peptide shelf life after reconstitution.

[Keilani]

There is no comparison between pharma and UGL peptides.

Most pharma peptides have 3+ ingredients to prevent degradation, UGL's usually have jack shit. On top of that, PH, a MAJOR factor in the rate of degradation and carefully controlled in pharma products is random in UGL.

Then you've got stupidly high concentrations, like 20mg Sema, which speeds degradation even more.

No test on a UGL peptide can be applied to another vendor or batch. There are simply too many variables. It's not like carefully formulated pharma that's identical from batch to batch, without fail.

All you can do is control the factors we know help preserve all peptides, and try to use a vial up as quickly as possible after reconstitution.

It's not always possible. I can't use up a vial of MT-II quickly, but with a cryofreezer I may start to "aliquot" half of it, which is what labs do with portions of reconstituted peptide that won't be used for a while, rather than keep it in the refrigerator for months.

And may I ask two questions as a newbie.
How would you keep your Tirzepatide before Reconstitution?
And if you get 20mg Tirz, would you reconstitute it with just one ml of bac water?
Thank you!

 

[Anat]

And may I ask two questions as a newbie.
How would you keep your Tirzepatide before Reconstitution?
And if you get 20mg Tirz, would you reconstitute it with just one ml of bac water?
Thank you!

He’s currently banned, so he can’t reply to you. You can store your Tirz in powder form without bac water, just keep it in the fridge - many people do that, myself included. As for how much bac water you need, it depends on your dosage. According to @Ghoul, in medical studies they used about 0.5 ml per dose. For example, you have 20 mg Tirz and your weekly dose is 5 mg, you’d add 2 ml of bac water. Then on a 100-unit insulin syringe, 50 units would be your 5 mg dose.

You can also download “peptidecalc” on your phone to calculate dosages - it’s simple and straightforward.

 

[Keilani]

He’s currently banned, so he can’t reply to you. You can store your Tirz in powder form without bac water, just keep it in the fridge - many people do that, myself included. As for how much bac water you need, it depends on your dosage. According to @Ghoul, in medical studies they used about 0.5 ml per dose. For example, if your weekly dose is 5 mg, you’d add 2 ml of bac water. Then on a 100-unit insulin syringe, 50 units would be your 5 mg dose.

You can also download “peptidecalc” on your phone to calculate dosages - it’s simple and straightforward.

Thank you!!
Do you think (i believe so) that the compound would have suffered If I have stored it out if the fridge out of direct light for the past month or so?

 

[zpped]

Thank you!!
Do you think (i believe so) that the compound would have suffered If I have stored it out if the fridge out of direct light for the past month or so?

cabinet is fine for a couple months. fridge is good for a year, freezer is good for many years as a rule of thumb. colder is always better when it's in lyophilized (powder) form.

 

[Keilani]

cabinet is fine for a couple months. fridge is good for a year, freezer is good for many years as a rule of thumb. colder is always better when it's in lyophilized (powder) form.

Thank you! Thats a relief! Will put on the fridge now!

 

[Anat]

Thank you!!
Do you think (i believe so) that the compound would have suffered If I have stored it out if the fridge out of direct light for the past month or so?

You gain nothing but risk more damage to your peptides by storing them in a home freezer. It's like telling people it's fine to freeze and thaw your peptides multiple times a day, vs storing them in a refrigerator. where they'll have near zero degradation over the course of years.

Calling that "hysterics" doesn't compensate for the fact you're talking out of your ass about a subject you know nothing about, repeating "common knowledge" that'll cause harm to the compounds people think they're preserving by following that bad advice.

Here's the most unstable pharmaceutical peptide. The "worst case scenario" in terms of temperature related peptide degradation. Sema, Tirz, and HGH are all far more stable, demonstrating acceptable medical effectiveness even at storage temps over 100f for a month.

The orange line shows remaining purity after 3 years of storage.

In a certified ANSI lab freezer, which never exceeded -20c by even a single degree (the small amount of temp variance was colder, never warmer), vs refrigerator temperature, 4c, or 40F, the amount of purity lost was less than 0.5%.

View attachment 287849

So at best, under ideal conditions, at a temp most home freezers are unlikely to ever reach, and rise by 10-15+ degrees dozens of times every day as it cycles, you might save 0.5% purity over 3 years vs the refrigerator. But what actually happening in a home freezer is worse, because those freezer temp swings cross over the freeze/thaw point of the moisture in the peptide.

A vegetable kept frozen is ok after a year in so called "deep freeze", but thaw and refreeze it even once it turns to mush from the damage of water crystallization. It's far preferable to not allow peptide freezing to ever occur, unless you can guarantee it stays below -20c continuously, and you can't do that in a home freezer.

By the way, here's a pharma peptide that's far closer to the temperature sensitivity profile of GLP drugs. How much more purity do you preserve over 3 years in a $30,000 lab freezer at -20c vs refrigerator temps? (hint: none). So why are we risking freeze/thaw damage by throwing it a home freezer again?

View attachment 287850

So yeah, I know it hurts to realize you've been doing it wrong, and accomplishing the opposite of what you thought you were, but the proper response is to change what you're doing based on new info.

View attachment 287851

This is @Ghoul quote on storing GLP-1 in research - I hope it helps.

 

[Keilani]

This is @Ghoul quote on storing GLP-1 in research - I hope it helps.

So fridge it is!

 

[UncleBuns]

Here's what I know based on Jano tests:

All these time frames are assuming that once reconstitution happens, the vial is stored in a refrigerator.

