Media Bottle to Vials

[Oroku_Saki]

What the fuck? You’re nuts ..

Yes, literally. I regret that I lost my composure a few posts earlier. He is one of those simple people who get satisfaction from that. Probably because outside of the internet they are only very small people, possibly failed in life so far.
Anyway, he's the first user to end up on my ignore list.

 

[Cashton]

Yes, literally. I regret that I lost my composure a few posts earlier. He is one of those simple people who get satisfaction from that. Probably because outside of the internet they are only very small people, possibly failed in life so far.
Anyway, he's the first user to end up on my ignore list.

I’ll follow you in that, first user on my ignore list.

 

[klokke23]

Yes, literally. I regret that I lost my composure a few posts earlier. He is one of those simple people who get satisfaction from that. Probably because outside of the internet they are only very small people, possibly failed in life so far.
Anyway, he's the first user to end up on my ignore list.

Funny. I always pictured people who frequent a board EVERY day to be failed people. Get some real friends or something. Wtf are you doing a Friday night sitting on a board for the 2000th day in a row

 

[Oroku_Saki]

Yes, smartphones are a new thing.

I will sit outside by the pool in my garden in my townhouse in a major city and let the tears I shed over my failed existence dry in the sun.

Thank you for opening my eyes little man.

 

[xmilar]

You can use what ever size you want you just need to make sure the neck size is 33mm not 45mm, or it will cost alot more.

https://www.medical-and-lab-supplies.com/lab-glassware/media-bottles/caps/33mm-caps-with-attached-silicone-septas.html

How do you sterilize these caps and septas?

 

[Oroku_Saki]

How do you sterilize these caps and septas?

It is literally written in the description.
-Autoclavable

 

[klokke23]

Backfilling syringes is dangerous because dust is toxic. Alert the hospitals and WHO. Their practice of prefilling syringes for later use is dangerous! Mold in the air, poison under our skin

 

[CHRIS VERDI]

Backfilling syringes is dangerous because dust is toxic. Alert the hospitals and WHO. Their practice of prefilling syringes for later use is dangerous! Mold in the air, poison under our skin

Are you retarded?

 

[klokke23]

Are you retarded?

If you genuinely believe dust is any hazard to yoh at all, you are the one who's retarded. You inject air into the vial, and you get air in your shit when backfilling a syringe.

 

[CHRIS VERDI]

If you genuinely believe dust is any hazard to yoh at all, you are the one who's retarded. You inject air into the vial, and you get air in your shit when backfilling a syringe.

When did I say this?

 

[CHRIS VERDI]

When did I say this?

If you genuinely believe dust is any hazard to yoh at all, you are the one who's retarded. You inject air into the vial, and you get air in your shit when backfilling a syringe.

And yes it is a possibility, people have died during surgery from a high air pollution of bacteria. Am I concerned about that no not really but it is absolutely possible.

 

[klokke23]

And yes it is a possibility, people have died during surgery from a high air pollution of bacteria. Am I concerned about that no not really but it is absolutely possible.

Now you're being retarded. It's a possibility someone died from too much ketchup as well. So fucking what

 

[CHRIS VERDI]

Now you're being retarded. It's a possibility someone died from too much ketchup as well. So fucking what

Now your being silly. No one has ever died from too much ketchup. Even off brand.

 

[CHRIS VERDI]

Now you're being retarded. It's a possibility someone died from too much ketchup as well. So fucking what

Oh can you post your nipples and dick please.

 

[Plate Smacker]

I just backfill a 60ml syringe.
Directly into sealed sterile vials.
Air purifying in room for 6 hours.
2x4 across 2 stands, at stomach level.
2x4 has hole drilled that the 60ml syringe fits through.
Wipe down 2x4.
Wipe off tops of sealed sterile vials.
Place 27G into first sterile vial for venting.
Place 60ml syringe through hole in 2x4.
Place 20G needle on end of syringe.
Remove plunger (this stucks air through syringe of course). Don't let rubber of plunger touch anything.
Immediately place 20G into sealed sterile vented vial.
Open media bottle and pour directly into 60ml syringe.
Immediately recap media bottle.
Then Immediately put plunger back into 60ml syringe and there will be small air pocket on top of gear in syringe.
The gear will Immediately start flowing into syringe.
When gear gets to almost fully in vial, pull plunger back slightly to relive pressure. Otherwise when you remove vial gear will flow from 20G and waste.
Remove vent 27G first and vent next vial, have new vented vial in hand.
Remove full vial and Immediately Place new vented vial on end of 20G.
Restart pressure and fill new vial.

Once syringe empty, don't push the air pocket into final vial.
If the last vial was not full, leave vent in and remove vial from 20G.
Quickly pull back plunger, and Place vial back on 20G. You don't want to remove plunger while 20G is in vial.
Refill syringe and repeat.

There will be some drips once syringe is empty and you remove vial to remove plunger. Then Quickly placed vial back on end of 20G before refilling syringe.

I would make video,, but to many identifying tattoo on arms.

I've never had anything floating in my vial. I've never had an infection.