Sterilization for stoppers and caps

[balco]

Yea me either. What i posted last is the best I found. dental autoclave

All the reviews I've read on Chinese generic autoclaves all say the same thing- "it lasted a 6 months and they won't honor warranty".

I think im just going to save up 2k and invest in a new american/european autoclave.

 

[Krampus]

How would you assemble the vials to maintain sterilization. Could you do it while its still in the autoclave bag and dry?

Basically, there's always more that could be done, but having sterile gloves and placing everything on a surface that has been cleaned by lysol in a dust free room will generally do just fine.

If you really want impeccable sterility you would need to set up a clean room. This would roughly be a room with higher air pressure than outside, full of air filtered through a hepa system, all surfaces sterilized, and would have a transition area entered through where you'd put on your clean suit.

Do you need to keep it away from the heated bottom? What would I use som type of stainless steel rack? Like a barbeque rack? to keep everything in this middle.

Yes, a stainless steel rack, just something to give it a few inches away from the bottom should be good.

 

[Krampus]

I can't find any 20 psi pressure cookers on Amazon, can you link me to one?

Edit: I just noticed that one is aluminum, I'll keep looking.

 

[Krampus]

Autoclave Overview

"Autoclaves use saturated steam under pressure of approximately 15 pounds per square inch to achieve a chamber temperature of at least 250°F (121°C) for a prescribed time—usually 30–60 minutes."

All of the autoclaves I've personally had ran at 20lbs, and I'd swear I've seen stainless pressure cookers that will do the same, but according to UCSD a 15lb cooker should work, just run it longer.


If you want to be sure your setup is good, run a spore test kit through. If it's sterile the spores don't grow.
https://www.painfulpleasures.com/bi...-kit-make-sure-your-autoclave-is-working.html

 

[Big dog]

For the vials stoppers and sluminum toppers? How do you put them together to maintain sterilzation?

I wrap a bunch in aluminum foil after they soak in alcohol and then I bake them. Glove up with medical gloves when capping.

 

[jJjburton]

Autoclave Overview

"Autoclaves use saturated steam under pressure of approximately 15 pounds per square inch to achieve a chamber temperature of at least 250°F (121°C) for a prescribed time—usually 30–60 minutes."

All of the autoclaves I've personally had ran at 20lbs, and I'd swear I've seen stainless pressure cookers that will do the same, but according to UCSD a 15lb cooker should work, just run it longer.


If you want to be sure your setup is good, run a spore test kit through. If it's sterile the spores don't grow.
Duo Spore® Biological Monitoring System Test Kit with Mail-In Culture Service

So if you use a auto clave its steam. But when using the pressure cooker do you let the stuff under the water line? Or does the water go above the vials and stoppers. Of course in the auroclave bag.

 

[Krampus]

So if you use a auto clave its steam. But when using the pressure cooker do you let the stuff under the water line? Or does the water go above the vials and stoppers. Of course in the auroclave bag.

You'll probably do best to have it above the waterline. Though, technically it doesn't matter if it's in the water or out. In terms of sterilization, it will still be exposed to the same pressure and temperature either way. However, if you want your stuff to dry quickly, having stuff below the waterline is probably not going to make an easy day of it.

 

[alphastrength50]

Would baby bottle sterilizers work???

 

[Krampus]

Would baby bottle sterilizers work???

I'm not so familiar with baby bottle sterilizers, but I'm pretty sure it's not so good for our purposes here. Just based on an image search of the web, baby bottle sterilizers appear to be a lower temperature version of a dry heat sterilizer. I expect that sterilization done by these devices is food level. Your digestive process will kill much of the pathogens you may consume, so food doesn't need to have the same level of sterility that something intended to bypass the digestive process would. Not to recommend this method, but a conventional oven would probably do better than the baby bottle sterilizer.