HCG should be used within about 40 days after reconstitution and then the degradation becomes more rapid. From reconstitution to 40 days degradation is minimal to none. From 40 days to 60 days you get about a 30% loss. I don't know the rate of degradation past 60 days, but I assume it stays accelerated.

Recombinate HCG can be reconstituted, frozen, thawed, no problem.

Urine derived HCG that is reconstituted, frozen than thawed is severely degraded. About 40% or more.

GH once reconstituted is good for 14 days and only sees a little degradation up to 21 days. GH once reconstituted and then Frozen is extremely degradated.

Tirz and Sema once reconstituted are good for a long time. I don't know how long it would take for it to start degrading but well beyond the reasonable amount of time most people will use it. They can also be frozen after reconstitution and thawed.

BPC and TB 500 are very stable after reconstitution whether they are refrigerated or left at room temp. They see no degradation at the 60-day mark with either storage method.

In other peptides, I don't know, but because GH and HCG are some of the larger and more complicated peptide chains they likely degrade the fastest and easiest. Generally speaking the smaller the peptide the more stable it will be, but without doing real testing it's just guesswork.

 

[Keilani]

Here's what I know based on Jano tests:

All these time frames are assuming that once reconstitution happens, the vial is stored in a refrigerator.

HCG should be used within about 40 days after reconstitution and then the degradation becomes more rapid. From reconstitution to 40 days degradation is minimal to none. From 40 days to 60 days you get about a 30% loss. I don't know the rate of degradation past 60 days, but I assume it stays accelerated.

Recombinate HCG can be reconstituted, frozen, thawed, no problem.

Urine derived HCG that is reconstituted, frozen than thawed is severely degraded. About 40% or more.

GH once reconstituted is good for 14 days and only sees a little degradation up to 21 days. GH once reconstituted and then Frozen is extremely degradated.

Tirz and Sema once reconstituted are good for a long time. I don't know how long it would take for it to start degrading but well beyond the reasonable amount of time most people will use it. They can also be frozen after reconstitution and thawed.

BPC and TB 500 are very stable after reconstitution whether they are refrigerated or left at room temp. They see no degradation at the 60-day mark with either storage method.

In other peptides, I don't know, but because GH and HCG are some of the larger and more complicated peptide chains they likely degrade the fastest and easiest. Generally speaking the smaller the peptide the more stable it will be, but without doing real testing it's just guesswork.

This is great amd very helpful! Thank you so much!
I have always kept my powder BPC out fridge, but i think considering ill be putting the Tirz into fridge, i may as well put both there!

Thanks again everyone for the help!

 

[UncleBuns]

This is great amd very helpful! Thank you so much!
I have always kept my powder BPC out fridge, but i think considering ill be putting the Tirz into fridge, i may as well put both there!

Thanks again everyone for the help!

I know this information is just me typing things out, but there are Jano tests to prove everything I've written. The testing is part of a private group though, so I am not allowed to share the results outside of the group

 

[FuriousGrill]

I know this information is just me typing things out, but there are Jano tests to prove everything I've written. The testing is part of a private group though, so I am not allowed to share the results outside of the group

ِAre you aware of any testing done on frozen GH powder? I have 8000 units in my fridge and I'm still unsure on how to store it well... I'm planning it to use it over the next 4-5 years.

 

[UncleBuns]

ِAre you aware of any testing done on frozen GH powder? I have 8000 units in my fridge and I'm still unsure on how to store it well... I'm planning it to use it over the next 4-5 years.

Put it in a freezer

 

[Letits now]

Thank you!!
Do you think (i believe so) that the compound would have suffered If I have stored it out if the fridge out of direct light for the past month or so?

One thing we need to remember is the producers don’t store these in a fridge. And we still hit purity and weight after they store, mail it and then we test it. Even the hgh. So you should be fine.

 

[FuriousGrill]

One thing we need to remember is the producers don’t store these in a fridge. And we still hit purity and weight after they store, mail it and then we test it. Even the hgh. So you should be fine.

The question remains though, we do not have data on how much degradation occurs if those peptides are stored outside vs the fridge vs the freezer, no? Particularly with GH.

 

[Letits now]

The question remains though, we do not have data on how much degradation occurs if those peptides are stored outside vs the fridge vs the freezer, no? Particularly with GH.

There may be a test going on discord comparing this exact thing right now.

 

[zpped]

The question remains though, we do not have data on how much degradation occurs if those peptides are stored outside vs the fridge vs the freezer, no? Particularly with GH.

I'm only aware of that specific data for tirz.

 

[FuriousGrill]

There may be a test going on discord comparing this exact thing right now.

Are you aware of any preliminary results? This is amazing.

I'm only aware of that specific data for tirz.

Mind sharing?

 

[zpped]

Are you aware of any preliminary results? This is amazing.

Mind sharing?

Sorry I thought I got back to this already. I can't show the tests themselves because of the group they come from from. But over a year and a half all three storage locations did very well. (within 10% of original mass and <1% purity change). The numbers are so close and within the margin of error of any given test.

 

[FuriousGrill]

Sorry I thought I got back to this already. I can't show the tests themselves because of the group they come from from. But over a year and a half all three storage locations did very well. (within 10% of original mass and <1% purity change). The numbers are so close and within the margin of error of any given test.

This is very useful information; thanks for sharing!

 

[jaxked]

Random question.. what are the requirements to post in a sponsor thread in the underground section?