clearheaded
Member
filtering doesn't clean vials...fyi
MESO-Rx
Anabolic Steroids
My Sterilization Process
- Thread starter Qualityoflife86
- Start date
clearheaded
Member
pressure cooker is the sterilizing step. home ovens are not sterile at any temp as constantly fresh dirty air is entering.
unclebob
Member
You ever heard of tinfoil?
And to counter your argument one could put a smaller oven inside a flow hood!
clearheaded
Member
foil does not seal and because of very high movement of air I promise shit gets in there, maybe not 100% of the time... but your right if actually seal it forsure would be helpful! but as always dry heat does not sterilize as well as steam because of transfer of heat, aswell as ovens temp drops unlike PC which is also part of why its sterile. there is a very good reason even in LABRATORIES SOP is steam as even expensive ovens just dont work as well. this is not your home oven with cheese splattered on the walls and evaporating grease circulating around. the second a bit of fresh air enters a vial you need to restart the timing. tinfoil is simply not a reliable seal in with moving air. can u get away with it? prob, just for 100$ u can get a PC and know with 100% its actually sterile and is the smartest safest way. to me its a big difference between prob ok and 100% ok. ESP when you dont have a flow hood and are not familiar in sterile technique as all adds up, every shortcut adds more risk and thats just reality. we can look to GMP manufacturing even, shit happens even with high standards and oversite SOPs etc and not in a bathtub. hear about those people dying and loosing eyes from eyedrops recently? shit happens. better tek=less chance it happens.
have you ever done any work in microbiology? poured any agar plates? may be surprised the shit that rains down, esp if happen to screw up % of bacteriostatic agents and store for several months.. there is a reason PCT places sell antibiotics and its not cause guys are getting infections from pharma grade sterile gear that has been handled properly.
do what you like, but for a harm reduction forum should not be promoting short cuts.
we have science and is repeatable that u sterilize in a home PC(why u do canning in a PC and not an oven), ever seen any CFU testing done on stuff "sterilized" in a home oven? so not only logic but EVIDENCE is why you need a PC if want 100% sterile.. otherwise its just bro science. literally 100$ or even less can provide 100% sterile..
have you ever done any work in microbiology? poured any agar plates? may be surprised the shit that rains down, esp if happen to screw up % of bacteriostatic agents and store for several months.. there is a reason PCT places sell antibiotics and its not cause guys are getting infections from pharma grade sterile gear that has been handled properly.
do what you like, but for a harm reduction forum should not be promoting short cuts.
we have science and is repeatable that u sterilize in a home PC(why u do canning in a PC and not an oven), ever seen any CFU testing done on stuff "sterilized" in a home oven? so not only logic but EVIDENCE is why you need a PC if want 100% sterile.. otherwise its just bro science. literally 100$ or even less can provide 100% sterile..
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So, is the part about using the oven useless? Can I just use the pressure cooker in the sterilization process?
clearheaded
Member
if want to be certain you have sterile things ya you want PC.. you can clean and dry and place in a jar or turkey bag to keep moisture off.
while yes possible in oven its not BEST to ensure clean and sterile.
clean, rinse, iso rinse, distilled water rinse, dry, PC... work infront of laminar flow hood or still air box (as at least prevent shit from raining into vials) with proper tech u can be 100% sterile in a SAB but of course laminar flow hood Is best... yes you inject air into vial but LESS germs in there is always best. I like leaving syringe cover on when pull air into syringe even
no evidence but makes sense, less you breath on the needle and bacteria/virus at least have to overcome a little gravity and tiny bit of clean air inside the cover at least, splitting hairs a bit though..
while yes possible in oven its not BEST to ensure clean and sterile.
clean, rinse, iso rinse, distilled water rinse, dry, PC... work infront of laminar flow hood or still air box (as at least prevent shit from raining into vials) with proper tech u can be 100% sterile in a SAB but of course laminar flow hood Is best... yes you inject air into vial but LESS germs in there is always best. I like leaving syringe cover on when pull air into syringe even
no evidence but makes sense, less you breath on the needle and bacteria/virus at least have to overcome a little gravity and tiny bit of clean air inside the cover at least, splitting hairs a bit though..
JollyRoger2x2
New Member
When you're doing the alcohol soak, make sure all surfaces of your tools are fully submerged. For bottles or vials, shaking them with alcohol inside works well, as you mentioned. Letting them soak for a bit before agitating helps break down any residue.
Sterilizing your tools properly is super important, and it’s great that you’re being thorough about it. After the initial cleaning steps, switching to medical grade gloves is definitely a good move.
Sterilizing your tools properly is super important, and it’s great that you’re being thorough about it. After the initial cleaning steps, switching to medical grade gloves is definitely a good move.
How do you dry the product before putting it into the PC, Oven dry? Isnt that just exposing it to what your arguing against.if want to be certain you have sterile things ya you want PC.. you can clean and dry and place in a jar or turkey bag to keep moisture off.
while yes possible in oven its not BEST to ensure clean and sterile.
clean, rinse, iso rinse, distilled water rinse, dry, PC... work infront of laminar flow hood or still air box (as at least prevent shit from raining into vials) with proper tech u can be 100% sterile in a SAB but of course laminar flow hood Is best... yes you inject air into vial but LESS germs in there is always best. I like leaving syringe cover on when pull air into syringe even
Drugsarefun
New Member
What's the reason for the alcohol?
Lab equipment prep does not include an alcohol bath, you have a soapy wash station followed by 2 distilled water rinse stations, alcohol is unnecessary.
Lab equipment prep does not include an alcohol bath, you have a soapy wash station followed by 2 distilled water rinse stations, alcohol is unnecessary.
Im no chemist nor expert, but from most my research alcohol sounds like a very common thing for sterilization of glass of the equipment. Soap, alcohol, 2 distilled rinses.
Very long winded response for saying you used to grow mushrooms
Drugsarefun
New Member
Common but not needed, if you look into any official lab equipment cleaning process you'll find no alcohol bath.
People pass on the miss information thinking it sterilises the equipment... It does not...
Also the purpose of cleaning your equipment is to remove any dust, soap scum, residue oils and water.
Sterilising is done in an autoclave or pressure cooker, oil is sterilised by passing through a 0.2um filter
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