Reconstitution

[Eddie.]

Best practice is to reconstitute only as needed, but don't use sooner than 15 minutes after the solution is visibly clear.

Personally I reconstitute all peptides the day before I intend to use them. This allows enough time to ensure even the most difficult to reconstitute parts of the peptide have time to fully rehydrate.

For HGH a minimum 1ml per 36iu, up to 5ml. Higher dilution rates are preferable when the vial will be kept in a reconstituted state for longer periods of time.

Interesting post, so if a vial is about to be used enough times (meaning like 3-4 IU a day from a 36 vial) is it best to use more bac water? Is this because of the "extra" BA from the bac water or due to water-powder ratio?

I think i've read some posts in another thread about minimum bac water to powder ratio but i can't remember the details. For a 10-12 IU vial that's about to be used 3 times, is 0,6ml bac water ok for reconstitution? Just to make things simple by drawing 0,2ml each time. Also, i don't see any point of injecting more bac water if it's not necessary.

 

[Ghoul]

Interesting post, so if a vial is about to be used enough times (meaning like 3-4 IU a day from a 36 vial) is it best to use more bac water? Is this because of the "extra" BA from the bac water or due to water-powder ratio?

I think i've read some posts in another thread about minimum bac water to powder ratio but i can't remember the details. For a 10-12 IU vial that's about to be used 3 times, is 0,6ml bac water ok for reconstitution? Just to make things simple by drawing 0,2ml each time. Also, i don't see any point of injecting more bac water if it's not necessary.

So you mean 12iu / 3 = 4iu dose

4iu x .04ml " .16ml.

.2ml is enough based on my theory, not below the somatropin dilution, and a good safety margin above it.

The issue with higher concentration again, is that packing peptides into too little water increases aggregation. Aggregates are wasted, ineffective peptides. And of course I won't repeat the immunogenicity issue with aggregates.

In addition, higher concentration makes it easier for more of the peptide to be quickly transported to lymph nodes, where they "train" the immune system to more effectively neutralize them.

 

[Eddie.]

So you mean 12iu / 3 = 4iu dose

4iu x .04ml " .16ml.

.2ml is enough based on my theory, not below the somatropin dilution, and a good safety margin above it.

The issue with higher concentration again, is that packing peptides into too little water increases aggregation. Aggregates are wasted, ineffective peptides. And of course I won't repeat the immunogenicity issue with aggregates.

In addition, higher concentration makes it easier for more of the peptide to be quickly transported to lymph nodes, where they "train" the immune system to more effectively neutralize them.

Ok, understood. Thanks!