Filtering hgh

[Ghoul]

I don't get being anti filtering. If you don't want to, don't. Why all the hate for it? It's like being mad at people that alcohol swab the injection site.

I used to wonder this as well. Either do it, or if you think it's unnecessary don't. But if you're going to actively argue against it explain the rationale of how it not only doesn't reduce potential risk, but increases it.

The irrational anger that comes from those who do this suggests that since they don't filter, "killing the messenger" is a way to dismiss the risks being brought up. The same reaction you'd expect if you pointed out the hazards of alcohol to a bunch of drunks in a bar.

 

[BigDadd7]

I used to wonder this as well. Either do it, or if you think it's unnecessary don't. But if you're going to actively argue against it explain the rationale of how it not only doesn't reduce potential risk, but increases it.

The irrational anger that comes from those who do this suggests that since they don't filter, "killing the messenger" is a way to dismiss the risks being brought up. The same reaction you'd expect if you pointed out the hazards of alcohol to a bunch of drunks in a bar.

The truth is, this is just a guy thay wants to hate on you. It's not really about being anti-filtering. That would be absurd.

 

[Elektrobot]

Everything you ever done on the forum is spreading lies, missinformation and copy paste chatgpt.

When you don't realize your rhetoric sounds the same regardless of which Username you logged in with

 

[Ghoul]

Everything you ever done on the forum is spreading lies, missinformation and copy paste chatgpt.

I never said i don’t buy from SSA. Im saying your garbage ”filter everything” propaganda bullshit is just sick.

"Sick"? What the fuck are you talking about?

How does filtering "harm" anyone you braindead asshole?

 

[Elektrobot]

"Sick"? What the fuck are you talking about?

How does filtering "harm" anyone you braindead asshole?

I stubbed my vagina on a chair while on the way to get a filter out..... so

 

[sionnach]

"Sick"? What the fuck are you talking about?

How does filtering "harm" anyone you braindead asshole?

A filter farted in his dishwasher.

(Sorry, @Bandern it's everyone's meme now)

 

[Bandern]

A filter farted in his dishwasher.

(Sorry, @Bandern it's everyone's meme now)

Mama I made it!!!

 

[AlexDavis43]

I don't get being anti filtering. If you don't want to, don't. Why all the hate for it? It's like being mad at people that alcohol swab the injection site.

I don't think anyone is anti-filtering, but the conversation started very differently than it is now, and people remember that

It used to be about fear-mongering increased long-term disease risk from glass shards, misfolded proteins, autoimmunity, dementia, heart disease, etc., etc.; ie, lots of bubble boy shit that decades of bodybuilders not filtering stuff debunked

The fear-mongering rhetoric has toned down a bit, probably because at least some of the early promoters aren't completely retarded and realized they were massively over-exaggerating the risks

 

[Freeboomboom]

I don't think anyone is anti-filtering, but the conversation started very differently than it is now, and people remember that

It used to be about fear-mongering increased long-term disease risk from glass shards, misfolded proteins, autoimmunity, dementia, heart disease, etc., etc.; ie, lots of bubble boy shit that decades of bodybuilders not filtering stuff debunked

The fear-mongering rhetoric has toned down a bit, probably because at least some of the early promoters aren't completely retarded and realized they were massively over-exaggerating the risks

He gets it.

 

[Tomahawk54]

I don't think anyone is anti-filtering, but the conversation started very differently than it is now, and people remember that

It used to be about fear-mongering increased long-term disease risk from glass shards, misfolded proteins, autoimmunity, dementia, heart disease, etc., etc.; ie, lots of bubble boy shit that decades of bodybuilders not filtering stuff debunked

The fear-mongering rhetoric has toned down a bit, probably because at least some of the early promoters aren't completely retarded and realized they were massively over-exaggerating the risks

This is where im at with it. I see people talking about immunogenicity and dangers of misfolded proteins in hgh etc.
At this point there is a very large sample size of bodybuilders pinning ugl generic hgh for a long time and very little bad to report from it. Same goes for EO. A lot of fear mongering about EO. How much actual bad has happened from EO? Sure, some people dont react well to it but for the most part it seems like people do fine using some gear with EO on it.
Am i looking for gear with EO in it on purpose? No. However, if im gunna run 200mg/ml primo its probably gunna have EO in it.

 

[Sera]

Some might remember me having issues filtering SSA hgh. After filtration I was having problems with the hgh forming aggregates (look like little strings).

Recently I created a bacteriostatic water with polysorbate 20 in it and buffer salts. This has seemed to solve the problem. I initially thought it might be the pH but after testing it, it seems the pH is perfectly fine already before I even added the buffer (I really should have checked first)

Looking into it surfactants like ps20 can protect hgh from shear stress.

I will be experimenting further to refine the dose of polysorbate 20 and ensure the pH is fine without the added buffer.

 

[sionnach]

Some might remember me having issues filtering SSA hgh. After filtration I was having problems with the hgh forming aggregates (look like little strings).

Recently I created a bacteriostatic water with polysorbate 20 in it and buffer salts. This has seemed to solve the problem. I initially thought it might be the pH but after testing it, it seems the pH is perfectly fine already before I even added the buffer (I really should have checked first)

Looking into it surfactants like ps20 can protect hgh from shear stress.

