Readalots Enhanced Testing

[bigMoJo]

Quite ironic that, don’t you think?

Yes. Although, I would argue about "independent evidence." However, that's for another forum.

Personally, it makes me "merry" and proud that those living in diverse countries can unite and celebrate holidays together. We work together, we live together, we "serve" together... why not celebrate together.

Happy Holidays brother!

 

[Zebedee]

Personally, it makes me "merry" and proud that those living in diverse countries can unite and celebrate holidays together. We work together, we live together, we "serve" together... why not celebrate together.

Happy Holidays brother!

Yes mate, there’s often more that unites us than separates us as communities & it’s in this community’s interest to recognise that & put aside that which divides us as we work towards our goal of harm reduction.

Merry Xmas mate, hope you ‘n yours have a good ‘un, as we say on this side of the pond.

 

[Zebedee]

The -ene functionality is applicable to the gonane core so my understanding is these impurities could be potentially present with any testosterone ester as the testosterone is synthesized prior to esterification. I would not think any one ester would be more prone to these impurities as the basic esterification step is same for all. Any organic chemists / interested parties please correct me if / where wrong.

My take would be a sample high in -ene impurities would be a poorly synthesized testosterone base material (poor testosterone selectivity).

My hypothesis: these impurities in their esterified form are slipping through HPLC and being picked up as Test ester API.

Cheers for that - my chemistry knowledge is pretty abysmal TBH.

Out of curiosity, do you know whether it’s theoretically possible that there’s something that would cause similarly false readings in an HLPC test for 17aa compounds? Just that many old skool guys are adamant that pharma oral AAS were much stronger back in the day & these claims are almost always dismissed as rosey tinted glasses etc because “tested 25mg dbol is 25mg dbol”.

 

[readalot]

Cheers for that - my chemistry knowledge is pretty abysmal TBH.

Out of curiosity, do you know whether it’s theoretically possible that there’s something that would cause similarly false readings in an HLPC test for 17aa compounds? Just that many old skool guys are adamant that pharma oral AAS were much stronger back in the day & these claims are almost always dismissed as rosey tinted glasses etc because “tested 25mg dbol is 25mg dbol”.

Great question. I'll get back to you with more color. Short answer is yes there are similar structural impurities for 17aa compounds but they are easier to resolve on HPLC as they don't have the carboxylic acid hanging off like the esterified Test.

 

[Exhortae]

Ver nice thread. Thanks readalot

I am concerned about endotoxin in ugl. Will probably test an oil of a big ugl in the next couple of weeks

 

[readalot]

Ver nice thread. Thanks readalot

I am concerned about endotoxin in ugl. Will probably test an oil of a big ugl in the next couple of weeks

Thank you.

 

[readalot]

Thank you very much for setting up the thread. Yes I can just as soon as I have the proper time. Got a lot going on this week but we should have a proper description of these tests before you get the results. Fantastic work Dirthand. I appreciate you.

Edit: I'll put the list here so everyone can see scope. I'll avoid an essay (haha) for now.

Special thanks to @janoshik for expanding the metals test for no additional charge! Very nice of him.

1. Hplc Purity - 120 usd
2. GCMS - 170 usd
3. Expanded metals list for standard list price (personally approved by Jano via xxxxxxxxx) - 60 usd
4. endotoxins - 120 usd
5. Residual solvent poor man's test (KF+loss on drying) - 60 usd
6. Shipping 45 usd
7. total - 575 usd

The residual solvents testing is mostly symbolic at this point as Jano and I agreed this won't tell us much. At some point a proper GCMS test with proper equipment would allow accurate characterization of residual solvents. The business case for the equipment just isn't there yet. But anyway this list is a start to get us all closer to USP standards.

Well I thought it was obvious and I was wrong. So let's add one more item to the list since BBBG wants to keep misrepresenting the list.

**Visible particulates.
No source should have these "floaters" in their finished oil. Make sure you ask them their SOP to ensure these particulates don't make it into the vial (vial, not vile). Unacceptable. Maybe they need the education.

** HPLC testing of AAS raws.
Vendors should tests their raws prior to using them to make finished products.

1. Hplc Purity - 120 usd
Identity and strength of API.

2. GCMS - 170 usd
Structural impurities along for the ride. Gives some insight into the purity of the raws used in a finished oil. Open question on purity of Test E and Test C raws.

3. Expanded metals list for standard list price - 60 usd
De-emphasized given the absence of any positive results to date. Intermittent surveillance still wise.

4. endotoxins - 120 usd
Cell wall debris from gram negative bacteria. Audit on sterility of UGL manufacturing process. Endotoxins pass through a 0.2 um filter.

5. Residual solvents
An appropriate vendor for GCMS characterization of class 1 and 2 residual solvents has not been identified for UGL products to date.

 

[Ateam2023]

Well I thought it was obvious and I was wrong. So let's add one more item to the list since BBBG wants to keep misrepresenting the list.

**Visible particulates.
No source should have these "floaters" in their finished oil. Make sure you ask them their SOP to ensure these particulates don't make it into the vial (vial, not vile). Unacceptable.

** HPLC testing of AAS raws.
Vendors should tests their raws prior to using them to make finished products.

1. Hplc Purity - 120 usd
Identity and strength of API.

2. GCMS - 170 usd
Structural impurities along for the ride. Gives some insight into the purity of the raws used in a finished oil. Open question on purity of Test E and Test C raws.

3. Expanded metals list for standard list price - 60 usd
De-emphasized given the absence of any positive results to date. Intermittent surveillance still wise.

4. endotoxins - 120 usd
Cell wall debris from gram negative bacteria. Audit on sterility of UGL manufacturing process. Endotoxins pass through a 0.2 um filter.

5. Residual solvents
An appropriate vendor for GCMS characterization of class 1 and 2 residual solvents has not been identified for UGL products to date.

SOP ? Excuse my lack of understanding

 

[Ateam2023]

SOP ? Excuse my lack of understanding

Sterile operations procedures

 

[readalot]

Sterile operations procedures

Standard operating procedures. Sorry.