Poll: Who filters their peptides?

[Thorshammer]

This is not a decision to be made by following the herd.

If someone asked "Who's quit smoking cigarettes to protect against cance?"" in 1940, or "Who's decided to avoid trans fats to reduce their risk of having a heart attack?" in 1980, and followed the majority, how would that have worked out?

Weigh the potential risks against the "burden" of filtering (it's really nothing once you get on the habit), and make your own choice, because whatever the consequences are, in 1 month or 20 years, will be yours alone.

This was perfectly said. When it comes to sterility in this game the bar is so low. You gotta do your own research and make educated decisions. The vast majority are careless or ignorant of the consequences.

 

[Elektrobot]

Wasn't sure which filtering thread to put this in so I randomly chose this one

Found these filters that I don't remember seeing mentioned in any of the filtering threads

Pricing seems pretty good for a domestic legit biotechnology supplier

PVDF, PES and PTFE. their PVDF go down to 0.10um but not sure if that is beneficial for our use.

Syringe Filter – UltraCruz® | SCBT - Santa Cruz Biotechnology

UltraCruz® Syringe Filter is suitable for filtering aqueous and mild organic solutions such as buffers, tissue culture media, or additives

www.scbt.com

 

[Gb9]

How is it cost effective if you need to pin 5 different peptides including hgh every day? That's a lot of PES filters and filtering by the end of the month. Unless you have a method that you can share to filter more cost effective.

Could you filter all your peps at once? For example, pre-fill your syringes for 1-2 week at a time? I’m asking because I don’t know; never filtered, but considering if I could do that.

 

[Gb9]

But the real question is. Are you really reducing harm in the way you think you are, or is it just the perception of being protected by a filter? I think the danger lies in that people think it gives them full protection against contamination by filtering it.

A filter is not a fix for everything bad. A 0.22 µm filter can remove live bacteria from solutions, but it cannot remove endotoxins/pyrogens, dissolved impurities, solvents, heavy metals, or truncated peptide byproducts, the main issues in this space. Endotoxins are smaller than the pores and heat-stable, so they sail right through.

So all you're doing is removing those which may cause an infection or inflamation.

Not blocked are:
Endotoxins
Dissolved toxins, solvents, or heavy metals
Incorrect or degraded peptide molecules
Some virusses

And theoretically, there’s no need to filter for bacteria because the BAC will kill it? So zero benefits to filter?

 

[Sera]

Could you filter all your peps at once? For example, pre-fill your syringes for 1-2 week at a time? I’m asking because I don’t know; never filtered, but considering if I could do that.

You use a sterile empty vial and you filter the entire vial at once.

 

[Barney67]

And theoretically, there’s no need to filter for bacteria because the BAC will kill it? So zero benefits to filter?

BacterioSTATIC water not bacteriocidal. I would argue filtering is more important than BAC use because if you use sterile syringes and needles/pen needles everytime, contamination is virtually negligible.

https://www.researchgate.net/publication/38067784_Microbial_contamination_of_syringes_during_preparation_The_direct_influence_of_environmental_cleanliness_and_risk_manipulations_on_end-product_quality

This study shows that as long as you don’t touch anything/break sterility, contamination rates were 0%. This along with fridge temps is good enough.

 

[Gb9]

BacterioSTATIC water not bacteriocidal. I would argue filtering is more important than BAC use because if you use sterile syringes and needles/pen needles everytime, contamination is virtually negligible.

https://www.researchgate.net/publication/38067784_Microbial_contamination_of_syringes_during_preparation_The_direct_influence_of_environmental_cleanliness_and_risk_manipulations_on_end-product_quality

This study shows that as long as you don’t touch anything/break sterility, contamination rates were 0%. This along with fridge temps is good enough.

Thanks for the info. Considering filtering, seems easy enough to do:

Reconstitute with BAC in original vial.
Use 3ml syringe to draw up all solution.
On unused vial, add filter.
Push solution thru filter into new vial.
Now it’s ready to go, dose out as normal.

