Possible methods other than shipping things for hplc?

[achba]

Possible method for determining the rough concentration of oils?
Step one is to draw about an mL of oil and then cooling it which may or may not cause precipitation. Then in the same syringe adding the same amount of ethanol, dioaxane, chloroform, or ether (ethanol or isopropanol would be the best choices) and mixing. Once the layers seperate, repeat 2 more times. Then add a smaller amount, remove that layer, and add water and see if there is any precipitation that isnt oil, and repeat until you dont see any more. Next, add the solvent to a different container and cool it again, and remove any visible oil. Then dry the solution, and weigh the powder for a rough estimation of concentration? It should be much more soluble in those solvents than cold oil.

Next could you try reagents? I've read a few papers about concentrated sulfuric acid, mandelins reagent, etc all turning different compounds either one colour or changing from one colour to another over time. I understand that it is subjective what colour it is but it should help you make an educated guess as to whether it is what you paid for or not.

 

[BigTomJ]

Possible method for determining the rough concentration of oils?
Step one is to draw about an mL of oil and then cooling it which may or may not cause precipitation. Then in the same syringe adding the same amount of ethanol, dioaxane, chloroform, or ether (ethanol or isopropanol would be the best choices) and mixing. Once the layers seperate, repeat 2 more times. Then add a smaller amount, remove that layer, and add water and see if there is any precipitation that isnt oil, and repeat until you dont see any more. Next, add the solvent to a different container and cool it again, and remove any visible oil. Then dry the solution, and weigh the powder for a rough estimation of concentration? It should be much more soluble in those solvents than cold oil.

Next could you try reagents? I've read a few papers about concentrated sulfuric acid, mandelins reagent, etc all turning different compounds either one colour or changing from one colour to another over time. I understand that it is subjective what colour it is but it should help you make an educated guess as to whether it is what you paid for or not.

cheaper and easier to just get HPLC done and then its no guessing, nothing subjective.

 

[achba]

cheaper and easier to just get HPLC done and then its no guessing, nothing subjective.

It would cost way less than getting hplc done. Mandelins reagent is like 20 dollars, high concentration isopropanol is like 3 dollars, water is free.

 

[BigTomJ]

It would cost way less than getting hplc done. Mandelins reagent is like 20 dollars, high concentration isopropanol is like 3 dollars, water is free.

and whats your time worth to conduct all the tests and processes?

what margin of error would you expect?

how much would getting an accurate mg scale cost you and all the other equipment and materials?



its just a lot of effort, to maybe save a few bucks, only to have a guess at the compound or potency.

 

[achba]

and whats your time worth to conduct all the tests and processes?

what margin of error would you expect?

it would take 20 minutes to do, I haven't found my margin of error but I could take the powder, redissolve it in a carrier oil, and carry it out again to find margin of error. its no going to be as accurate as hplc but it should give a rough idea of the amount and what it could be.

 

[Rido]

You can ask @janoshik which $20,000 machine(used) he would recommend for you to purchase and save money, among other things


or you can utilize pirateship and just send it off to him for about 50 dollars+his testing fees

 

[janoshik]

You can ask @janoshik which $20,000 machine(used) he would recommend for you to purchase and save money, among other things


or you can utilize pirateship and just send it off to him for about 50 dollars+his testing fees

Shh,

I want to see the guy waiting for the miscible solvents to separate.

Won't even bother mentioning fancy expensive terms like partition coefficients and so.

 

[achba]

Shh,

I want to see the guy waiting for the miscible solvents to separate.

Won't even bother mentioning fancy expensive terms like partition coefficients and so.

they do seperate

 

[janoshik]

they do seperate

Sure thing bud.

 

[achba]

Sure thing bud.

literally just did it and then added water to the top layer and it turned opaque instantly with a small glob of oil collecting at the bottom, everything redissolved when it was heated but the oil glob was still there

 

[vivendi]

Just curious about this. I know it is a rough extraction method and may not yield pure testosterone or an accurate concentration.

I can also imagine that benzyl alcohol and benzyl benzoate are hard (maybe impossible?) to separate.

But doesn’t it give an idea about the concentration of the gear? I mean, if it’s underdosed, wouldn’t you be able to tell using a method like this?

Don’t get me wrong. Using quality equipment would always be best. Just wondering if this method could be used as an indicator.

 

[janoshik]

Just curious about this. I know it is a rough extraction method and may not yield pure testosterone or an accurate concentration.

I can also imagine that benzyl alcohol and benzyl benzoate are hard (maybe impossible?) to separate.

But doesn’t it give an idea about the concentration of the gear? I mean, if it’s underdosed, wouldn’t you be able to tell using a method like this?

Don’t get me wrong. Using quality equipment would always be best. Just wondering if this method could be used as an indicator.

Yes, but I'd bet double blind study would find feelz™ to be both more accurate and precise method of analysis.

 

[Rido]

literally just did it and then added water to the top layer and it turned opaque instantly with a small glob of oil collecting at the bottom, everything redissolved when it was heated but the oil glob was still there

IDK man, I know I am giving you a hard time but I just feel like it simpler, more accurate to send it in for testing.

if you want to screw around and compare it with testing service for fun, then that would be cool.

I would take your own personal test results with a grain of salt. just saying

 

[Stanfordpharma1]

Yes, but I'd bet double blind study would find feelz™ to be both more accurate and precise method of analysis.

This is how I Test all my raws. Feelz. I put a pinch between my index finger and my thumb and roll it around and I can, with extreme accuracy, "feel" what product it is and the purity.

 

[Stanfordpharma1]

Shh,

I want to see the guy waiting for the miscible solvents to separate.

Won't even bother mentioning fancy expensive terms like partition coefficients and so.

Shoot, if the machine is only $20,000 I'd be so happy to learn how to do this. I mean, if it wasn't rocket science. My "aged" brain has lost its ability to learn new things well. But I think I would find this fascinating.

 

[Stanfordpharma1]

Shh,

I want to see the guy waiting for the miscible solvents to separate.

Won't even bother mentioning fancy expensive terms like partition coefficients and so.

Plus, I could hplc test all my own products, haha. That wouldn't go over very well, lol.