MESO "Specialized" Testing Group Fund

[dinfar1337]

Issue is that GCMS wont give you purity% to brew oils.

i know.

thats why we're seeking hplc - lcms as a middle ground.

because hplc-dad on its own is proving worthless on its own

 

[Photon]

You can use proper GCMS method to get quantitative purity. Mg of API by GCMS divided by mg of raw. API is volatile and derivatization makes it even tighter.

Concern is whether HPLC giving us accurate purity for these raws.

If we can get the mg, that's definitely possible.
I don't believe it's currently provided tho.

 

[dinfar1337]

You can use proper GCMS method to get quantitative purity. Mg of API by GCMS divided by mg of raw. API is volatile and derivatization makes it even tighter.

Concern is whether HPLC giving us accurate purity for these raws.

i would 100% rather choose gcms than hplc-dad if i had to pick only one for raws.

think i said this couple of days ago

 

[readalot]

I don't believe it's currently provided tho.

Correct, Jano's current GCMS setup is not validated quantitative method.

As I said, GCMS is light years ahead of HPLC-DAD in terms of chromatographic efficiency. Beautiful tight peaks. We are chasing shadows right now.

 

[readalot]

i would 100% rather choose gcms than hplc-dad if i had to pick only one for raws.

think i said this couple of days ago

We still need a raw purity. I don't know what it is on these raws.

 

[Photon]

Correct, Jano's current GCMS setup is not validated quantitative method.

As I said, GCMS is light years ahead of HPLC-DAD in terms of chromatographic efficiency. Beautiful tight peaks. We are chasing shadows right now.

I believe I've asked before about using GCMS to cal purity.
I mistakenly mentioned mtren but i believe it's superdrol that uses GCMS to calc mg/ml.

Post in thread 'Janoshik Analytical laboratory testing services'

Oct 3, 2025

I know that for certain oils, GCMS is required to get the mg/ml (e.g mtren).
If we request for raw purity % to be calculated via GCMS, is it possible and is the cost 170$?

Mtren is thermally labile, thus it's near impossible to get proper quantities with GCMS. We use normal HPLC for mtren.

We don't use GCMS for purity measurements.

Cheers

• janoshik

• 

 

[readalot]

I believe I've asked before about using GCMS to cal purity.
I mistakenly mentioned mtren but i believe it's superdrol that uses GCMS to calc mg/ml.

Post in thread 'Janoshik Analytical laboratory testing services'

Oct 3, 2025

I know that for certain oils, GCMS is required to get the mg/ml (e.g mtren).
If we request for raw purity % to be calculated via GCMS, is it possible and is the cost 170$?

Mtren is thermally labile, thus it's near impossible to get proper quantities with GCMS. We use normal HPLC for mtren.

We don't use GCMS for purity measurements.

Cheers

• janoshik

• 

Yes, derivatization a must for thermally labile shit.

Too much to ask for the UG. Anyway, good luck everyone.

Derivatization Methods in GC and GC/MS

The first part of this chapter presents the main objectives for performing derivatization of a sample to be analyzed by gas chromatography (GC) or gas chromatography/mass spectrometry (GC/MS). The derivatization is typically done to change the analyte properties for a better separation and also...

www.intechopen.com

 

[dinfar1337]

We still need a raw purity. I don't know what it is on these raws.

"QNMR to be sure, integrate every hydrogen, 500 bucks." - mr janoshik

"We are chasing shadows right now." - mr readalot

 

[dinfar1337]

anyways @Sampei is there more test results coming?

 

[readalot]

"QNMR to be sure, integrate every hydrogen, 500 bucks." - mr janoshik

"We are chasing shadows right now." - mr readalot

"Hey guys, here's 10k US, let's do this right." - mr dinfar1337

 

[dinfar1337]

"Hey guys, here's 10k, let's do this right." - mr dinfar1337

i have 10 bucks

"my brother dinfar, here is 9990 dont worry we paid for it together!" - mr readalot

 

[readalot]

i have 10 bucks

"my brother dinfar, here is 9990 dont worry we paid for it together!" - readalot

 

[Sampei]

anyways @Sampei is there more test results coming?

I think there is more NPP coming, from a member that would like to remain anonymous for his own security.
I have more raws incoming tho, so we can do more tests on other raws if it helps...

 

[dinfar1337]

I think there is more NPP coming, from a member that would like to remain anonymous for his own security.
I have more raws incoming tho, so we can do more tests on other raws if it helps...

i think its better to hold off on raw testing.

wait until the raw situation changes if it does. and when raws are flowing in more smoothly we will see if the labs can improve.

until now just hold onto the funds.

most people that can do 1+1=3 will be able to see the raw situation for now.

 

[readalot]

i think its better to hold off on raw testing.

wait until the raw situation changes if it does. and when raws are flowing in more smoothly we will see if the labs can improve.

until now just hold onto the funds.

most people that can do 1+1=3 will be able to see the raw situation for now.

Best outcome from all of this work is if we could be confident in quantitative purity measurements for raws.

Are we picking up the correct purity for common AAS raws with standard HPLC method today?

The GCMS data above speaks for itself. Well done getting more GCMS data for raws.

 

[Driada Britannica]

How can we contribute to the fund?

 

[dinfar1337]

Are we picking up the correct purity for common AAS raws with standard HPLC method today?

nope

Best outcome from all of this work is if we could be confident in quantitative purity measurements for raws.

im all in, start begging jano in his thread and i will join!

 

[Photon]

The oils are all brewed with the raw purity calculated by hplc, if we say that the raw purity is not calculated accurately, isn't that also saying the oil mgml is not accurate since they're supposedly matching?

 

[dinfar1337]

The oils are all brewed with the raw purity calculated by hplc, if we say that the raw purity is not calculated accurately, isn't that also saying the oil mgml is not accurate since they're supposedly matching?

completely right!

hplc in mgml in oils and raws cant differentiate

in this example delta 6 test will show up as pure testosterone in hplc brewed im 99% sure off. just as it did in hplc raw testing

if it cant differentiate in raws it will never differentiate in oils

 

[readalot]

completely right!

hplc in mgml in oils and raws cant differentiate

in this example delta 6 test will show up as pure testosterone in hplc brewed im 99% sure off. just as it did in hplc raw testing

if it cant differentiate in raws it will never differentiate in oils

Go back to Project 3 a while back which was brought up again here recently.

Not knocking Jano at all, but completely unclear to me if the HPLC method is routinely registering false positives. Hence, any actual (1) raw purity or (2) mg/ml could be less than what is reported.

The GCMS and HPLC results on the C6-C7 -ene contamination of TC/TE raw samples demonstrated the situation clearly.