More Testing- Bacteria Culture

[Candyskull]

I'm glad to see so much testing of gear to ensure that it is what it says it is.

Now I'd like to address the other issue I'm seeing here. Is my gear dirty? Find out the way a lab would!!!

http://www.sciencecompany.com/Bacteria-Growing-Experiments-in-Petri-Dishes-W54C659.aspx

This is exceedingly cheap and easy. I've mentioned it to a couple other guys here, but no one seems to have done it yet. If you'd like to know if your gear is clean, just culture it. That will answer all questions about its sterility in short order.

You guys all rock. Keep meso at the forefront of juice head science

 

[boxer15]

Cool. Great post candyskull, I didn't even think that you could do this gear. Seems pretty simple. I might try this out in a couple of weeks.

 

[flenser]

You talked me into it. Just ordered some agar petri dishes. Might try swabbing the skin after preparing to pin just to see how f'ed up my procedure is.

 

[Candyskull]

You talked me into it. Just ordered some agar petri dishes. Might try swabbing the skin after preparing to pin just to see how f'ed up my procedure is.

Can't wait to hear the results! I especially liked the sleeps blood dishes since the organic material more closely matches ours. I'm going to test a few things in the spring when I get ready for that cycle. Right now I'm just going to do some skin swabs and things of that nature.

 

[BiochemicalAbomination14]

Can't wait to hear the results! I especially liked the sleeps blood dishes since the organic material more closely matches ours. I'm going to test a few things in the spring when I get ready for that cycle. Right now I'm just going to do some skin swabs and things of that nature.

Great idea by the way, good on you for pointing out this can be done.

What's even more fun than your skin? Test a doorknob, any random public doorknob and then swab the bottom of your shoe and compare! Or for even more shits and giggles, test money!

 

[strongsafety41]

I'm new to this forum as well as forums in general. I must say the science aspect (culture testing) and labmax testing really got my attention and made me want to be a part of this board! Flenser, thanks for the effort and diligence in your testing procedures.

 

[Hueghe]

These tests are a good way to keep these ugls somewhat honest, if they work that is.

 

[ebkallday]

Flenser, you're on it bro!! Keep us updated!!!!

 

[flenser]

Ha, of course I'm not the most hygienic person in the world. Finding something of mine that DOESN'T have a good bit of bacteria is pretty unlikely.

 

[stomps]

I have a pretty good idea how to do something similar to this that would help insure that if any contamination grew it would be from the product and not any air borne contamination.

I have to go to work till late but when I get home ill post my idea and maybe Dr. Jim could tell us his thoughts on it.

 

[stomps]

Ok so this might be a bit lengthy but I would like to hear your guys opinions on this ok here we go.

Supplies needed

Pressure cooker
Agar mix
Canning jar with self sealing lid( the ones with the disk and ring)
2 sterile whatman syringe filters
1 vial stopper
High temp silicone ( the red atv kind)
Sterile syringes

Step 1
Take the canning jar lid just the disk part and drill one hole for the stopper and use the silicone to seal it water tight and let set over night. Do the same for the whatman filters.
So basically you have a lid with a stopper and two whatman filters installed in it.

Step 2
Make your agar and pour about 1/4 inch to a 1/2 inch in the bottom of the jar.

Step 3
Assemble the jar lid and all so now you have a canning jar with agar and a lid with an injection port and whatman filters for sterile air exchange.

Step 4
Pressure cook the jar with agar and modified lid at 15 psi for 60 to 90 min.
Be sure to tighten the lid then give it a slight turn loose.
The jar will seal while pressure cooking it. Also wrap aluminum foil on the top so water doesn't drip on/in the whatman filters

Step 5
After the allotted time of pressure cooking it let it cool completely before opening the pressure cooker. Upon opening tighten the lids on the jar tight.
Let the jar set over night.

Step 6
After letting it sit over night the agar should be solidified by now.
Using the injection port/stopper inject some of the gear you want to test and tip the jar so it gets spread around the agar. Then wait and see.

Like I said I would like everyone's opinion.

 

[stomps]

I think my procedure above would provide a sealed sterile container so anything that grows would be either from the needle highly doubtful if you used sterile syringe and needle or the gear.

 

[fit2beking]

Pretty well thought out stomps! I'm curious to see the outcome.

 

[stomps]

Pretty well thought out stomps! I'm curious to see the outcome.

Thanks I will probably try this when I get the chance.

 

[Candyskull]

Ok so this might be a bit lengthy but I would like to hear your guys opinions on this ok here we go.

Supplies needed

Pressure cooker
Agar mix
Canning jar with self sealing lid( the ones with the disk and ring)
2 sterile whatman syringe filters
1 vial stopper
High temp silicone ( the red atv kind)
Sterile syringes

Step 1
Take the canning jar lid just the disk part and drill one hole for the stopper and use the silicone to seal it water tight and let set over night. Do the same for the whatman filters.
So basically you have a lid with a stopper and two whatman filters installed in it.

Step 2
Make your agar and pour about 1/4 inch to a 1/2 inch in the bottom of the jar.

Step 3
Assemble the jar lid and all so now you have a canning jar with agar and a lid with an injection port and whatman filters for sterile air exchange.

Step 4
Pressure cook the jar with agar and modified lid at 15 psi for 60 to 90 min.
Be sure to tighten the lid then give it a slight turn loose.
The jar will seal while pressure cooking it. Also wrap aluminum foil on the top so water doesn't drip on/in the whatman filters

Step 5
After the allotted time of pressure cooking it let it cool completely before opening the pressure cooker. Upon opening tighten the lids on the jar tight.
Let the jar set over night.

Step 6
After letting it sit over night the agar should be solidified by now.
Using the injection port/stopper inject some of the gear you want to test and tip the jar so it gets spread around the agar. Then wait and see.

Like I said I would like everyone's opinion.

I like it man. The only other thing I would do is alcohol wash the jar first. Other than that, I think it's perfect.

 

[stomps]

I like it man. The only other thing I would do is alcohol wash the jar first. Other than that, I think it's perfect.

Good addition sir
Thanks for your input

 

[stomps]

Anyone else have any input

 

[freak]

You could try the google scholar search engine. That specialized search engine produces a lot of great studies and protocols on research.

You might could find some techniques with agar, for growing bacteria aerobically.