Any specific new vials I should use? Seems like if you don’t get a super reputable source for new vials, you’re actually adding potential contamination.

 

[Sera]

Thanks for the info. Considering filtering, seems easy enough to do:

Reconstitute with BAC in original vial.
Use 3ml syringe to draw up all solution.
On unused vial, add filter.
Push solution thru filter into new vial.
Now it’s ready to go, dose out as normal.

Any specific new vials I should use? Seems like if you don’t get a super reputable source for new vials, you’re actually adding potential contamination.

Here ya go mate

Ultra Spec Sterile 2ML (13mm neck) Clear Sealed Glass Vials

In order to receive vials in sealed shrink wrap boxes, please order in multiples of 25 Ultra Spec Sterile 2ML-13mm Clear Sealed Glass Vial, Qty 1 ULTRA SPEC sterile depyrogenated vials are assembled with Teflon coated non-latex chlorobutyl rubber stoppers (conforming with USP<381>) and vials...

www.medical-and-lab-supplies.com

 

[Barney67]

Thanks for the info. Considering filtering, seems easy enough to do:

Reconstitute with BAC in original vial.
Use 3ml syringe to draw up all solution.
On unused vial, add filter.
Push solution thru filter into new vial.
Now it’s ready to go, dose out as normal.

Any specific new vials I should use? Seems like if you don’t get a super reputable source for new vials, you’re actually adding potential contamination.

A popular source that has tested well for sterility before is Ks-Tek. They’re on both Amazon and AliExpress.

look up “peptide test filtering” on Youtube, and the first result should be a ~16 minute video by a channel called Peptide Test. It’s a good comprehensive video. Keep in mind that you do not need to add a filter to the venting needle like he does in the video.

 

[MrBillyMG]

A popular source that has tested well for sterility before is Ks-Tek. They’re on both Amazon and AliExpress.

look up “peptide test filtering” on Youtube, and the first result should be a ~16 minute video by a channel called Peptide Test. It’s a good comprehensive video. Keep in mind that you do not need to add a filter to the venting needle like he does in the video.

These are what I use and I’ve had success on the 2 I’ve used so far. Got them off Amazon..

 

[Jemifus]

It’s mostly negligible. Split your BAC dose into 2. Reconstitute your peptide with the first partition and filter those, then use the second partition to flush the filter and wash out whatever peptides remain. Then you can do an air flush to get the last bits of BAC from the filter.

Can you describe the logistics of partitioning the bac? Do you draw the second dose of bac from the bac water vial after pushing the first reconstituted portion through the filter?

Does it look something like this?
1. recon with half the bac dose
2. draw into syringe
3. take off needle, add filter, put needle back on
4. push reconned solution through filter
5. pull syringe off filter, add fresh needle
6. pull second half of bac from original bac water vial
7. remove second needle, attach syringe to original filter and needle
8. push second half of bac through the filter?

I'm also finding i really need to use a vent needle, at least with the 4mm PES filters. The ks-tek vials aren't vacuum and with the filter on i can't alternate between drawing air and pushing solution.

 

[Sera]

Can you describe the logistics of partitioning the bac? Do you draw the second dose of bac from the bac water vial after pushing the first reconstituted portion through the filter?

Does it look something like this?
1. recon with half the bac dose
2. draw into syringe
3. take off needle, add filter, put needle back on
4. push reconned solution through filter
5. pull syringe off filter, add fresh needle
6. pull second half of bac from original bac water vial
7. remove second needle, attach syringe to original filter and needle
8. push second half of bac through the filter?

I'm also finding i really need to use a vent needle, at least with the 4mm PES filters. The ks-tek vials aren't vacuum and with the filter on i can't alternate between drawing air and pushing solution.

There’s no need to do it, the amount that remains in the filter is negligible if you use over 1mL to reconstitute. Ideally 2mL.

 

[Photon]

Wasn't sure which filtering thread to put this in so I randomly chose this one

Found these filters that I don't remember seeing mentioned in any of the filtering threads

Pricing seems pretty good for a domestic legit biotechnology supplier

PVDF, PES and PTFE. their PVDF go down to 0.10um but not sure if that is beneficial for our use.

