Questionable sterilisation

[Nezz]

Hey fellas

I have recently switched to media bottles to store my oils and I noticed soemthing off.

my process:
Soak bottles and caps in 70% iso for 15min.

add distilled water to bottle and recap and shake the fuck out of it. Empty repeat 3 times.

Cover tops of bottles with foil that’s been dipped in iso and dipped again in distilled water. Poke small hole.

bake at 210c for 90min and left to cool inside brand new oven.


afterwards I inspected them I noticed small burn marks (brown spots, only slightly). Thinking back I only really cleaned inside and not outside of bottle. Is there a chance this is why there is burn spots on them, and won’t effect inside?

For piece of mind I will go though the process again and see what outcome is. Anyone with some insight or what happened? Or do burn marks occur on places intact with oven rails (I think it may be in same place but no consistent lines down bottle just small dot (like a drip)

 

[Jet Labs]

Brown dots are usually from tiny amounts of oil that's burnt onto the glass and they shouldn't be an issue for you, but I'll suggest a better way to clean/sterile your glassware.

Scrub glassware with new sponge and for media bottles get a bottle brush and use lots of Sunlight brand as it cleans oils very well.

Rinse media bottles/caps under hot tap water thoroughly then place into pressure cooker using distilled water running at 15psi for 45 minutes then allow to dry in clean rack for a bit. Caps should be wrapped in clean tinfoil right out of the pressure cooker.

Cover ends with cleaned tinfoil and poke a few small toothpick size holes and bake at 500f for 3-4 hours. Bake caps in tinfoil for 1 hour at 100f.

Oh and brown spots on outside of glassware has no impact on sterility.

 

[D Zero]

Cleaning vials, pressure cook in distilled water for 40 minutes and bake at 400 for 35 minutes is all I use. The baking is just to get rid of moisture the pressure cooker is gonna insure sterility.

I wouldn't waste time with alcohol except for stoppers and sterilizing your gloved hands.

The trick is to prepare a metal tray (large bake pan) that isn't touched and covered until you get ready to fill the vials. Treat it like they were surgical instruments ready for the OR. Wear your N95 mask, gloves, long sleeves or lab coat with no fibers.

 

[Nezz]

Cleaning vials, pressure cook in distilled water for 40 minutes and bake at 400 for 35 minutes is all I use. The baking is just to get rid of moisture the pressure cooker is gonna insure sterility.

I wouldn't waste time with alcohol except for stoppers and sterilizing your gloved hands.

The trick is to prepare a metal tray (large bake pan) that isn't touched and covered until you get ready to fill the vials. Treat it like they were surgical instruments ready for the OR. Wear your N95 mask, gloves, long sleeves or lab coat with no fibers.

Might be a obvious answer but how much water do you know to add into cooker?

 

[D Zero]

Might be a obvious answer but how much water do you know to add into cooker?

Glassware needs to be submerged in distilled water. It's not just sterilizing, it's also part of the cleaning process.

 

[xmilar]

Brown dots are usually from tiny amounts of oil that's burnt onto the glass and they shouldn't be an issue for you, but I'll suggest a better way to clean/sterile your glassware.

Scrub glassware with new sponge and for media bottles get a bottle brush and use lots of Sunlight brand as it cleans oils very well.

Rinse media bottles/caps under hot tap water thoroughly then place into pressure cooker using distilled water running at 15psi for 45 minutes then allow to dry in clean rack for a bit. Caps should be wrapped in clean tinfoil right out of the pressure cooker.

Cover ends with cleaned tinfoil and poke a few small toothpick size holes and bake at 500f for 3-4 hours. Bake caps in tinfoil for 1 hour at 100f.

Oh and brown spots on outside of glassware has no impact on sterility.

Do you place the vials in a autoclave bag? or just as is inside the pressure cooker?