mix, but the pallets refuse to dissolve once the ph hits 10.

Failed attempt i guess, will figure it out next week.

I think i figured it out.
I was able to dissolve 66gNAC and 200gGlut in 300ml of solution total.
Sadly I overshot the ph at the end and it ended up too high and my whole solution turned red. It looks like the one on the leftmost, which means its pretty much useless, but it also means that all of it did dissolve in a total of 300ml, which makes it 220/660 (assuming 100% purity). It's really thick tho, i'm thinking 200/600 might make more sense, so q.s to 330ml.

Either way, will try again tomorrow.

For those attempting this, i would suggest that you not use pellets, it produces too much heat and it's too slow. What you want is a highly concentrated liquid sodium hydroxide solution.

Ideally it should end up like the rightmost.

 

[LGOP528]

I think i figured it out.
I was able to dissolve 66gNAC and 200gGlut in 300ml of solution total.
Sadly I overshot the ph at the end and it ended up too high and my whole solution turned red. It looks like the one on the leftmost, which means its pretty much useless, but it also means that all of it did dissolve in a total of 300ml, which makes it 220/660 (assuming 100% purity). It's really thick tho, i'm thinking 200/600 might make more sense, so q.s to 330ml.

Either way, will try again tomorrow.

For those attempting this, i would suggest that you not use pellets, it produces too much heat and it's too slow. What you want is a highly concentrated liquid sodium hydroxide solution.

Ideally it should end up like the rightmost.

View attachment 337662

This is what I use. It is small grains.

Amazon.com

I have a lab style PH meter that I got off of eBay. The probe stays in the whole time. It takes time for everything to react, even with the small grains, so I watch the PH stabilize before adding more.

 

[Photon]

This is what I use. It is small grains.

Amazon.com

I have a lab style PH meter that I got off of eBay. The probe stays in the whole time. It takes time for everything to react, even with the small grains, so I watch the PH stabilize before adding more.

Yea thats the one i got initially and do not recommend, produces too much heat when it dissolves. I got a liquid one instead and add it dropwise using a 10ml syringe.

 

[LGOP528]

Yea thats the one i got initially and do not recommend, produces too much heat when it dissolves. I got a liquid one instead and add it dropwise using a 10ml syringe.

Well you are removing the heat from
dissolution but you are not removing the heat from neutralization when you switch to a solution.

It looks like you are just adding everything way too fast. Assuming no heat loss and complete reaction, it takes 1.73g of NaOH to raise 30mL of an acidic solution from 25C to 60C. That combined with the fact that you are over shooting the PH to the point of oxidation tells me you are adding too much, too fast.

I add very small amounts and wait 10 minutes or more, this allows the PH to settle as well as time for the heat to dissipate. I will add a thermometer next time to confirm but this has not been a problem for me.

 

[Photon]

Well you are removing the heat from
dissolution but you are not removing the heat from neutralization when you switch to a solution.

It looks like you are just adding everything way too fast. Assuming no heat loss and complete reaction, it takes 1.73g of NaOH to raise 30mL of an acidic solution from 25C to 60C. That combined with the fact that you are over shooting the PH to the point of oxidation tells me you are adding too much, too fast.

I add very small amounts and wait 10 minutes or more, this allows the PH to settle as well as time for the heat to dissipate. I will add a thermometer next time to confirm but this has not been a problem for me.

Yea that's too slow.
I'm doing 300ml at 800+ mg/ml (nac+glut). It'll take ages if i had to wait for it to dissolve etc..I overshot during the last bit as i got impatient, was aiming for ph 6.4 to use for storage.

Either way, trying again tomorrow, will use cold sodium hydroxide.

 

[Photon]

Yea that's too slow.
I'm doing 300ml at 800+ mg/ml (nac+glut). It'll take ages if i had to wait for it to dissolve etc..I overshot during the last bit as i got impatient, was aiming for ph 6.4 to use for storage.

Either way, trying again tomorrow, will use cold sodium hydroxide.

I did manage to brew it at 200/600 NAC/GLUT, however that crap is not usable. Its too thick, I'm struggling to even filter it.

I'm not sure how pharma does it, it's almost like the lyophilization process makes it easily dissolvable at high concentrations. With pharma, 700+mg/ml is not an issue. When brewing, i don't think going anywhere 600 is worth it. I ended up diluting it to 450/150, still thick..but 150mg injectable NAC is ~ 1.5g oral so it probablty doesnt make sense to inject lol. And injecting something with that smell daily..

For those using sodium hydroxide, make sure you either use PPE or have good ventilation. It's can be pretty bad irritant for asthmatics.

 

[Photon]

Has anyone seen their glut change color..when they add BA?
Kinda annoyed that mine turned red after i added 1% BA..
BA is acidic too..doesn't even make sense..
~650mg/ml..

