Big dog
New Member
Can anyone suggest an easy way to sterilize 10 ml vials? I was thinking of boiling them.
MESO-Rx
Anabolic Steroids
Sterilizing vials
- Thread starter Big dog
- Start date
Soak in IPA alcohol, then bake in the oven.
Big dog
New Member
Ok thank you...
Big dog
New Member
By IPA you mean isopropyl alcohol?
Oven works best Imo. If you are brewing for yourself, it's better to use pre sterilized vials, and inject your stetile product directly into it.
Good as it gets.
Big dog
New Member
Ok thanks. And before oven do you wash or soak?
T_verde77
New Member
Soaking than baking is better. but I just bake them
Big dog
New Member
Thx what do you soak them in?
bkstacks
New Member
May be over-kill, but it's how I do...
* Rinse thoroughly
* Soak in a bath of anti-bacterial soap and some ISO Alchohol
* Rinse out w/ distilled H2O
* Cover each bottle-top w/ a small piece of aluminum foil and place in the oven @ 350 Degrees via 30-45 min...
* Each should be sterile and ready for some oil.
* Rinse thoroughly
* Soak in a bath of anti-bacterial soap and some ISO Alchohol
* Rinse out w/ distilled H2O
* Cover each bottle-top w/ a small piece of aluminum foil and place in the oven @ 350 Degrees via 30-45 min...
* Each should be sterile and ready for some oil.
Hope the tinfoil is sterilised 
kap73
New Member
I just rinse 4 or 5 times then put foil on vials, bake in oven for 2 hours at 250c
Never had an issue touch wood
Never had an issue touch wood
May be over-kill, but it's how I do...
* Rinse thoroughly
* Soak in a bath of anti-bacterial soap and some ISO Alchohol
* Rinse out w/ distilled H2O
* Cover each bottle-top w/ a small piece of aluminum foil and place in the oven @ 350 Degrees via 30-45 min...
* Each should be sterile and ready for some oil.
If that's 350 degrees F then you're not doing it long enough.
Jakan
New Member
2 hours baking ? Why so long time ?
kap73
New Member
Why not so long? Cant hurt
master.on
New Member
Boiling them in alcohol?
That ain't easy but they will be very sterile.
The easy way would be to buy presterilized vials but they ain't that cheap.
Now if you want a cheap and easy way...
kap73
New Member
Lol
master.on
New Member
Be careful that IPA ain't soaking wet as all alcohols are flammable
Look up depyrogenation tables. You'll see 30-45min is only long enough if you're working with super hot temps like 500+ deg
Jakan
New Member
260celsius for 45 minutes ? Which bacteria or virus is alive after 15minutes in 260deg( 500F ) ?
Think endotoxins and pyrogens. Here is an excerpt straight from the FDA:
"Historically, vials or glass components have been rendered pyrogen-free by dry heat sterilization at high temperatures. Some texts have recommended the depyrogenation of glassware and equipment by heating at a temperature of 250 C for 45 minutes. It has been reported that 650 C for 1 minute or 180 C for 4 hours, likewise, will destroy pyrogens. Studies by Tsuji et al, published in 1978, have shown that at lower temperatures (of 170 C), thermal destruction follows second-order rate, and a 3 log reduction of endotoxin levels at lower temperatures might not be practical."
Bacterial Endotoxins/Pyrogens
