Lypholized HGH stored in freezer?

[ThePepBrother]

I’ve read conflicting information regarding storage of lyophilized HGH. Some say refrigerator, some say freezer, some say cool dark place. What is the best storage option?

 

[Ghoul]

 

[ThePepBrother]

Cool idea bro, and I’m sure this would be the BEST option, but I’m definitely not looking to buy a lab storage freezer. I’m guess I should have clarified that I’m looking more for a “home-based solution” that I could readily buy without breaking me.

 

[Ghoul]

Cool idea bro, and I’m sure this would be the BEST option, but I’m definitely not looking to buy a lab storage freezer. I’m guess I should have clarified that I’m looking more for a “home-based solution” that I could readily buy without breaking me.

Unless you have to manually defrost your freezer from time to time, store them in a lightproof container, all the way in the back of the lowest point of your refrigerator, that's not a moving drawer.

 

[ThePepBrother]

Yeah, my freezer for my peptides is a manual defrost freezer, which I keep at -7° Fahrenheit. It does a good job at keeping them at that temp. My concern is that I’ve read storing lyophilized HGH in a freezer isn’t recommended because it degrades in the freezer.

 

[UncleBuns]

I’ve read conflicting information regarding storage of lyophilized HGH. Some say refrigerator, some say freezer, some say cool dark place. What is the best storage option?

Any of those choices is fine. Depends on how long you want to store it for. If you want to store it for like 5 years or more I'd go freezer. Personally I put it in the freezer because why not. It's not going to hurt it and will greatly slow, whatever minimal degradation would happen in a closet. There are some threads here talking about minor variability in fridge or freezer temp affecting the product and I think all that is nonsense. At the end of the day just keep it from getting hot and definitely keep it out of sunlight and you're good to go.

 

[ThePepBrother]

Any of those choices is fine. Depends on how long you want to store it for. If you want to store it for like 5 years or more I'd go freezer. Personally I put it in the freezer because why not. It's not going to hurt it and will greatly slow, whatever minimal degradation would happen in a closet. There are some threads here talking about minor variability in fridge or freezer temp affecting the product and I think all that is nonsense. At the end of the day just keep it from getting hot and definitely keep it out of sunlight and you're good to go.

I don’t plan on long-term storage - maybe a year at the most. Just want to have enough on hand to get me through a few cycles and not have issues with storage degradation.

My thought process for the freezer storage is that if keeping it frozen was bad for it, then the lyophilization process itself would be bad for it. Since I know that’s not the case, I’m good with keeping it frozen at -7° or more until I’m ready to reconstitute and use it.

 

[Ghoul]

I don’t plan on long-term storage - maybe a year at the most. Just want to have enough on hand to get me through a few cycles and not have issues with storage degradation.

My thought process for the freezer storage is that if keeping it frozen was bad for it, then the lyophilization process itself would be bad for it. Since I know that’s not the case, I’m good with keeping it frozen at -7° or more until I’m ready to reconstitute and use it.

The reason for the warning against repeated freeze thaw cycles is the damage from ice crystals forming from the residual moisture mechanically breaking peptide chains.

UGL peptides have been found to have significantly more residual moisture than those produced in professional labs. I've seen it as high as 2%. It's likely even higher in others.

Your freezer isn't a rock solid -7f. It almost certainly varies by 5 degrees or more. Your compressor isn't turning on a -6f and off at -8f. It would quickly burn up under those conditions.

So the question is, what temperature does the water content of the lyophilized protein freeze?

 

[ThePepBrother]

The reason for the warning against repeated freeze thaw cycles is the damage from ice crystals forming from the residual moisture mechanically breaking peptide chains.

UGL peptides have been found to have significantly more residual moisture than those produced in professional labs. I've seen it as high as 2%. It's likely even higher in others.

Your freezer isn't a rock solid -7f. It almost certainly varies by 5 degrees or more. Your compressor isn't turning on a -6f and off at -8f. It would quickly burn up under those conditions.

So the question is, what temperature does the water content of the lyophilized protein freeze?

Good point! Would it be safe to say that as long as the temperature doesn’t go above the freezing point of water, then there should be no concern? At ~-7° Fahrenheit, I don’t think there would be a problem.

 

[Ghoul]

Good point! Would it be safe to say that as long as the temperature doesn’t go above the freezing point of water, then there should be no concern?

As long as it's not going below the freezing point of water, there's no risk of mechanical stress damage to a peptide. That only leaves chemical (and possibly bacterial) degradation. To slow those to a minimum the colder the better.

 

[sushak]

If you must use a regular kitchen freezer, at least store vials in thermos flask in the freezer to smooth out the temp fluctuations and prevent thawing during the auto defrost cycles

 

[ThePepBrother]

Agreed if you have a freezer with auto-defrost. Even if you don’t have auto-defrost, the thermos flask storage keeps them “safe” in the event of a power outage. That is what I do, except I have a separate freezer for my peptides that is manual defrost.

 

[Ghoul]

Good point! Would it be safe to say that as long as the temperature doesn’t go above the freezing point of water, then there should be no concern? At ~-7° Fahrenheit, I don’t think there would be a problem.

Water in contact with any impurity will have a freezing point lower than pure water, 32f.

Sodium chloride changes the freezing point to, for instance, -6f. So a freezer cycling between -4f and -9f will be freezing and thawing the peptide multiple times a day.

Of course we have no idea what the freezing point is of the residual water in a particular peptide is, other than it's definitely lower than 32f and above about -60f. That's why a -80c (-112f) freezer is ideal. Even with a swing temp, water will always remain frozen regardless of what the modified freeze point is.

It's not the dry, lypholized peptide that's freezing. That's not why you don't want repeated freeze/thaw cycles. It's the residual moisture from an imperfect freeze drying process.

 

[Ateam2023]

i store my hgh/peptides in an airtight container inside the refrigerator,,,,, And i just buy a 7-8 months worth of hgh and 3-4 months worth of peptides, i also reconstitute with room temperature bac water into cold hgh/peptide with no problems, for over year and half and i use Hospira bac water ,,,,,,

 

[Ghoul]

Let me save everyone some time and preempt this inevitable comment:

"But why would there be any sodium chloride in a peptide! More theoretical nonsense from Ghoul"

Sodium chloride, mannitol, dextrose, and glycerin are the four most common tonicifying excipients listed in pharma peptide therapeutic product inserts.

And this one:

"But professional peptide production labs advise freezing peptides at -20c!"

Those are isolated peptides, free from the excipients found in the 'ready to use' peptide formulations we get.