MESO "Specialized" Testing Group Fund

[B Ware]

Jano’s team notified me this morning they received my NPP sample. At which point I added gcms testing. No reimbursement required. I was already testing the sample and I’m happy to contribute.

 

[Photon]

Jano’s team notified me this morning they received my NPP sample. At which point I added gcms testing. No reimbursement required. I was already testing the sample and I’m happy to contribute.

Wait till after you get purity results.

 

[B Ware]

Wait till after you get purity results.

I explained that and was still asked for the funds. If it comes back not to be NPP I’ll have a credit, which is fine by me.

 

[Sampei]

Jano’s team notified me this morning they received my NPP sample. At which point I added gcms testing. No reimbursement required. I was already testing the sample and I’m happy to contribute.

Thanks mate appreciated very much

 

[Sampei]

Janoshik Analytical

first GCMS from TNE just came in

 

[sionnach]

4,6-Androstadien-17.beta.-ol-3-one

Interesting

 

[dinfar1337]

Interesting

ye it should be only testosterone in there.

the tne is either mixed up with rest 13-14% of bad stuff or its from poor quality synthesis like my guess which is giving color.

these are all testosterone byproducts.

no harsh solvents or interesting stuff left in there to be found

 

[Sampei]

Interesting

I have no clue how to read that GCMS, hopefully someone will chime in and we will have a better idea when we compare those other different samples

 

[dinfar1337]

I have no clue how to read that GCMS, hopefully someone will chime in and we will have a better idea when we compare those other different samples

Post in thread 'Δ6-testosterone enanthate'

Aug 6, 2025

Very interesting. So your conclusion is this molecule will be converted to testosterone via cytochrome enzymes.

Yeah, I never made any claims on the PIP. It is just this work by Jano intersected with the stuff we found in the raw on one of the projects. Thanks for the info.

yes, i also looked at janos message and he didnt know what it was(or said he didnt know if it caused pip). it shouldn't do that tho, u can read the description here but its very hard to find anything on this shit since its just byproduct by bad quality testosterone synthesis probably...

• dinfar1337

• 

that 4,6-Androstadien-17.beta.-ol-3-one 10% me and @readalot cleared up 1-2 months ago

rest is uncertain auto ids probably closely linked to testosterone aswell

 

[dinfar1337]

normal testosterone = Δ4-androsten-17β-ol-3-one

delta6 testosterone by poor manufacturing = Δ4,6-androstadien-17β-ol-3-one

 

[sionnach]

normal testosterone = Δ4-androsten-17β-ol-3-one

delta6 testosterone by poor manufacturing = Δ4,6-androstadien-17β-ol-3-one

Yeah if I'm reading correctly, that particular component in the results just didn't get fully converted to test. And 10% is a lot.

 

[dinfar1337]

Yeah if I'm reading correctly, that particular component in the results just didn't get fully converted to test. And 10% is a lot.

that is testosterone

Δ4,6 read carefully.

in normal testosterone we only have double bond at c4-c5

in delta 6 we have double bound at c6-c7 also.

its quite literally a byproduct of testosterone from oxidation(poor storage of raws or poor synthesis)

and as you observe 10% is quite alot even tho the % are not accurate im sure it isnt that far off

it should only be testosterone in there so u can just 100-86 and we're at 14% brother

 

[Sera]

This is crazy. Jano brought out the long-run GCMS for us ? Very curious. You see how close the 4,6-ANDROSTADIEN-17-BETA-OL-3-ONE is to the testosterone, usually it probably wouldn’t be detected by HPLC.

 

[dinfar1337]

This is crazy. Jano brought out the long-run GCMS for us ? Very curious. You see how close the 4,6-ANDROSTADIEN-17-BETA-OL-3-ONE is to the testosterone, usually it probably wouldn’t be detected by HPLC.

However not the usual kind, but delta 6 testosterone enanthate, which is listed, at least in European Pharmacopoeia, as "Impurity F" with maximum permissible limit of 0.3%.

looking it up its just an aas, you can be right in saying its a impurity from testosterone synthesis in the sense its not what you wanted to get but it acts completely as testosterone besides the stuff i will talk about down below.

but it will be metabolized through the liver into testosterone, just as normal test e would be metabolized into testosterone through the p450 enzyme. its basically just testosterone with higher binding affinity to the ar. metabolism rates might slightly differ because its higher binding affinity to the androgen receptor

so all in all, its completely normal testosterone. i dont see how this is the thing causing pip

yes, i also looked at janos message and he didnt know what it was(or said he didnt know if it caused pip). it shouldn't do that tho, u can read the description here but its very hard to find anything on this shit since its just byproduct by bad quality testosterone synthesis probably.

https://www.biosynth.com/p/FT28049/2484-30-2-delta6-testosterone

Wow, thank you for this reference. So according to this there would be no need to include this "impurity" in the impurity spec since it converts to Test once in the body. Very cool. Really appreciate it.

@Sampei what tne sample was this? tne clean or the newer bad one?

 

[Sera]

Which first test sorry?

 

[dinfar1337]

Which first test sorry?

edited my message i dont know either

 

[dinfar1337]

here doing some spoon feeding now

@readalot for me being lazy and telling u to go google yourself 3 months ago is kicking in me in my own ass now

https://www.sciencedirect.com/science/article/abs/pii/S009095562508701X

 

[Sampei]

@Sampei what tne sample was this? tne clean or the newer bad one?

This is the newer "bad" one but I would call it bad, I injected it and it's smooth and nice xD

Tested 98% purity on HPLC

For my rudimentary understanding the GCMS looks quite ok

 

[dinfar1337]

This is the newer "bad" one but I would call it bad, I injected it and it's smooth and nice xD

i also cleared the air with readalot on the delta 6 testosterone dosent cause pip and is most likely just gonna be like normal testosterone.

the many peaks and delta 6 testosterone suggests some oxidiation which could have caused color i dont think these auto ids with lower % are wrong, could very well be but its all testosterone byproducts which none of are dangerous anyway.

just shows a lack in QC from the lab which produced/stored it. we can tell if it was storage issue if the other lab shows good then its most likely manufacturer issue.

i deleted my thoughts before because i didnt know which one of the samples it was but thats most likely what this whole testing was about and even with lacking test results for the clean ones you can say the testing is pretty much concluded.

enjoy the TNE im gonna go grab some if the raws are still for sale

 

[Sampei]

i also cleared the air with readalot on the delta 6 testosterone dosent cause pip and is most likely just gonna be like normal testosterone.

the many peaks and delta 6 testosterone suggests some oxidiation which could have caused color i dont think these auto ids with lower % are wrong, could very well be but its all testosterone byproducts which none of are dangerous anyway.

just shows a lack in QC from the lab which produced/stored it. we can tell if it was storage issue if the other lab shows good then its most likely manufacturer issue.

i deleted my thoughts before because i didnt know which one of the samples it was but thats most likely what this whole testing was about and even with lacking test results for the clean ones you can say the testing is pretty much concluded.

enjoy the TNE im gonna go grab some if the raws are still for sale

Will be nice to compare it with the other products