 

[Deskoze38192]

I’m following going to be brewing this soon
Anyone know the benefits to adding NAC to this vs. oral pills? Does adding NAC make it harder to stay in solution and get proper PH?
I know the benefits of using NAC with glut
Was going to start with concentration of 200 mg/mL of just Glut
Anyone have experience at this lower concentration?

 

[Photon]

I’m following going to be brewing this soon
Anyone know the benefits to adding NAC to this vs. oral pills? Does adding NAC make it harder to stay in solution and get proper PH?
I know the benefits of using NAC with glut
Was going to start with concentration of 200 mg/mL of just Glut
Anyone have experience at this lower concentration?

You can just do sodium chloride (saline) + glut for 200mg/ml.
Probably wont even need sodium hydroxide.
Should be relatively easy to brew.

NAC has poor oral bioavailability (~10%), Only a small amount of NAC converts to glut. it is also more acidic than glut and needs to stay at a lower pH to not degrade. Lower pH injections sting more. Both are however acidic and you should have no issues mixing both.

NAC can be brewed at much higher concentrations, similar to L-Carnitine.

 

[LGOP528]

I’m following going to be brewing this soon
Anyone know the benefits to adding NAC to this vs. oral pills? Does adding NAC make it harder to stay in solution and get proper PH?
I know the benefits of using NAC with glut
Was going to start with concentration of 200 mg/mL of just Glut
Anyone have experience at this lower concentration?

I do not think adding NAC is necessary. NAC is converted to cysteine in the body, cysteine is the rate-limiting amino acid in glutathione synthesis. So adding a ton of NAC does not make sense to me seeing as how you are already injecting glutathione. NAC has other functions so I still supplement it at 500 mg a day orally. You can overdo antioxidants.

 

[JustJoe]

Has anyone seen their glut change color..when they add BA?
Kinda annoyed that mine turned red after i added 1% BA..
BA is acidic too..doesn't even make sense..
~650mg/ml..

View attachment 338508
View attachment 338509

It’s a tricky bitch. Pink means you oxidized some of the GSH, and honestly it’s ruined.

I crying over a beaker right now that was supposed to be 30ml: obviously it grew. Was adding 1M NaOH and the ph was raising .1 with every 3ml, I got frustrated and started added pellets.

I added the NaOH too fast, it got too hot, oxidized and turned pink at 6.5 pH. Tried adding SMB and EDTA to salvage, it went from red to pink but no dice.

It’s Trash

Lesson: add >10M NaOH in solution SLOWLY
View attachment IMG_1814.mov

 

[Photon]

It’s a tricky bitch. Pink means you oxidized some of the GSH, and honestly it’s ruined.

I crying over a beaker right now that was supposed to be 30ml obviously it grew.

I added NaOH too fast, it got too hot, turned pink. Tried adding SMB and EDTA to salvage but no dice.

Trash
View attachment 340734

I'm still injecting it daily 1ml lol. i brewed 150ml at like 700+mg/ml, got 5 amber vials of it in my fridge now. I realize that if you put it into a syringe, it actually is clear white. It's probably a tiny bit oxidised vs completely destroyed but looks bad because you are looking at a huge vial of it.

If you see this chart.
All of this shows varying levels of oxidation at small amounts of liquid. With that amount of liquid shown in the picture, my brew was actually completely clear. It's just thick like candy and needs a 29g insulin pin to pin.

You can salvage it quickly by adding NAC, try that next time.
It drops the pH extremely quick and stops oxidation immediately.

Pellets is not a good idea, i use a 50% naOH solution and add it dropwise using a syringe.

 

[JustJoe]

I may have salvaged mine. I added 10mg more of EDTA which binds to heavy metals a chelates them, . My solution is almost clear now.
If the EDTA addition pulled most of the pink out, then my color shift was from trace metal contamination rather than bulk GSH oxidation. Probably in the NaOH pellets.

Not sure if I feel comfortable trying this, but I may. It worked out 294mg/ml

Also, not all the EDTA is dissolved yet, which usually takes raising the temp <sweating and smiling nervously emoji>

 

[Photon]

I may have salvaged mine. I added 10mg more of EDTA which binds to heavy metals a chelates them, . My solution is almost clear now.
If the EDTA addition pulled most of the pink out, then my color shift was from trace metal contamination rather than bulk GSH oxidation. Probably in the NaOH pellets.

Not sure if I feel comfortable trying this, but I may. It worked out 294mg/ml

What displacement are u using? ~.7?
Why not just do a 700mg/ml and pin less liquid.
At 800mg/ml i could not push through a .22 filter

Can that EDTA be filtered out?
Isn't that a chelation agent?

 

[JustJoe]

Also, just a plug for these beakers I bought: Heavy duty EISCO brand. They are 5.3mm thick and the bottom is COMPLETELY flat so stirring is completely silent. Also they won’t shatter with heat or if you bump them in the sink.

The ULAB brand on Amazon are only 1.5mm thick, thinner than my freakin champagne glasses.

ULAB:



EISCO: