Love love
love that you are pushing on the science, genuinely. Respectfully, some of the thread is getting ahead of the methods and treating a complex analytical question like it’s binary. In practice, variables like solvent choice (methanol adducts), pH, sample prep, column chemistry, gradient, internal standards, and calibration all influence what you “see.” That’s why two HPLC results can diverge without anyone acting in bad faith.
We’re here for the scrutiny, just want to avoid turning hypotheticals into headlines that spook people. Please guys, let’s stay methodical, compare apples to apples, and let the data speak.
I know he has fun in here but
@sionnach really knows his stuff. Would love to hear what he thinks.