guyfawkes1010
Member
So just re-filtered about 3 bottles of test using whatman .2 syringe filters into new pre-sterilized/sealed vials. Using a 60ml syringe, caulk gun, and being as hygienic as possible while also using a flow hood.
I think I did ok except one problem was when I was done with a vial and pulled out the syringe it kept dripping. Kinda didnt know what to do so recapped, prepped the next vial, uncapped, wiped with iso pad, then filled the next vial. It was kept inside the flow hood the whole time and didnt touch anything so I assume it remained sterile. Also added another 1.5% BA for a total of about 2.7%.
One thing in the future I will do is make sure all the vials are pre-prepped with the vent needle already in and kept under the flow hood so I dont have to stop.
I think I did ok except one problem was when I was done with a vial and pulled out the syringe it kept dripping. Kinda didnt know what to do so recapped, prepped the next vial, uncapped, wiped with iso pad, then filled the next vial. It was kept inside the flow hood the whole time and didnt touch anything so I assume it remained sterile. Also added another 1.5% BA for a total of about 2.7%.
One thing in the future I will do is make sure all the vials are pre-prepped with the vent needle already in and kept under the flow hood so I dont have to stop.