I will be experimenting further to refine the dose of polysorbate 20 and ensure the pH is fine without the added buffer.

Hmm, that's interesting. Thanks for remembering to keep us updated.

 

[StoopidGoat]

Some might remember me having issues filtering SSA hgh. After filtration I was having problems with the hgh forming aggregates (look like little strings).

Recently I created a bacteriostatic water with polysorbate 20 in it and buffer salts. This has seemed to solve the problem. I initially thought it might be the pH but after testing it, it seems the pH is perfectly fine already before I even added the buffer (I really should have checked first)

Looking into it surfactants like ps20 can protect hgh from shear stress.

I will be experimenting further to refine the dose of polysorbate 20 and ensure the pH is fine without the added buffer.

This is so odd because I also just tested this a few days ago. Are you using refined PS20 or just regular? Are you still filtering after? What v/v % are you adding? Feel free to shoot a DM to share your protocol, got lots of questions.

 

[Sera]

This is so odd because I also just tested this a few days ago. Are you using refined PS20 or just regular? Are you still filtering after? What v/v % are you adding? Feel free to shoot a DM to share your protocol, got lots of questions.

I’m happy to share. I tried 0.2% first off, but will be trying 0.07% next as it seemed a little too much (pretty prone to bubbling, and there’s no need to use more than necessary as seemingly it can contribute to oxidation of the hgh). And honestly I wasn’t even aware there were higher grades until you mentioned it. So I just used standard grade. Hopefully that’s fine for storage for a couple of weeks. Seems higher purities are more critical for liquid formulas that need to be stable for 12-24 months.

Yes of course I’m filtering it, that was the main issue, when I filtered it, aggregates still formed in the resulting liquid, after filtering. That’s why I decided to see if I could solve it as another brand (Lobster) I used did not do that. So I looked into all the factors impacting hgh stability and tried to account for them.

 

[Sera]

Interestingly, I experienced reversible aggregation for the first time.

I pulled my vial of hgh out of the fridge and noticed a cloud of particles and thought “it’s ruined, I must have messed up the water or something”

I put it on the counter after inspecting it (sure enough there was a bunch of particles). I was going to do a post-mortem and analyse the pH to see if that changed in the fridge or something and killed it.

After I had a shower I brought it upstairs and was going to check the pH.
However on inspecting the vial again, I noticed it was crystal clear!
I then remembered the insert from Serostim, where it mentioned something about particles after it had been in the fridge that would reverse.
Very interesting!

Possibly because the pH was slightly too low this time.

 

[Ateam2023]

Interestingly, I experienced reversible aggregation for the first time.

I pulled my vial of hgh out of the fridge and noticed a cloud of particles and thought “it’s ruined, I must have messed up the water or something”

I put it on the counter after inspecting it (sure enough there was a bunch of particles). I was going to do a post-mortem and analyse the pH to see if that changed in the fridge or something and killed it.

After I had a shower I brought it upstairs and was going to check the pH.
However on inspecting the vial again, I noticed it was crystal clear!
I then remembered the insert from Serostim, where it mentioned something about particles after it had been in the fridge that would reverse.
Very interesting!

Possibly because the pH was slightly too low this time.

did you happen to measure the ph this time?

 

[vulpes]

I am curious to know -does anyone know what filtering does to the molecule? People say be careful with HGH, don't shake it up. The pressure needed to put it through a filter might not do a delicate molecule any good. Have people tested this to make sure? If so, the molecule must be stronger than some people say, which is itself interesting.

 

[Sera]

did you happen to measure the ph this time?

I have measured this vial before, it was 6.5. Since it cleared up and it was late I just decided to not bother testing it again. I might do some other time.

 

[Ghoul]

I am curious to know -does anyone know what filtering does to the molecule? People say be careful with HGH, don't shake it up. The pressure needed to put it through a filter might not do a delicate molecule any good. Have people tested this to make sure? If so, the molecule must be stronger than some people say, which is itself interesting.

Yes, this has been extensively tested by one of the pioneers of protein/pepitde drugs, and a world leading scientist in the area of pharmaceutical formulations and delivery devices.

Filtering by hand through .22um PES filters does not damage rHGH.

In manufacturing lines, it was discovered rHGH pumped at high pressure through narrow metal tubes / needles. caused "sheer stress" (getting physically caught in metal imperfections in tube walls temporarily pulling apart and unfolding the protein) followed by exposure to air (via turbulence / foaming / bubbles) increased the final product's tendency to aggregate. Apparently the unfolding made parts of rHGH normally protected against oxidation in the folds vulnerable to brief contact with air, instantly oxidizing those parts. This damaged rHGH is less stable and sticks to other rHGH molecules forming aggregates,

It's difficult to translate those industrial pressures into something relevant to hand use of syringes, but to be on the safe side, when handling peptides I think it's better to use a large gauge needles (18-25) to keep pressure low and minimize any risk of sheer stress.

 

[Sera]

I am curious to know -does anyone know what filtering does to the molecule? People say be careful with HGH, don't shake it up. The pressure needed to put it through a filter might not do a delicate molecule any good. Have people tested this to make sure? If so, the molecule must be stronger than some people say, which is itself interesting.

You need surfactants to protect it during filtration. Otherwise it adsorbs slightly to the membrane and unfolds.