Hey everyone, I'm literally about to have a bash at a home brew for the first time until I came a across a sudden issue yesterday. The whatman syringe filters have arrived all lose in one bag. The seller now tells me they were made in a clean environment but are not sterile. Is there a know way to sterilise these? Or should I just chuck them and order new ones which I know are actually sterile? Cheers
MESO-Rx
Anabolic Steroids
Non sterile .22 filter
- Thread starter Robert123
- Start date
JackSmooth
Member
The finished oil will come out sterile wether the filter is or not.
Mechanics
New Member
Are most filters non sterile anyways when new?
JackSmooth
Member
Most filters I've seen are all sterile but the main one that comes to mind that isn't are the whatman zapcaps.
Mechanics
New Member
So I am buying the Foxx Autofil bottle cap 0.22. I am assuming or hoping there sterile . if not can u just run BA through and then waste the first 3-4 ml that comes through .
JackSmooth
Member
BA won't sterilize the filter just like it doesn't sterilize your gear. If that was the case then why would you filter after adding the BA?
No need to run BA through it. You'll be good man.
Mechanics
New Member
Yeah I was asking cause I wasn't sure .
Just trying to learn as much as possible .
Just trying to learn as much as possible .
JackSmooth
Member
If you want to try sterilizing them I believe @Docd187123 uses a pressure cooker to sterilize his zapcaps. I'm sure he can elaborate on this method as I have no experience with using a pressure cooker for that purpose.
Johne71
Member
A non-sterile filter will not produce a sterile product. If the exit side of your filter has lint or any shit on it at all it will be in your product. You need a sterile filter to get what you need. It's like putting a non-sterile need on a sterile filter. I recommend getting individually sealed 0.22micron Whatmans.
JackSmooth
Member
We're speaking of non sterile filters straight out of the packaging. As long as the media bottle you're filtering into is sterile then your oil will not be contaminated.
I do use zapcaps but I do not use a pressure cooker on them.
Johne71
Member
I have to disagree. Your bottle is only sterile if all components pass through sterile components. A package of loose filters could have a lot of dust (as an example) collected on the side you will put your sterile needle on. You will simply push that possible dust directly into a sterile vial. Why use a sterile needle if the filter isn't sterile?
I worked in a micro lab for years. I don't recommend it. That is the sole purpose of sterile, individually wrapped filters.
JackSmooth
Member
Must've been something else I read. Vials maybe?
Auto claving stoppers is ok. I use dry heat for vials
this is what the people from medlabsupply said to me when I asked them the same question a week ago.
In regards to sterility, which is a very strong word, ultimately , something is either sterile, or not. There really are no degrees of sterility. Hospitals are careful about using this term at times, as well. We have sterile vials, we sell, but they come to us, From the manufacturer, completely sealed, un,opened, and are sold as such. We also have the COA s, for this product.
As far as the filters, there are two terms working here , Filtering out bacteria, which is the case with the filtration process. The filters that you received, are completely sealed, and protected from external surroundings. Even on the package from the supplier, it states they are not sterile. Once opened, anything could be subject to the air. However, the filtering process aims at filtering out , from said solution, potential bacteria. The filtering industry, is doing just that , filtering out, from said product, for usage. Your filters are coming to you, completely sealed .
Hopefully, this will help clear up, both terms, and the goal, achieved , for these processes.
In regards to sterility, which is a very strong word, ultimately , something is either sterile, or not. There really are no degrees of sterility. Hospitals are careful about using this term at times, as well. We have sterile vials, we sell, but they come to us, From the manufacturer, completely sealed, un,opened, and are sold as such. We also have the COA s, for this product.
As far as the filters, there are two terms working here , Filtering out bacteria, which is the case with the filtration process. The filters that you received, are completely sealed, and protected from external surroundings. Even on the package from the supplier, it states they are not sterile. Once opened, anything could be subject to the air. However, the filtering process aims at filtering out , from said solution, potential bacteria. The filtering industry, is doing just that , filtering out, from said product, for usage. Your filters are coming to you, completely sealed .
Hopefully, this will help clear up, both terms, and the goal, achieved , for these processes.
Johne71
Member
Analytical labs often purchase non-sterile filters just for the use of filtering out potential particles or debris they don't want passing through their HPLCs. If I'm sticking something in my leg or ass it will be a sterile filter 100% of the time.
The simple question to ask Medlab is "if I'm filtering something for human injection do you recommend sterile or non-sterile?" The answer should be more clear. I'm just trying to keep you safe. Do what you will with this information.
J
The simple question to ask Medlab is "if I'm filtering something for human injection do you recommend sterile or non-sterile?" The answer should be more clear. I'm just trying to keep you safe. Do what you will with this information.
J
Yeah man I was an asshole to that lady. Lol. I'm going to get a glass Buchner filter setup and just be done with it.
Johne71
Member
She also recommends you inject with a 16g. LOL. Got to be nice to those Reps. 
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