Syringe Filter – UltraCruz® | SCBT - Santa Cruz Biotechnology

UltraCruz® Syringe Filter is suitable for filtering aqueous and mild organic solutions such as buffers, tissue culture media, or additives

www.scbt.com

There aren't alot of options for them.
You want them sterile, which limits them to only this.
Cobetter (US Warehouse only) or Ks-Tek (AliExpress) is still better imo.

 

[RandGuy]

As others have noted, one can eliminate need for new vials by backfilling sterile syringes with filtered product, and refrigerating the syringes for later use. I still use a procedure similar to this:

R

Post in thread 'The FDA makes the case for filtering peptides'

May 9, 2025

Here's procedure I've settled on*... Starting with 10 IU vial from K4L, add 1.1 ml** BAC water using a 1 ml Luer Lock syringe with 5/8" needle (I found the 1.5" needles to be clumsy to work with). Add another 1.1 ml of air to reduce vacuum. After dissolution, use same 1 ml syringe to draw out 1.1 ml of reconstituted fluid. Replace the 5/8" needle with 0.22 um filter, push fluid into the filter, filling outlet. Then backfill 4 insulin syringes (I use 0.3 ml syringes) with 0.2 ml (20 units) each (point syringe at downward angle, and press filter outlet into syringe top to ensure fluid...

• RandGuy

 

[Sera]

As others have noted, one can eliminate need for new vials by backfilling sterile syringes with filtered product, and refrigerating the syringes for later use. I still use a procedure similar to this:

R

Post in thread 'The FDA makes the case for filtering peptides'

May 9, 2025

Here's procedure I've settled on*... Starting with 10 IU vial from K4L, add 1.1 ml** BAC water using a 1 ml Luer Lock syringe with 5/8" needle (I found the 1.5" needles to be clumsy to work with). Add another 1.1 ml of air to reduce vacuum. After dissolution, use same 1 ml syringe to draw out 1.1 ml of reconstituted fluid. Replace the 5/8" needle with 0.22 um filter, push fluid into the filter, filling outlet. Then backfill 4 insulin syringes (I use 0.3 ml syringes) with 0.2 ml (20 units) each (point syringe at downward angle, and press filter outlet into syringe top to ensure fluid...

• RandGuy

Waste 27% of your hgh and have to reconstitute and filter every 4 days, or buy sterile vials and filter once every 2 weeks or so and waste ~none ? Hmm.

Although your method admittedly would leave you with sharper needles, which is a nice bonus.

 

[RandGuy]

Waste 27% of your hgh and have to reconstitute and filter every 4 days, or buy sterile vials and filter once every 2 weeks or so and waste ~none ? Hmm.

Although your method admittedly would leave you with sharper needles, which is a nice bonus.

I'm only injecting about 2IU every other day (anti-aging), so 1 vial of 10 IU gets me 8 days, and I wouldn't want to store reconstituted HGH longer than that. Plus, the K4L HGH is relatively inexpensive, so wasting 20% on the filter (I've improved the technique a bit) is not an issue.

 

[Barney67]

I'm only injecting about 2IU every other day (anti-aging), so 1 vial of 10 IU gets me 8 days, and I wouldn't want to store reconstituted HGH longer than that. Plus, the K4L HGH is relatively inexpensive, so wasting 20% on the filter (I've improved the technique a bit) is not an issue.

Look up pens, quite a nice QoL improvement.

 

[KOArtist]

Filtered my first 10ml off gh today, my vote has been changed

 

[Ateam2023]

Filtered my first 10ml off gh today, my vote has been changed

Like did you filter a whole kit into a 10 ml vial?

 

[KOArtist]

Like did you filter a whole kit into a 10 ml vial?

There’s 100iu in it, so sure, a traditional small kit (this only took less than two vials, if you know what I green) It was a bit of a fučking grip workout too, gonna squeeze with 23g next time probably